Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 38232647

GI number: 38232647

Start: 5379

End: 7436

Strand: Direct

Name: gyrB [H]

Synonym: DIP0005

Alternate gene names: 38232647

Gene position: 5379-7436 (Clockwise)

Preceding gene: 38232646

Following gene: 38232651

Centisome position: 0.22

GC content: 56.85

Gene sequence:

>2058_bases
GTGCACGCTTCCGTGGCAACCGCTGAACATGAATATGGCGCCTCATCCATTACGATCCTCGAGGGTCTGGAGGCCGTCCG
CAAGCGTCCCGGTATGTACATCGGTTCTACCGGTGAACGCGGCCTGCACCACCTAGTGTGGGAGGTCGTGGATAACTCCG
TCGACGAGGCAATGGCCGGTTATGCTACCCACGTCGATGTGACCCTGCTTGCCGACGGCGGCGTGGAGGTCGTGGATAAC
GGCCGTGGTATCCCCGTTGAAATGCACCCATCTGGTGCGCCGACCGTCCAAGTCGTTATGACCCAGCTGCACGCAGGTGG
CAAGTTCGACTCCGATTCCTACGCGGTTTCCGGTGGTCTACACGGTGTGGGTATCTCCGTCGTGAACGCCCTGTCTACCC
GTGTGGAGGCAGACATCAAACGTGATGGCAAGCACTGGCTGCAGAACTTCTCCATGGCCATCCCTGATCCGCTGGTCGAG
GGCGGTAATGCTCGTGGCACCGGTACCACCATCCGCTTCTGGCCAGATGCGGAGATCTTTGAAACGACCACCTTCAAGTT
TGAGACGATCAGCCGACGTCTCCAAGAAATGGCCTTCTTGAACAAGGGTCTGACCATTACGCTGGTAGACAAGCGCGTTA
CTGACGAGGAACTTGAACTCGAAGCGATCGCCGAAGAAGGCGACACCGCTGAAAACGTTTCTTTGGACCAAATCGATGTC
GATGCCGACGGCAACATCGATGCACCTGCTGCACCAAAGAAACGCGAGAAGAAGAAGGTTTTCTTCTACCCAGATGGTTT
GAAGGACTACGTTGCACACCTGAACAAGTCCAAGCAGGTTATCCACCCCACCATCATCTCCTTCGACGCCAAGGGCAACG
ACCACGAGGTTGAGGTGGCAATGCAGTGGAACAACAGCTACTCGCAGAGCGTGCACACCTTCGCTAACACCATTAACACG
TTTGAAGGTGGCACCCACGAAGAGGGCTTCCGTGCCGCGCTGACGTCGTTGATGAACCGCTACGCTCGTGAGCACAAGCT
GCTCAAGGAAAAGGAAGCAAACCTTACGGGTGACGACTGCCGTGAGGGCCTTTCCGCCGTGATCTCCGTGCGCGTTGGTG
ATCCGCAGTTCGAGGGCCAGACCAAGACCAAACTTGGAAACACTGAGGTCAAGGGCTTTGTTCAGCGTATGGTCAATGAG
CACATTGCTGATTGGCTGGATGCTAACCCTGCAGAAGCCAAGACGATCATCAACAAAGCTGTGTCCTCGGCCCATGCGCG
CGTGGCGGCTCGTAAGGCGCGCGATTTGGTGCGTCGTAAGTCGGCCACCGACCTCGGCGGCCTGCCCGGTAAGCTCGCTG
ATTGCCGCTCCAAGGACCCAGAAAAGTCCGAACTGTATATCGTGGAGGGTGACTCCGCAGGTGGTTCCGCTAAGGCTGGC
CGTGATTCCCTCTACCAGGCAATTCTTCCGCTGCGCGGTAAGATCCTGAACGTGGAGAAGGCACGTTTGGACAAGGTGCT
TAAGAACGCCGAGGTCCAAGCGATCATCACCGCTTTGGGTACCGGCATTCATGATGAGTTTGACATTAAGAAGCTGCGCT
ACCACAAGATCGTGCTGATGGCCGACGCCGATGTCGACGGCTCTCACATCGCAACCTTGCTGCTCACCTTGCTGTTCCGT
TTCATGCCACAGCTGATCGAAGAAGGCCACGTCTACCTCGCGCAGCCACCGCTCTACAAACTTAAGTGGGGCAAGGGTGA
GCCAGGCTTTGCTTACTCTGATGCGGAACGCGACACCTTGCTTGCTGAGGGCTTGGCGCAAAACCGCAAGATCAACAAGG
ACGACGGCATCCAGCGTTACAAAGGTCTAGGCGAGATGAACGCCTCTGAGCTGTGGGAAACCACACTTGATCCGAAGTAC
CGCGTCCTGCGTCGCGTGGACATTACCGACGCACAGCGTGCCGACGAGATCTTCTCCATCCTCATGGGTGACGACGTATC
TGCACGCCGTAGCTTTATTACCCGCCGTGCTAAGGACGTCCGTTTCTTGGACATCTAG

