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The map label for this gene is thiG

Identifier: 38232675

GI number: 38232675

Start: 31289

End: 32074

Strand: Direct

Name: thiG

Synonym: DIP0033

Alternate gene names: 38232675

Gene position: 31289-32074 (Clockwise)

Preceding gene: 38232674

Following gene: 38232676

Centisome position: 1.26

GC content: 63.87

Gene sequence:

>786_bases
ATGCTCACCATCGCAGACCGCAGCTTCCAATCCCACCTCATCATGGGAACCGGTGGCGCAAGCTCCTTTGACACCCTAGA
AAAATCCCTCATCGCCTCCGGAACCGAACTCACCACCGTAGCGATGCGTCGACACGCCGCCCACACCGGAGCCCACGGCG
AATCCGTCTTCGAACTCATGCAGCGCCTCAACATCACCCCACTACCCAACACCGCAGGCTGCCGCACCGCCCGCGACGCC
ATCCTCACCGCACAACTCGCCCGCGAAGCACTCGACACCTCATGGATCAAAGTGGAAGTCATCGCCGACGACACCACCTT
GCTCCCCGACGTCCTCGAACTTATCGACGCCACCGAAACCCTCACCAACGACGGATTCACCGTCCTCGCCTACACCTCCG
ACGACCCCGTAGTCGCACAACGACTCGAAGACGCCGGCGCCGCCGCAGTCATGCCCCTAGGATCACCCATCGGAACCGGA
CTCGGCATCCTCAACCCCCACAACATCGAACTCATCTGCTCACGCGCCACAGTCCCCGTACTCCTCGACGCCGGCATCGG
AACCGCCTCCGACGCCACCCTCGCCATGGAACTCGGCTGCTCAGGCGTACTCCTCGCCAGCGCCATCAACCGATGCATCA
ACCCCATCACCATGGCCACCGCCATGAAACACGCCGTCGAAGCAGGACGACTCGCGCGCGAAGCCGGGCGCATACCGCGC
CGGGAGCATGCGGTGGCGTCGTCAAGCTTTGAAGGGCTCGCCTCCTGGGCGGACGAGGTGCTGTGA

Upstream 100 bases:

>100_bases
GCCATCAACCAACGCGTGATACCCCGCTCACAATGGGACACCACCACAGTCACCGCAGGCGACCACCTCGACATTCTCAC
CGCAGTGCAGGGAGGATAAC

Downstream 100 bases:

>100_bases
TGCTCGACGAGCTGGAACGCCAACGCGTAGCCCGCCAACTGCGGCTACCTGGTTTTGGGATTGAGCAGCAAGAACGGCTC
AACAACGGGCGAGTGCTGGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLTIADRSFQSHLIMGTGGASSFDTLEKSLIASGTELTTVAMRRHAAHTGAHGESVFELMQRLNITPLPNTAGCRTARDA
ILTAQLAREALDTSWIKVEVIADDTTLLPDVLELIDATETLTNDGFTVLAYTSDDPVVAQRLEDAGAAAVMPLGSPIGTG
LGILNPHNIELICSRATVPVLLDAGIGTASDATLAMELGCSGVLLASAINRCINPITMATAMKHAVEAGRLAREAGRIPR
REHAVASSSFEGLASWADEVL

Sequences:

>Translated_261_residues
MLTIADRSFQSHLIMGTGGASSFDTLEKSLIASGTELTTVAMRRHAAHTGAHGESVFELMQRLNITPLPNTAGCRTARDA
ILTAQLAREALDTSWIKVEVIADDTTLLPDVLELIDATETLTNDGFTVLAYTSDDPVVAQRLEDAGAAAVMPLGSPIGTG
LGILNPHNIELICSRATVPVLLDAGIGTASDATLAMELGCSGVLLASAINRCINPITMATAMKHAVEAGRLAREAGRIPR
REHAVASSSFEGLASWADEVL
>Mature_261_residues
MLTIADRSFQSHLIMGTGGASSFDTLEKSLIASGTELTTVAMRRHAAHTGAHGESVFELMQRLNITPLPNTAGCRTARDA
ILTAQLAREALDTSWIKVEVIADDTTLLPDVLELIDATETLTNDGFTVLAYTSDDPVVAQRLEDAGAAAVMPLGSPIGTG
LGILNPHNIELICSRATVPVLLDAGIGTASDATLAMELGCSGVLLASAINRCINPITMATAMKHAVEAGRLAREAGRIPR
REHAVASSSFEGLASWADEVL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=45.8498023715415, Blast_Score=214, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_CORDI (Q6NKI6)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938442.1
- ProteinModelPortal:   Q6NKI6
- SMR:   Q6NKI6
- GeneID:   2650185
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0033
- NMPDR:   fig|257309.1.peg.33
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q6NKI6
- ProtClustDB:   PRK00208
- BioCyc:   CDIP257309:DIP0033-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27370; Mature: 27370

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 97-97 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIADRSFQSHLIMGTGGASSFDTLEKSLIASGTELTTVAMRRHAAHTGAHGESVFELM
CEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QRLNITPLPNTAGCRTARDAILTAQLAREALDTSWIKVEVIADDTTLLPDVLELIDATET
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LTNDGFTVLAYTSDDPVVAQRLEDAGAAAVMPLGSPIGTGLGILNPHNIELICSRATVPV
HCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEE
LLDAGIGTASDATLAMELGCSGVLLASAINRCINPITMATAMKHAVEAGRLAREAGRIPR
EEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
REHAVASSSFEGLASWADEVL
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTIADRSFQSHLIMGTGGASSFDTLEKSLIASGTELTTVAMRRHAAHTGAHGESVFELM
CEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QRLNITPLPNTAGCRTARDAILTAQLAREALDTSWIKVEVIADDTTLLPDVLELIDATET
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LTNDGFTVLAYTSDDPVVAQRLEDAGAAAVMPLGSPIGTGLGILNPHNIELICSRATVPV
HCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEE
LLDAGIGTASDATLAMELGCSGVLLASAINRCINPITMATAMKHAVEAGRLAREAGRIPR
EEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
REHAVASSSFEGLASWADEVL
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910