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The map label for this gene is recF

Identifier: 38232645

GI number: 38232645

Start: 3542

End: 4735

Strand: Direct

Name: recF

Synonym: DIP0003

Alternate gene names: 38232645

Gene position: 3542-4735 (Clockwise)

Preceding gene: 161598680

Following gene: 38232646

Centisome position: 0.14

GC content: 54.27

Gene sequence:

>1194_bases
GTGTACATTCGTGAGCTTTCGCTTCGAGATTTCCGCTCGTGGCCAGAGTGCACTGTCACTTTGGAACCTGGGGTGACGCT
TTTTGTTGGCCGTAATGGTTTTGGCAAAACGAACATTGTTGAGGCCATCGGCTATGTGGCTCATCTGGGTTCTCACCGTG
TGTTTCACGATTCTGCGCTGGTGCGTCAAGGAAAAGAATCGGCTCGTGTGTCGGTAACAGCGGTTAACCACGGCCGCGAG
CTCACCGCGCATCTGTTGATCAAAGCTAAGGGAGCTAACCAAGCTCAGATCAATCGAACTCGGCTAAAAAGCCCTCGTGA
GCTGCTTGGTGTTGTTAAAACGGTTCTGTTTTCTCCTGAAGATTTGAGCCTTGTTCGTGGCGATCCGGCAGAGCGTCGAC
GCTATCTTGACCATGTGATTGCTACACGCAAACCCCGCCTTGGCGGTGTGAAAGCAGACTATGACAAGGTGCTGCGGCAG
CGTAACTCGTTGCTCAAAACAGCAGGTGCTGCTTTGCGTCGTGGCTATGGCGCTGATGATGGTGCGCTTTCTACCTTGGA
TGTGTGGGATTCCCAGTTGGCACGTCTTGGTGGGCAGCTCATTCATGCACGTCATAGTGTGGTTCGGGAGTTAGGTCCGC
TGGTGCATGATGCCTATGCGCGTATTGCTCCGGAGTCGCGTCCTGCTCATATTCGCTATGTGTCGACGGTGCCGTTTGCG
GATGTTGTTGAGTTGCCGAGCCCCGAAGAATTCGAGGCCGCTATGCTTGCTGAGCTGGGGCAATGTCGGGATAAAGAAAT
TGATCGTGGAGTGTCGCTGGTGGGGCCACACCGTGATGATCTTGATGTTGTCTTGGGTGATTACCCTGCGAAGGGGTTTG
CGAGCCATGGGGAAACGTGGTCGATGTGTTTGTCGTTGCGCCTAGCTGAGTTTCATTTGCTGCGTAATGATGGCACCGAT
CCTGTGCTTATTCTCGATGATGTGTTTGCGGAGTTGGATACGCAGCGTCGTGAGAAGTTGGTGAGTGTGACTGCCGAGGC
TGAGCAGGTGCTCATCACTGCCGCGGTGGGTGATGATCTGCCCGATACTCTTACGGAGTCGGCGGTGCATCGTCACTTTG
TCAGTGTTGCGGACACTCCTGAGGGACGTATTTCGCTTCTCGACGCCGCCACGGGGGTACCACATGACGGATAA

Upstream 100 bases:

>100_bases
CGTACCCTACGCCAGATACCGAATTCACCTACCTGCTCATGCCAGTTCGACTGCCAGGATAAAAACACTCTCTGACTAGT
GCAAAGGACGTCGCCCCACC

Downstream 100 bases:

>100_bases
TGATGCGGTTTCTGCTGCTTTTGCACATATGCGCCAGGAGGCGAAAAAACGCACAGGCACTGTTCCGAACCTGAACAGGC
CGTTGCCGAAGGCGTCGAAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYIRELSLRDFRSWPECTVTLEPGVTLFVGRNGFGKTNIVEAIGYVAHLGSHRVFHDSALVRQGKESARVSVTAVNHGRE
LTAHLLIKAKGANQAQINRTRLKSPRELLGVVKTVLFSPEDLSLVRGDPAERRRYLDHVIATRKPRLGGVKADYDKVLRQ
RNSLLKTAGAALRRGYGADDGALSTLDVWDSQLARLGGQLIHARHSVVRELGPLVHDAYARIAPESRPAHIRYVSTVPFA
DVVELPSPEEFEAAMLAELGQCRDKEIDRGVSLVGPHRDDLDVVLGDYPAKGFASHGETWSMCLSLRLAEFHLLRNDGTD
PVLILDDVFAELDTQRREKLVSVTAEAEQVLITAAVGDDLPDTLTESAVHRHFVSVADTPEGRISLLDAATGVPHDG