Upstream 100 bases:

>100_bases
GGCGCTGTGTAGCCTGTGGGGCGTGTGCGAGGGGCTACCTAGTGGGGAGGTAGGCAGTGTAATATGGGAGAGTCTAGGCT
CAAATCTCGCATAGCAAGGA

Downstream 100 bases:

>100_bases
CATGACGCTAGCATGACGAAGGCCCGCCGCAGAGTGTTTCTGTAGCGGGCCTTCGTAGTGGGGGAGTTACTTCATGTAGT
TCTGCATGATTTGTCCCAAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MHASVATAEHEYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYATHVDVTLLADGGVEVVDN
GRGIPVEMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKRDGKHWLQNFSMAIPDPLVE
GGNARGTGTTIRFWPDAEIFETTTFKFETISRRLQEMAFLNKGLTITLVDKRVTDEELELEAIAEEGDTAENVSLDQIDV
DADGNIDAPAAPKKREKKKVFFYPDGLKDYVAHLNKSKQVIHPTIISFDAKGNDHEVEVAMQWNNSYSQSVHTFANTINT
FEGGTHEEGFRAALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEVKGFVQRMVNE
HIADWLDANPAEAKTIINKAVSSAHARVAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKAG
RDSLYQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGSHIATLLLTLLFR
FMPQLIEEGHVYLAQPPLYKLKWGKGEPGFAYSDAERDTLLAEGLAQNRKINKDDGIQRYKGLGEMNASELWETTLDPKY
RVLRRVDITDAQRADEIFSILMGDDVSARRSFITRRAKDVRFLDI

Sequences:

>Translated_685_residues
MHASVATAEHEYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYATHVDVTLLADGGVEVVDN
GRGIPVEMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKRDGKHWLQNFSMAIPDPLVE
GGNARGTGTTIRFWPDAEIFETTTFKFETISRRLQEMAFLNKGLTITLVDKRVTDEELELEAIAEEGDTAENVSLDQIDV
DADGNIDAPAAPKKREKKKVFFYPDGLKDYVAHLNKSKQVIHPTIISFDAKGNDHEVEVAMQWNNSYSQSVHTFANTINT
FEGGTHEEGFRAALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEVKGFVQRMVNE
HIADWLDANPAEAKTIINKAVSSAHARVAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKAG
RDSLYQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGSHIATLLLTLLFR
FMPQLIEEGHVYLAQPPLYKLKWGKGEPGFAYSDAERDTLLAEGLAQNRKINKDDGIQRYKGLGEMNASELWETTLDPKY
RVLRRVDITDAQRADEIFSILMGDDVSARRSFITRRAKDVRFLDI
>Mature_685_residues
MHASVATAEHEYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYATHVDVTLLADGGVEVVDN
GRGIPVEMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRVEADIKRDGKHWLQNFSMAIPDPLVE
GGNARGTGTTIRFWPDAEIFETTTFKFETISRRLQEMAFLNKGLTITLVDKRVTDEELELEAIAEEGDTAENVSLDQIDV
DADGNIDAPAAPKKREKKKVFFYPDGLKDYVAHLNKSKQVIHPTIISFDAKGNDHEVEVAMQWNNSYSQSVHTFANTINT
FEGGTHEEGFRAALTSLMNRYAREHKLLKEKEANLTGDDCREGLSAVISVRVGDPQFEGQTKTKLGNTEVKGFVQRMVNE
HIADWLDANPAEAKTIINKAVSSAHARVAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKAG
RDSLYQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGSHIATLLLTLLFR
FMPQLIEEGHVYLAQPPLYKLKWGKGEPGFAYSDAERDTLLAEGLAQNRKINKDDGIQRYKGLGEMNASELWETTLDPKY
RVLRRVDITDAQRADEIFSILMGDDVSARRSFITRRAKDVRFLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=674, Percent_Identity=24.9258160237389, Blast_Score=122, Evalue=2e-27,
Organism=Homo sapiens, GI19913408, Length=357, Percent_Identity=28.8515406162465, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI48994957, Length=583, Percent_Identity=51.114922813036, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789408, Length=675, Percent_Identity=39.4074074074074, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17535065, Length=629, Percent_Identity=24.483306836248, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=590, Percent_Identity=26.1016949152542, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=208, Percent_Identity=32.6923076923077, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=705, Percent_Identity=25.3900709219858, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17136538, Length=600, Percent_Identity=25.3333333333333, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 75485; Mature: 75485

