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The map label for this gene is cobS

Identifier: 37679659

GI number: 37679659

Start: 1512315

End: 1513082

Strand: Direct

Name: cobS

Synonym: VV1475

Alternate gene names: 37679659

Gene position: 1512315-1513082 (Clockwise)

Preceding gene: 37679658

Following gene: 37679660

Centisome position: 45.08

GC content: 50.39

Gene sequence:

>768_bases
ATGAAAACCTGGCAGTATCAGTACCAATTGTTTTGTTTGGCGGTGAGCTTTTTTAGCCGTTTGCCTGTGCCGAAATCAAC
ACCGTATAGTGATGAAAGAATGAATCAGGCTGGGCGTTACTTTGCTTTAGTCGGTACATTGCTTGGCTTACTGTGCGTAC
TGGTTTATGCGTTTGCATCGCTGTTTTTCCCTTATCAGGTAGCGATTGTCCTGATGATGGCGTTTAGCTTACTGCTGACA
GGCGCCTTCCATGAAGATGGACTCACTGACATGGCGGATGGCATTGGTGGGGGGATGACGCTCGACAAACGCCTCACCAT
CATGAAGGACAGTCGTATCGGAACCTATGGCAGCGCCACATTAACCATGGCGCTGATAGGTAAGTTCGTTTTTCTCACCA
CGTTGGCACGTCAACCTGATTTTGGCTTGATGATTGTTGTCGCCTACACCTTGAGCCGAGCCGTTGCCGCCACACTGATT
TACGACATGCCCTATGTCTCTGACAGCGATACTAGTAAAAGCAAGCCATTGGCGAATGCACAATCCTCCACGGAGCTGGC
GATACTGATCCTCACTGGGGTATTAGCGGCAATCAGCTTAGGGCTGGGGGTCGGTTTGCTATTGATTTTATTTGCGATCC
TCTTCCGCTGGGCATTCAAACGTTGGCTGCTGGCGCGTTTGGGCGGGTTTACCGGAGATTGCTTGGGCGGCGCTCAACAA
TTGATGGAACTCGGCATCTATTTGGTTCTGATTGCGGTGGTGCAGTGA

Upstream 100 bases:

>100_bases
GAAGGAACAGGAGCTGCACTGGCGTACCCACTATTAAAAGCGGCGGCGCAGTTTTACAACAATATGGCAAGCTTCGAAAG
CGCAGGAGTAACGGTTTGAT

Downstream 100 bases:

>100_bases
CGATGAGTTATTCAACCAACAAAGCGCTGGTGTTGGGTGGGGCTCGCTCGGGTAAATCTCGTTTTGCTGAACAGCTTGTG
CGGCAATGGTCTCAACAACA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKTWQYQYQLFCLAVSFFSRLPVPKSTPYSDERMNQAGRYFALVGTLLGLLCVLVYAFASLFFPYQVAIVLMMAFSLLLT
GAFHEDGLTDMADGIGGGMTLDKRLTIMKDSRIGTYGSATLTMALIGKFVFLTTLARQPDFGLMIVVAYTLSRAVAATLI
YDMPYVSDSDTSKSKPLANAQSSTELAILILTGVLAAISLGLGVGLLLILFAILFRWAFKRWLLARLGGFTGDCLGGAQQ
LMELGIYLVLIAVVQ

Sequences:

>Translated_255_residues
MKTWQYQYQLFCLAVSFFSRLPVPKSTPYSDERMNQAGRYFALVGTLLGLLCVLVYAFASLFFPYQVAIVLMMAFSLLLT
GAFHEDGLTDMADGIGGGMTLDKRLTIMKDSRIGTYGSATLTMALIGKFVFLTTLARQPDFGLMIVVAYTLSRAVAATLI
YDMPYVSDSDTSKSKPLANAQSSTELAILILTGVLAAISLGLGVGLLLILFAILFRWAFKRWLLARLGGFTGDCLGGAQQ
LMELGIYLVLIAVVQ
>Mature_255_residues
MKTWQYQYQLFCLAVSFFSRLPVPKSTPYSDERMNQAGRYFALVGTLLGLLCVLVYAFASLFFPYQVAIVLMMAFSLLLT
GAFHEDGLTDMADGIGGGMTLDKRLTIMKDSRIGTYGSATLTMALIGKFVFLTTLARQPDFGLMIVVAYTLSRAVAATLI
YDMPYVSDSDTSKSKPLANAQSSTELAILILTGVLAAISLGLGVGLLLILFAILFRWAFKRWLLARLGGFTGDCLGGAQQ
LMELGIYLVLIAVVQ

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=250, Percent_Identity=32, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_VIBVU (Q8D922)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761602.1
- GeneID:   1179632
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2787
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   PRK00235
- BioCyc:   VVUL216895:VV1_2787-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27797; Mature: 27797

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x188097a0)-; HASH(0x1835bec8)-; HASH(0x18812124)-; HASH(0x187d430c)-; HASH(0x15c9266c)-; HASH(0x18140408)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCH
>Mature Secondary Structure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HHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA