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The map label for this gene is priA [H]

Identifier: 37681196

GI number: 37681196

Start: 3092601

End: 3094820

Strand: Direct

Name: priA [H]

Synonym: VV3012

Alternate gene names: 37681196

Gene position: 3092601-3094820 (Clockwise)

Preceding gene: 37681193

Following gene: 37681197

Centisome position: 92.19

GC content: 48.65

Gene sequence:

>2220_bases
ATGAAACACCACGTTATGCGTCCATCCATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGT
TCCTGGACACCTTTTTCCGATCATTGGTGGCCGCGTGAGTGTGCCATTTGGACGACAAACCTTGATTGGCATTGTCACCG
CTTTGGTTCAAGAATCAGACTTTCCACGCGAGCAACTTAAACCCATTAAAGCGGTGTTGGATCATCGTCCTATTTGGCCC
GATTCCGTCTACAGTTTATTACTTTGGTGTAGCCAGTTTTACCAATATCCCTTAGGTGAAACACTGCATAACGCCCTCCC
TGCAGCACTAAGAAAAGGCAAAGCGGCTGACTTTGCCACTCTACAAGAATGGCAACTGACCGAGTTAGGCAAAAACCAGT
TGATGCAAGGGTTAAACAGTCGTGCGATAAAGCAGGCTAAAGTATTAAATATGCTTGCTAATGGCCCTCTGCCTCACCAA
ACCTTTTTGGATGAGGAAGTGGCATCCACAACCTTACGCACCTTGGAAGAAAAAGGCTGGATCAGCTGTGAAGAAAAAAC
GCCACAGTTAACTCGTTGGGGTAACCCGATTGAACATCCCGTCGATAAACCAAAACTCAACCAAGAGCAAGCCATTGCCA
TTGCGACCGTTAACAGCCAGACAGACTTTGGCTGTTATCTGTTGGAAGGGGTCACAGGTTCAGGCAAAACCGAAGTTTAT
CTCAACTTGATCAAACCCATTTTGGAACAAGGTAAACAGGCTCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAAC
CATCAATCGTTTTCGTCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACGGAACGTCTCAACG
CTTGGCTTTCCGCACGAGACAAAGCGGCGGGCATTGTCATTGGTACACGCTCAGCACTACTGATCCCATTTGCCCAGCTA
GGGATCATCATTGTCGATGAAGAGCACGACGCTTCGTACAAACAGCAAGATAGCTTGCGTTACCATGCTCGCGATGTGGC
TGTGATGCGAGCGGCAAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCCACGCCCGCGTTAGAAACCTTACACAACGCAC
TTACGGGCAAATATCATCATCTCTCGCTAACCCAACGAGCAGGAGTGGCCGTGCCAACCACCAACAAAGTGCTGGATGTC
AAAGGGCTCTATCTGGAAAGCGGCCTATCAGCGCCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGT
GATGCTGTTTCTCAACCGTCGAGGCTTCTCCCCTGCGTTAATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGCT
GCGATGCCTATTACACTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGTTCTCAACAACCTATTATCCAT
CAATGCCAAGGTTGTGGTTCGACACAACTGGTGACGGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTGACGGCCCT
GTTTCCCGATTACAAAGCGATTCGCATCGACCGCGATAGCACACGACGCAAAGGCAGCCTCGAAAGCGCTTTAGAAGCCA
TTCGCAAAGGGGAATACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGGCATCATTTCCCAGACGTGACGTTAGTG
GCACTGTTGGATGTGGATGGGTCACTGTACAGCAGTGATTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGT
CGCAGGACGAGCAGGGCGTGCTAGCAAACCTGGCGAAGTGATATTACAAACCCACCATCCAGAACACACCTTGCTCAAAG
CTCTGTTGACCAAGGATTACCGGCATTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGT
TTTCTAACGCTATTTCGCGCAGAAGCCAATCACAGCGAAATCGTCGAAACATTCTTACGTCAAGTGCGTATGACTCTCGA
GTCTCATCCCTTGTTTGATCACACTTGTCTCGTACTCGGTCCAACACCCGCTCCACTGGCCAAACGTGCGGGCAAATACC
GCTGGCAGCTTCTGCTTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTG
TTACCAGATACGAAAAAAGTACGTTGGAATTTGGATATTGAGCCGCAGGATCTGAGTTAG

Upstream 100 bases:

>100_bases
TCCGCTAGTTTTTTATACAGTTTTTCTCTCTCTGTTCGTGCATGCCCTTTTCTCGTATTTCTACCTCGCTTCCATTAGAC
TGTAAGTCATTATTTTTGCC

Downstream 100 bases:

>100_bases
CAAACGTTTACCACCTCACTCTAACGTTGAAAATTGCAACAATTTGCTTCTTTACGTGATGACCATCACGAATGAAATGC
AGATTTTGTTAATTCGCCCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MKHHVMRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPIKAVLDHRPIWP
DSVYSLLLWCSQFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQ
TFLDEEVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVY
LNLIKPILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQL
GIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDV
KGLYLESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIH
QCQGCGSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLV
ALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFS
FLTLFRAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDL
LPDTKKVRWNLDIEPQDLS

Sequences:

>Translated_739_residues
MKHHVMRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPIKAVLDHRPIWP
DSVYSLLLWCSQFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQ
TFLDEEVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVY
LNLIKPILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQL
GIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDV
KGLYLESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIH
QCQGCGSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLV
ALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFS
FLTLFRAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDL
LPDTKKVRWNLDIEPQDLS
>Mature_739_residues
MKHHVMRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPIKAVLDHRPIWP
DSVYSLLLWCSQFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQ
TFLDEEVASTTLRTLEEKGWISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVY
LNLIKPILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQL
GIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDV
KGLYLESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIH
QCQGCGSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLV
ALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFS
FLTLFRAEANHSEIVETFLRQVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDL
LPDTKKVRWNLDIEPQDLS

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=728, Percent_Identity=53.9835164835165, Blast_Score=785, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82669; Mature: 82669

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHHVMRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESD
CCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHCCC
FPREQLKPIKAVLDHRPIWPDSVYSLLLWCSQFYQYPLGETLHNALPAALRKGKAADFAT
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHH
LQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQTFLDEEVASTTLRTLEEKGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVY
EEECCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHH
LNLIKPILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARD
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCC
KAAGIVIGTRSALLIPFAQLGIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVL
CCCEEEEECCCHHHEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYLESGLSAPLIAEMRRH
CCCCHHHHHHHHHHCCCEEEEEEHHHCCEECCCCCCEEEECCEEECCCCCCHHHHHHHHH
LKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIH
HCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEHHHHCCCEEEEECCCCCCHHH
QCQGCGSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQ
HHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEE
ILIGTQMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEV
EEEEHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLFRAEANHSEIVETFLR
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
QVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDL
HHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LPDTKKVRWNLDIEPQDLS
CCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKHHVMRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESD
CCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHCCC
FPREQLKPIKAVLDHRPIWPDSVYSLLLWCSQFYQYPLGETLHNALPAALRKGKAADFAT
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHH
LQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQTFLDEEVASTTLRTLEEKGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
ISCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVY
EEECCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHH
LNLIKPILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARD
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCC
KAAGIVIGTRSALLIPFAQLGIIIVDEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVL
CCCEEEEECCCHHHEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GSATPALETLHNALTGKYHHLSLTQRAGVAVPTTNKVLDVKGLYLESGLSAPLIAEMRRH
CCCCHHHHHHHHHHCCCEEEEEEHHHCCEECCCCCCEEEECCEEECCCCCCHHHHHHHHH
LKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIH
HCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHCCHHEEHHHHCCCEEEEECCCCCCHHH
QCQGCGSTQLVTVGVGTEQLENQLTALFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQ
HHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEE
ILIGTQMLAKGHHFPDVTLVALLDVDGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEV
EEEEHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLFRAEANHSEIVETFLR
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
QVRMTLESHPLFDHTCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDL
HHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEE
LPDTKKVRWNLDIEPQDLS
CCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]