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHASVATAEHEYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAG
CCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YATHVDVTLLADGGVEVVDNGRGIPVEMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGL
CEEEEEEEEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSTRVEADIKRDGKHWLQNFSMAIPDPLVEGGNARGTGTTIRFWPDAEIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCCHH
ETTTFKFETISRRLQEMAFLNKGLTITLVDKRVTDEELELEAIAEEGDTAENVSLDQIDV
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEE
DADGNIDAPAAPKKREKKKVFFYPDGLKDYVAHLNKSKQVIHPTIISFDAKGNDHEVEVA
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEECCEEEEEECCCCCCEEEEE
MQWNNSYSQSVHTFANTINTFEGGTHEEGFRAALTSLMNRYAREHKLLKEKEANLTGDDC
EEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
REGLSAVISVRVGDPQFEGQTKTKLGNTEVKGFVQRMVNEHIADWLDANPAEAKTIINKA
HHHHHHEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSSAHARVAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
RDSLYQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDIKKLRYHKIVLM
HHHHHHHHHHHCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHEEEEEEEEE
ADADVDGSHIATLLLTLLFRFMPQLIEEGHVYLAQPPLYKLKWGKGEPGFAYSDAERDTL
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHH
LAEGLAQNRKINKDDGIQRYKGLGEMNASELWETTLDPKYRVLRRVDITDAQRADEIFSI
HHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
LMGDDVSARRSFITRRAKDVRFLDI
HHCCCHHHHHHHHHHHHCCCEEECC
>Mature Secondary Structure
MHASVATAEHEYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAG
CCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YATHVDVTLLADGGVEVVDNGRGIPVEMHPSGAPTVQVVMTQLHAGGKFDSDSYAVSGGL
CEEEEEEEEEECCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSTRVEADIKRDGKHWLQNFSMAIPDPLVEGGNARGTGTTIRFWPDAEIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCCHH
ETTTFKFETISRRLQEMAFLNKGLTITLVDKRVTDEELELEAIAEEGDTAENVSLDQIDV
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEE
DADGNIDAPAAPKKREKKKVFFYPDGLKDYVAHLNKSKQVIHPTIISFDAKGNDHEVEVA
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEECCEEEEEECCCCCCEEEEE
MQWNNSYSQSVHTFANTINTFEGGTHEEGFRAALTSLMNRYAREHKLLKEKEANLTGDDC
EEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
REGLSAVISVRVGDPQFEGQTKTKLGNTEVKGFVQRMVNEHIADWLDANPAEAKTIINKA
HHHHHHEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSSAHARVAARKARDLVRRKSATDLGGLPGKLADCRSKDPEKSELYIVEGDSAGGSAKAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
RDSLYQAILPLRGKILNVEKARLDKVLKNAEVQAIITALGTGIHDEFDIKKLRYHKIVLM
HHHHHHHHHHHCCCEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHEEEEEEEEE
ADADVDGSHIATLLLTLLFRFMPQLIEEGHVYLAQPPLYKLKWGKGEPGFAYSDAERDTL
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHH
LAEGLAQNRKINKDDGIQRYKGLGEMNASELWETTLDPKYRVLRRVDITDAQRADEIFSI
HHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHH
LMGDDVSARRSFITRRAKDVRFLDI
HHCCCHHHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8878580 [H]