Sequences:

>Translated_397_residues
MYIRELSLRDFRSWPECTVTLEPGVTLFVGRNGFGKTNIVEAIGYVAHLGSHRVFHDSALVRQGKESARVSVTAVNHGRE
LTAHLLIKAKGANQAQINRTRLKSPRELLGVVKTVLFSPEDLSLVRGDPAERRRYLDHVIATRKPRLGGVKADYDKVLRQ
RNSLLKTAGAALRRGYGADDGALSTLDVWDSQLARLGGQLIHARHSVVRELGPLVHDAYARIAPESRPAHIRYVSTVPFA
DVVELPSPEEFEAAMLAELGQCRDKEIDRGVSLVGPHRDDLDVVLGDYPAKGFASHGETWSMCLSLRLAEFHLLRNDGTD
PVLILDDVFAELDTQRREKLVSVTAEAEQVLITAAVGDDLPDTLTESAVHRHFVSVADTPEGRISLLDAATGVPHDG
>Mature_397_residues
MYIRELSLRDFRSWPECTVTLEPGVTLFVGRNGFGKTNIVEAIGYVAHLGSHRVFHDSALVRQGKESARVSVTAVNHGRE
LTAHLLIKAKGANQAQINRTRLKSPRELLGVVKTVLFSPEDLSLVRGDPAERRRYLDHVIATRKPRLGGVKADYDKVLRQ
RNSLLKTAGAALRRGYGADDGALSTLDVWDSQLARLGGQLIHARHSVVRELGPLVHDAYARIAPESRPAHIRYVSTVPFA
DVVELPSPEEFEAAMLAELGQCRDKEIDRGVSLVGPHRDDLDVVLGDYPAKGFASHGETWSMCLSLRLAEFHLLRNDGTD
PVLILDDVFAELDTQRREKLVSVTAEAEQVLITAAVGDDLPDTLTESAVHRHFVSVADTPEGRISLLDAATGVPHDG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=26.3440860215054, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CORDI (Q6NKL5)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938412.1
- ProteinModelPortal:   Q6NKL5
- GeneID:   2650258
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0003
- NMPDR:   fig|257309.1.peg.3
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q6NKL5
- ProtClustDB:   PRK00064
- BioCyc:   CDIP257309:DIP0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43591; Mature: 43591

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIRELSLRDFRSWPECTVTLEPGVTLFVGRNGFGKTNIVEAIGYVAHLGSHRVFHDSAL
CCCCCCCHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHH
VRQGKESARVSVTAVNHGRELTAHLLIKAKGANQAQINRTRLKSPRELLGVVKTVLFSPE
HHCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCC
DLSLVRGDPAERRRYLDHVIATRKPRLGGVKADYDKVLRQRNSLLKTAGAALRRGYGADD
CCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GALSTLDVWDSQLARLGGQLIHARHSVVRELGPLVHDAYARIAPESRPAHIRYVSTVPFA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCH
DVVELPSPEEFEAAMLAELGQCRDKEIDRGVSLVGPHRDDLDVVLGDYPAKGFASHGETW
HHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCEEEECCCCCCCCCCCCCHH
SMCLSLRLAEFHLLRNDGTDPVLILDDVFAELDTQRREKLVSVTAEAEQVLITAAVGDDL
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
PDTLTESAVHRHFVSVADTPEGRISLLDAATGVPHDG
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MYIRELSLRDFRSWPECTVTLEPGVTLFVGRNGFGKTNIVEAIGYVAHLGSHRVFHDSAL
CCCCCCCHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHH
VRQGKESARVSVTAVNHGRELTAHLLIKAKGANQAQINRTRLKSPRELLGVVKTVLFSPE
HHCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCC
DLSLVRGDPAERRRYLDHVIATRKPRLGGVKADYDKVLRQRNSLLKTAGAALRRGYGADD
CCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GALSTLDVWDSQLARLGGQLIHARHSVVRELGPLVHDAYARIAPESRPAHIRYVSTVPFA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCH
DVVELPSPEEFEAAMLAELGQCRDKEIDRGVSLVGPHRDDLDVVLGDYPAKGFASHGETW
HHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCEEEECCCCCCCCCCCCCHH
SMCLSLRLAEFHLLRNDGTDPVLILDDVFAELDTQRREKLVSVTAEAEQVLITAAVGDDL
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
PDTLTESAVHRHFVSVADTPEGRISLLDAATGVPHDG
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910