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The map label for this gene is thiD [H]

Identifier: 38232677

GI number: 38232677

Start: 33081

End: 33899

Strand: Direct

Name: thiD [H]

Synonym: DIP0035

Alternate gene names: 38232677

Gene position: 33081-33899 (Clockwise)

Preceding gene: 38232676

Following gene: 38232678

Centisome position: 1.33

GC content: 60.2

Gene sequence:

>819_bases
ATGACCCCACACATACTCACCATCGCAGGATCCGACCCCTCCGGCGGGGCCGGAATCCAAGCAGACCTCAAATCCATCAT
GGCAGCCGGCGGCTACGGCATGGCAGCAATCACCGCCCTGACCGCGCAAAACACCTGCGGAGTTACCGCAATACACACCC
CACCCACGGAGTTCCTCAGCGAACAACTCCGCGCAATATCCGACGACATCACCATCCATGCCATCAAAATCGGCATGATC
GGATCCTCTGATGCAGCCACCGCCATCGCCACATGGCTTGACCAACTACACCACACATCCATCGTGGTTCTCGACCCAGT
CATGGTAGCCACCTCCGGATCGGTACTTGGCCAACGTCATTATTTTGAACCCCTGCTCCACCACGCCACCGTGATCACCC
CCAACCTGCCAGAACTCGCAGTCCTGGCAAACAACCACGGCCCTGAACAGGCCGAACATGCCGCACGCAGCCTCGCAGAA
CAATACGACTGTGCAGTGCTGCTCAAAGGTGGGCACCGACACGGAACCGACGACCTTGGCAACACCTGGATTACTGCCAG
TGGCCCCCAATTCCACGCACCCAGCCCCCGCATCCACACCACCGACACCCACGGCACCGGCTGCTCGCTCTCCTCAGCAC
TCGCCACCCGGCTTGCCATCGAACCCCCAGAACCCGCCCTCCACTGGGCTACCACTTGGCTCAACGGGGCAATCGCCCAC
GGCAGTGACCTCAACGTAGGCCACGGCAACGGTCCTGTCGACCACAGCTACCAGCTAAGGGAATACTCAGCTAACTGCAA
CACAATCAATGCACCCTAA

Upstream 100 bases:

>100_bases
GTGCCTCTGGCATAAGAAGTGCCGCATTTATCGAACAATTCGCACACCTTAACCCACACCTCACCTTCCACAACGTCCCC
AGCGGAATCAACGAACTACC

Downstream 100 bases:

>100_bases
AACCGGAAGTGCCACCACACGGGGGTATTTCGAAGGATTTTTCTCCGAACTTCACACCAAATCGCTTCCAAACCGGCCGT
TTGACTGGATTTGGTGTGAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTPHILTIAGSDPSGGAGIQADLKSIMAAGGYGMAAITALTAQNTCGVTAIHTPPTEFLSEQLRAISDDITIHAIKIGMI
GSSDAATAIATWLDQLHHTSIVVLDPVMVATSGSVLGQRHYFEPLLHHATVITPNLPELAVLANNHGPEQAEHAARSLAE
QYDCAVLLKGGHRHGTDDLGNTWITASGPQFHAPSPRIHTTDTHGTGCSLSSALATRLAIEPPEPALHWATTWLNGAIAH
GSDLNVGHGNGPVDHSYQLREYSANCNTINAP

Sequences:

>Translated_272_residues
MTPHILTIAGSDPSGGAGIQADLKSIMAAGGYGMAAITALTAQNTCGVTAIHTPPTEFLSEQLRAISDDITIHAIKIGMI
GSSDAATAIATWLDQLHHTSIVVLDPVMVATSGSVLGQRHYFEPLLHHATVITPNLPELAVLANNHGPEQAEHAARSLAE
QYDCAVLLKGGHRHGTDDLGNTWITASGPQFHAPSPRIHTTDTHGTGCSLSSALATRLAIEPPEPALHWATTWLNGAIAH
GSDLNVGHGNGPVDHSYQLREYSANCNTINAP
>Mature_271_residues
TPHILTIAGSDPSGGAGIQADLKSIMAAGGYGMAAITALTAQNTCGVTAIHTPPTEFLSEQLRAISDDITIHAIKIGMIG
SSDAATAIATWLDQLHHTSIVVLDPVMVATSGSVLGQRHYFEPLLHHATVITPNLPELAVLANNHGPEQAEHAARSLAEQ
YDCAVLLKGGHRHGTDDLGNTWITASGPQFHAPSPRIHTTDTHGTGCSLSSALATRLAIEPPEPALHWATTWLNGAIAHG
SDLNVGHGNGPVDHSYQLREYSANCNTINAP

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=43.3460076045627, Blast_Score=170, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325378, Length=279, Percent_Identity=32.9749103942652, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=276, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=34.1818181818182, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28470; Mature: 28339

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPHILTIAGSDPSGGAGIQADLKSIMAAGGYGMAAITALTAQNTCGVTAIHTPPTEFLS
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEECCHHHHHH
EQLRAISDDITIHAIKIGMIGSSDAATAIATWLDQLHHTSIVVLDPVMVATSGSVLGQRH
HHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCEEEECCCCHHHHHH
YFEPLLHHATVITPNLPELAVLANNHGPEQAEHAARSLAEQYDCAVLLKGGHRHGTDDLG
HHHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NTWITASGPQFHAPSPRIHTTDTHGTGCSLSSALATRLAIEPPEPALHWATTWLNGAIAH
CEEEEECCCCCCCCCCCEECCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHCCEEEC
GSDLNVGHGNGPVDHSYQLREYSANCNTINAP
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TPHILTIAGSDPSGGAGIQADLKSIMAAGGYGMAAITALTAQNTCGVTAIHTPPTEFLS
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEEEECCHHHHHH
EQLRAISDDITIHAIKIGMIGSSDAATAIATWLDQLHHTSIVVLDPVMVATSGSVLGQRH
HHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCEEEECCCCHHHHHH
YFEPLLHHATVITPNLPELAVLANNHGPEQAEHAARSLAEQYDCAVLLKGGHRHGTDDLG
HHHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
NTWITASGPQFHAPSPRIHTTDTHGTGCSLSSALATRLAIEPPEPALHWATTWLNGAIAH
CEEEEECCCCCCCCCCCEECCCCCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHCCEEEC
GSDLNVGHGNGPVDHSYQLREYSANCNTINAP
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]