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The map label for this gene is pyrB

Identifier: 37681104

GI number: 37681104

Start: 2979830

End: 2980759

Strand: Direct

Name: pyrB

Synonym: VV2920

Alternate gene names: 37681104

Gene position: 2979830-2980759 (Clockwise)

Preceding gene: 37681103

Following gene: 37681105

Centisome position: 88.83

GC content: 50.11

Gene sequence:

>930_bases
ATGACAAACTCGCTTTATAAGAAGCACATTATCTCCATTCCAGAGCTTAGCCGCGCTGAGCTGGAATTGATTGTCAAAAC
GGCAGGTCAGTTAAAAGCAGAGCCAAACCCAGAGCTGATAAAGAACAAGGTCGTCGCGAGTTGTTTCTTCGAGCCTTCCA
CCCGTACTCGCCTGTCGTTTGAAACGGCCATCCAACGCATCGGTGGCGATGTCATTGGGTTTGACGATGGTGGTAACACC
TCACTGGCGAAAAAAGGCGAAACGCTGTCTGACTCGGTACAAGTCATCTCGAACTATGTGGATGCTTTTGTCATGCGTCA
CCCACAAGAAGGCGCTGCGCGTCTGGCTTCTGAATTTTCTAACGGTGTTCCGGTGATCAACGCCGGTGATGGCGCTAACC
AGCATCCAACTCAAACGCTGCTTGATCTGTTCACCATCTCCGAAACGCAAGATCGTTTGGACAACTTGAATGTGGCGTTT
GTCGGTGACTTAAAATACGGCCGCACCGTACACTCGTTGACGCAAGCCTTGGCAAAATTCAACAACATCCGCTTCTTCTT
TGTTGCGCCAGACGCATTGGCGATGCCAGATTACATTCTTGAAGATCTGGATGAAGCTGGCATTAGCTACAGTCTGCACA
CAGACATGGAAACCGTGATCCCTGAGCTGGATATTCTCTACATGACGCGCGTACAAAAAGAACGTTTCGATGAATCGGAA
TACGCGCACATTAAATCCGCCTACATCCTCACCGCAGCGCTATTGGAAGGCGCACGTGAGAACCTCAAAGTGCTGCATCC
ACTGCCACGTGTGGATGAGATCACCACTGACGTCGACAAAACACCGCACGCTTACTACTTCCAGCAAGCAGGCAATGGCG
TTTACGCTCGTGAAGCCTTGTTGGCACTGGTACTGAACGAATCACTGTAA

Upstream 100 bases:

>100_bases
GTTTCTTCTGCCATTGTATTTGTTCAACCAATAGAATTTTGAAGCCTCCCGTCATGGGGGGCTTTTTTATGCCTGAAACA
TTCAGATAGGGAAGACGATT

Downstream 100 bases:

>100_bases
GTAGGAGAATCAAGATGAACAAAGAGACAAAATTGCAGGTTGAAGCGATTAAAAACGGTACAGTGATCGACCACATTCCA
GCGCAAGTGGGCATCAAGGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDDGGNT
SLAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFTISETQDRLDNLNVAF
VGDLKYGRTVHSLTQALAKFNNIRFFFVAPDALAMPDYILEDLDEAGISYSLHTDMETVIPELDILYMTRVQKERFDESE
YAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDKTPHAYYFQQAGNGVYAREALLALVLNESL

Sequences:

>Translated_309_residues
MTNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDDGGNT
SLAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFTISETQDRLDNLNVAF
VGDLKYGRTVHSLTQALAKFNNIRFFFVAPDALAMPDYILEDLDEAGISYSLHTDMETVIPELDILYMTRVQKERFDESE
YAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDKTPHAYYFQQAGNGVYAREALLALVLNESL
>Mature_308_residues
TNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGDVIGFDDGGNTS
LAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTLLDLFTISETQDRLDNLNVAFV
GDLKYGRTVHSLTQALAKFNNIRFFFVAPDALAMPDYILEDLDEAGISYSLHTDMETVIPELDILYMTRVQKERFDESEY
AHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDKTPHAYYFQQAGNGVYAREALLALVLNESL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=42.7652733118971, Blast_Score=251, Evalue=4e-67,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=72.168284789644, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1786469, Length=340, Percent_Identity=24.7058823529412, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=39.0092879256966, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=41.1003236245955, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6322373, Length=331, Percent_Identity=25.3776435045317, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=44.0514469453376, Blast_Score=254, Evalue=6e-68,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34266; Mature: 34135

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSF
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ETAIQRIGGDVIGFDDGGNTSLAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPVINAGDGANQHPTQTLLDLFTISETQDRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NNIRFFFVAPDALAMPDYILEDLDEAGISYSLHTDMETVIPELDILYMTRVQKERFDESE
CCEEEEEECCCHHHCHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDKTPHAYYFQQAGNGVYAREAL
HHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHCCCCCCCCEEHHHCCCCHHHHHHH
LALVLNESL
HHHHHHCCC
>Mature Secondary Structure 
TNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSF
CCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
ETAIQRIGGDVIGFDDGGNTSLAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFS
HHHHHHHCCCEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NGVPVINAGDGANQHPTQTLLDLFTISETQDRLDNLNVAFVGDLKYGRTVHSLTQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NNIRFFFVAPDALAMPDYILEDLDEAGISYSLHTDMETVIPELDILYMTRVQKERFDESE
CCEEEEEECCCHHHCHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHHHHHHHHCCCHH
YAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDKTPHAYYFQQAGNGVYAREAL
HHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHCCCCCCCCEEHHHCCCCHHHHHHH
LALVLNESL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA