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The map label for this gene is maiA [H]

Identifier: 37679682

GI number: 37679682

Start: 1532046

End: 1532714

Strand: Direct

Name: maiA [H]

Synonym: VV1498

Alternate gene names: 37679682

Gene position: 1532046-1532714 (Clockwise)

Preceding gene: 37679681

Following gene: 37679683

Centisome position: 45.67

GC content: 49.03

Gene sequence:

>669_bases
ATGGAGACAAGTCTTAAACTCTATGGTTATTGGCGTTCGTCTGCCGCCTATCGCGTGAGGATCTGCTTAAACCTGAAGCA
GCTCCGCTATGAAAACTACTCGATTCATTTAGTGAAAAATGGCGGAGAGCAGCATCTCGCGCATTACCATGCTCTGAACC
CCAATGAGCTGGTGCCCGTGTTGGTGGATGGGGATCTGGTACTCAATCAGTCGTTGGCTATCATTCAGTATCTCGATGAT
AACTATTCATCGACGCAAGTGATCCCGTCTATGGGGCCACTCAAGTATCAAGCGTTGGCGTTGGCGCAAGACATCGCGAT
TGATGTTCATCCTCTGAATAATCTTCGGGTCCTGCAGTATCTTGAAGGCAGCTTGGAGGTGGATGAAGAGCAAAAACGCT
TGTGGGTGCATCACTGGATCAACACAGGCTTTAAAGCGGTGGAAGAAAAACTCATGGCTCACCGTAAACACTATGGCGAG
TGTGTTTTTAGCGTCAGTTCTAGTCCAAGTATCGTCGATATATGTTTGGTCCCTCAGGTGTATAACGCGCTCCGTTTTGG
CGTAAACATGGCACCGTATCCCACCATCAATGCCGTCGTTGCCGCTTGCAATCAACTGCCCGCATTCGATCTCGCCAAAC
CCGAAAATCAGCCTGACGCTCAACTTTGA

Upstream 100 bases:

>100_bases
GGCGATTCTATTCGCTTAGAAATGTTTGATGCGCAAGGGCAATCTATTTTTGGTGCGATTGAACAACAAGTGAGTCGTTA
TCAGCATAAATGAGGTTGGT

Downstream 100 bases:

>100_bases
ATTTACAGGTGGCGGTGTGATTCCTTGTTGGTAGATGTGTCAATTCGGGACTACATTTACTTGCAAGGGATCACAATTAG
GGGGCGTTATGTTCCGTTCA

Product: glutathione S-transferase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENYSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWINTGFKAVEEKLMAHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMAPYPTINAVVAACNQLPAFDLAKPENQPDAQL

Sequences:

>Translated_222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENYSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWINTGFKAVEEKLMAHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMAPYPTINAVVAACNQLPAFDLAKPENQPDAQL
>Mature_222_residues
METSLKLYGYWRSSAAYRVRICLNLKQLRYENYSIHLVKNGGEQHLAHYHALNPNELVPVLVDGDLVLNQSLAIIQYLDD
NYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQYLEGSLEVDEEQKRLWVHHWINTGFKAVEEKLMAHRKHYGE
CVFSVSSSPSIVDICLVPQVYNALRFGVNMAPYPTINAVVAACNQLPAFDLAKPENQPDAQL

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=215, Percent_Identity=38.6046511627907, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI194440732, Length=214, Percent_Identity=32.2429906542056, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI22202622, Length=164, Percent_Identity=35.3658536585366, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556142, Length=215, Percent_Identity=39.0697674418605, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17510461, Length=214, Percent_Identity=37.3831775700935, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551302, Length=216, Percent_Identity=33.7962962962963, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645375, Length=215, Percent_Identity=40.4651162790698, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355859, Length=215, Percent_Identity=40.4651162790698, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553325, Length=215, Percent_Identity=40.4651162790698, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21355857, Length=222, Percent_Identity=32.8828828828829, Blast_Score=120, Evalue=9e-28,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 25101; Mature: 25101

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSLKLYGYWRSSAAYRVRICLNLKQLRYENYSIHLVKNGGEQHLAHYHALNPNELVPV
CCCCEEEEEEECCCCEEEEEEEEEHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCEEEE
LVDGDLVLNQSLAIIQYLDDNYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQY
EECCCEEECCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHEEEEECCCCEEEEEH
LEGSLEVDEEQKRLWVHHWINTGFKAVEEKLMAHRKHYGECVFSVSSSPSIVDICLVPQV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECHHH
YNALRFGVNMAPYPTINAVVAACNQLPAFDLAKPENQPDAQL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METSLKLYGYWRSSAAYRVRICLNLKQLRYENYSIHLVKNGGEQHLAHYHALNPNELVPV
CCCCEEEEEEECCCCEEEEEEEEEHHHHCCCCEEEEEEECCCHHHHHHHCCCCCCCEEEE
LVDGDLVLNQSLAIIQYLDDNYSSTQVIPSMGPLKYQALALAQDIAIDVHPLNNLRVLQY
EECCCEEECCCEEEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHEEEEECCCCEEEEEH
LEGSLEVDEEQKRLWVHHWINTGFKAVEEKLMAHRKHYGECVFSVSSSPSIVDICLVPQV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECHHH
YNALRFGVNMAPYPTINAVVAACNQLPAFDLAKPENQPDAQL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]