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The map label for this gene is tapB [H]

Identifier: 37680963

GI number: 37680963

Start: 2834105

End: 2835793

Strand: Direct

Name: tapB [H]

Synonym: VV2779

Alternate gene names: 37680963

Gene position: 2834105-2835793 (Clockwise)

Preceding gene: 37680962

Following gene: 37680964

Centisome position: 84.49

GC content: 48.73

Gene sequence:

>1689_bases
ATGCACAGCAATCTTGCCACTATTCTGCGTCAGGCCAAACAACTTAGCCTAACGCAAGAGCAAGCGTGTCGAGAGACAAT
ACAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGCTTTTTCCAGCCTGATGAACTAACCGAACAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTTGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATTTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCCTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GAATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Upstream 100 bases:

>100_bases
CACGACATTCCGACAACACTGCTAACGGAAGAGTTAAAGAGTTGCAAAGCTACCACAGCGCAACAACAAACTCCATCATC
ATAATAAATACAATATCTAA

Downstream 100 bases:

>100_bases
TTTTTATAGTTTGTTTTTTCGATACTCATCGCACTGAAGGAAGCGATATGAAGCTTAAAACCCATTCACAAATCAAAAAC
TATCAATGGAAAGGGATTAA

Product: type IV pilus (Tfp) assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Sequences:

>Translated_562_residues
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL
>Mature_562_residues
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=485, Percent_Identity=42.4742268041237, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=48.5365853658537, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=31.2056737588652, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62453; Mature: 62453

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLP
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
RTDLNQYDYQELCNQLGLRELITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQ
CCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCE
VEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELANLVEVSDDEVESIEDLSQDE
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
SPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHH
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKL
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GYNPQQKQLYLDALRKPQGMILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINL
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCCEECCCCCEEEEC
PGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEC
TNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHH
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKD
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHH
SGLEKLKQGITSLEELQRVLYL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLP
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCC
RTDLNQYDYQELCNQLGLRELITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQ
CCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCE
VEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELANLVEVSDDEVESIEDLSQDE
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
SPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECEEEEEECCCHHHHHHHHHHHHH
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKL
HHHHHHHHHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GYNPQQKQLYLDALRKPQGMILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINL
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCCEECCCCCEEEEC
PGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEEC
TNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHH
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKD
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEHH
SGLEKLKQGITSLEELQRVLYL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]