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The map label for this gene is dnaN [H]

Identifier: 161598680

GI number: 161598680

Start: 2316

End: 3503

Strand: Direct

Name: dnaN [H]

Synonym: DIP0002

Alternate gene names: 161598680

Gene position: 2316-3503 (Clockwise)

Preceding gene: 38232643

Following gene: 38232645

Centisome position: 0.09

GC content: 57.15

Gene sequence:

>1188_bases
ATGGACGCACACGCTGTCTCATTCCGGGTCGAAAAAGACGACCTTTCCGGTGCGGTGAGCTGGGTTGCACGTAATTTGCC
CACCAAACCCACGCAGCCGGTCTTGCGCGCGGTTGTCATCACCGCCGATGACGAGGGACTCGAGTTCGCTGGTTTTGATT
ACGAAGTTTCTACAAAAGTCCATATCAACGCGGATGTTCGCCAGCCAGGTCGTATCGCCGTAGCCGGCAAGCTGATGTCG
GACATCATCGGCACCCTGCCGAACAAGACCATTGAGGTTTTCGTCGAAGGCACTCAGGTGCAGGTCGTATGCGGTTCTTC
CCGCTTCGAGCTTCCCCTGATTCCTCTCGACGAGTACCCACCGTTACCACAGTTGCCAGCGGTAACCGGTGCCATCGACC
CGAATTTGTTCACGGATGCGGTGCTGCAAGTTGCTGCGGCCGCTGGTCGCGATGAGACGCTTCCAATGCTCACGGGTATC
CACATGGAGATCGACGGCGAGAACGTCACCTTGACCGCAACGGACCGTTTCCGTCTTGCGTTACGACGTTTCACCTGGTC
ACCGGCTAATCCAGAAGCCAAGGCAAAGCTGTTGATCCCTGCGAAGAACCTTTCGGATAATGCTCGCAGCCTCGATTCCG
GTTCCACTGAACCCGTCGAGATCGCCGTGGGCACTGGCGAAAACATCGGTGCTGAAGGCCTGTTTGGTATTCACATCGAT
AACCGCCAGACCACCACGCGCATGCTCGACGCGGACTTCCCGAATGTCAGCCCGCTGCTGCCCAAGGTGCATAACGCAAT
GGCCAGCGTGGAAATTTCTGCGCTTTCCGACGCCATCCGCCGCGTAGCCCTAGTTGCTGAGCGCAACGCTCAGATCCGGA
TGCAGTTCACCCGTGACGAGGTTATTCTCTCTGCTGGTGGTTCGGATGCAGGTCACGCCGAGGAATCCGTGCCTTGTGCT
TTTACCGGCGACCAAGAATTTTTGATCGCTTTCAACTCGGCATATCTGCGTGATGGCTTGAGCGTGATCCGGACTAGCCG
TGTAGTCTTCGGTTTCACGGAGCCATCTCGCCCCGCAATTATGATTCCGGAGCCCGAGACGCTGCCGGAAGCAAGCGCTG
ATGGAACGTACCCTACGCCAGATACCGAATTCACCTACCTGCTCATGCCAGTTCGACTGCCAGGATAA

Upstream 100 bases:

>100_bases
AGGTACGGTTAGTTTTTGTAATTTCAAGAAGTCTTCCGATCCGCAGGCTCCTCCTCATGGCAGCGCCTACGTGATCAATC
ACATCGAGGAGCCCTCAAGC

Downstream 100 bases:

>100_bases
AAACACTCTCTGACTAGTGCAAAGGACGTCGCCCCACCGTGTACATTCGTGAGCTTTCGCTTCGAGATTTCCGCTCGTGG
CCAGAGTGCACTGTCACTTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MDAHAVSFRVEKDDLSGAVSWVARNLPTKPTQPVLRAVVITADDEGLEFAGFDYEVSTKVHINADVRQPGRIAVAGKLMS
DIIGTLPNKTIEVFVEGTQVQVVCGSSRFELPLIPLDEYPPLPQLPAVTGAIDPNLFTDAVLQVAAAAGRDETLPMLTGI
HMEIDGENVTLTATDRFRLALRRFTWSPANPEAKAKLLIPAKNLSDNARSLDSGSTEPVEIAVGTGENIGAEGLFGIHID
NRQTTTRMLDADFPNVSPLLPKVHNAMASVEISALSDAIRRVALVAERNAQIRMQFTRDEVILSAGGSDAGHAEESVPCA
FTGDQEFLIAFNSAYLRDGLSVIRTSRVVFGFTEPSRPAIMIPEPETLPEASADGTYPTPDTEFTYLLMPVRLPG

Sequences:

>Translated_395_residues
MDAHAVSFRVEKDDLSGAVSWVARNLPTKPTQPVLRAVVITADDEGLEFAGFDYEVSTKVHINADVRQPGRIAVAGKLMS
DIIGTLPNKTIEVFVEGTQVQVVCGSSRFELPLIPLDEYPPLPQLPAVTGAIDPNLFTDAVLQVAAAAGRDETLPMLTGI
HMEIDGENVTLTATDRFRLALRRFTWSPANPEAKAKLLIPAKNLSDNARSLDSGSTEPVEIAVGTGENIGAEGLFGIHID
NRQTTTRMLDADFPNVSPLLPKVHNAMASVEISALSDAIRRVALVAERNAQIRMQFTRDEVILSAGGSDAGHAEESVPCA
FTGDQEFLIAFNSAYLRDGLSVIRTSRVVFGFTEPSRPAIMIPEPETLPEASADGTYPTPDTEFTYLLMPVRLPG
>Mature_395_residues
MDAHAVSFRVEKDDLSGAVSWVARNLPTKPTQPVLRAVVITADDEGLEFAGFDYEVSTKVHINADVRQPGRIAVAGKLMS
DIIGTLPNKTIEVFVEGTQVQVVCGSSRFELPLIPLDEYPPLPQLPAVTGAIDPNLFTDAVLQVAAAAGRDETLPMLTGI
HMEIDGENVTLTATDRFRLALRRFTWSPANPEAKAKLLIPAKNLSDNARSLDSGSTEPVEIAVGTGENIGAEGLFGIHID
NRQTTTRMLDADFPNVSPLLPKVHNAMASVEISALSDAIRRVALVAERNAQIRMQFTRDEVILSAGGSDAGHAEESVPCA
FTGDQEFLIAFNSAYLRDGLSVIRTSRVVFGFTEPSRPAIMIPEPETLPEASADGTYPTPDTEFTYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=392, Percent_Identity=24.234693877551, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42601; Mature: 42601

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAHAVSFRVEKDDLSGAVSWVARNLPTKPTQPVLRAVVITADDEGLEFAGFDYEVSTKV
CCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEECCCEEEEEEE
HINADVRQPGRIAVAGKLMSDIIGTLPNKTIEVFVEGTQVQVVCGSSRFELPLIPLDEYP
EEEECCCCCCEEEEHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCEEEEEECCCCC
PLPQLPAVTGAIDPNLFTDAVLQVAAAAGRDETLPMLTGIHMEIDGENVTLTATDRFRLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECHHHHHH
LRRFTWSPANPEAKAKLLIPAKNLSDNARSLDSGSTEPVEIAVGTGENIGAEGLFGIHID
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
NRQTTTRMLDADFPNVSPLLPKVHNAMASVEISALSDAIRRVALVAERNAQIRMQFTRDE
CCCCEEEEEECCCCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCE
VILSAGGSDAGHAEESVPCAFTGDQEFLIAFNSAYLRDGLSVIRTSRVVFGFTEPSRPAI
EEEECCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHCCHHHHEECEEEEEECCCCCCEE
MIPEPETLPEASADGTYPTPDTEFTYLLMPVRLPG
ECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MDAHAVSFRVEKDDLSGAVSWVARNLPTKPTQPVLRAVVITADDEGLEFAGFDYEVSTKV
CCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCEEECCCEEEEEEE
HINADVRQPGRIAVAGKLMSDIIGTLPNKTIEVFVEGTQVQVVCGSSRFELPLIPLDEYP
EEEECCCCCCEEEEHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCEEEEEECCCCC
PLPQLPAVTGAIDPNLFTDAVLQVAAAAGRDETLPMLTGIHMEIDGENVTLTATDRFRLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECHHHHHH
LRRFTWSPANPEAKAKLLIPAKNLSDNARSLDSGSTEPVEIAVGTGENIGAEGLFGIHID
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
NRQTTTRMLDADFPNVSPLLPKVHNAMASVEISALSDAIRRVALVAERNAQIRMQFTRDE
CCCCEEEEEECCCCCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCE
VILSAGGSDAGHAEESVPCAFTGDQEFLIAFNSAYLRDGLSVIRTSRVVFGFTEPSRPAI
EEEECCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHCCHHHHEECEEEEEECCCCCCEE
MIPEPETLPEASADGTYPTPDTEFTYLLMPVRLPG
ECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]