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The map label for this gene is pldA [H]

Identifier: 37679650

GI number: 37679650

Start: 1503963

End: 1504730

Strand: Direct

Name: pldA [H]

Synonym: VV1466

Alternate gene names: 37679650

Gene position: 1503963-1504730 (Clockwise)

Preceding gene: 37679649

Following gene: 37679651

Centisome position: 44.83

GC content: 45.96

Gene sequence:

>768_bases
ATGAAGTTTAAAGCGCTCGCATTGCTCTTAACCGCCCCTTTGTTTGCTCAGGCGAAAAGCCTCAATACCATTTCCAGTTA
TGAAGATAACTATGCGCTTGGCACTTATACCTCCGATATCAATGATGAAGCCTATGAAGCCAGTGGTTCCGGCATGGATA
ATTTGCAGCACTTCGAAGTGAAGTTTCAGCTCTCGGTTTCGGTTCCCCTCTATCGATTTAATAAAGCCACTGCCGTCGTG
GGTTCCTATACGCAAAAATCCCTTTGGCAATTGGCCAATTCTGATATCTCTTCTCCCTTTAGAGAAACCAACTATAAGCC
CCAGCTGTTTATTGCTCATCAAAGCAATATGCTGATCTTTAACCATTTGGAGGCGGGCTATAAGCACGAGTCTAATGGTC
GTTCATCGGCGTTATCTCGTAGTTGGGATCGTCTCTACCTTGCTGCAGAGCGTTTGGATGGGCCGTTGGAGTACGGTGTG
CACCTTTGGTCTGTGGTGGGGGATACATCAGAAAACCGAGATATTGAAGATTACTACGCACCGTATGAAATTTGGATGAA
GCTTTACAGCGCGGCAGGCATATTTAATGCTCGAGGATTCTACAATTGGGATGACAGCCGTGGTGGCGTAGAAGTGGGCT
ATACCTTTTACTTTAATGATCTGATTGGCATCTACATTCAGGCGTACCATGGTTATGGTGAAACTCTGATCGATTATGAC
CACAATCAAACTCGGATCGGTGCCGGTTTCAAGCTGGTACGCTGGTAA

Upstream 100 bases:

>100_bases
TGCCGCTTTATGGCAACTTGCTCAAGCCCTTGTTTAGTGTTTGAAAGAGAAATAACCGTTCTCGAATAAATGGCTAAGCA
TCTATCACTGAAGGAACACA

Downstream 100 bases:

>100_bases
GTAAAAACGATGCTCTCCCTCTCTTAAATGGGAGGGTATTTGTTTTAAACGCAAAATATTAATCAATCCGACGCATATCG
TTAAAGGGTCTGAGTGACGA

Product: outer membrane phospholipase A

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKFKALALLLTAPLFAQAKSLNTISSYEDNYALGTYTSDINDEAYEASGSGMDNLQHFEVKFQLSVSVPLYRFNKATAVV
GSYTQKSLWQLANSDISSPFRETNYKPQLFIAHQSNMLIFNHLEAGYKHESNGRSSALSRSWDRLYLAAERLDGPLEYGV
HLWSVVGDTSENRDIEDYYAPYEIWMKLYSAAGIFNARGFYNWDDSRGGVEVGYTFYFNDLIGIYIQAYHGYGETLIDYD
HNQTRIGAGFKLVRW

Sequences:

>Translated_255_residues
MKFKALALLLTAPLFAQAKSLNTISSYEDNYALGTYTSDINDEAYEASGSGMDNLQHFEVKFQLSVSVPLYRFNKATAVV
GSYTQKSLWQLANSDISSPFRETNYKPQLFIAHQSNMLIFNHLEAGYKHESNGRSSALSRSWDRLYLAAERLDGPLEYGV
HLWSVVGDTSENRDIEDYYAPYEIWMKLYSAAGIFNARGFYNWDDSRGGVEVGYTFYFNDLIGIYIQAYHGYGETLIDYD
HNQTRIGAGFKLVRW
>Mature_255_residues
MKFKALALLLTAPLFAQAKSLNTISSYEDNYALGTYTSDINDEAYEASGSGMDNLQHFEVKFQLSVSVPLYRFNKATAVV
GSYTQKSLWQLANSDISSPFRETNYKPQLFIAHQSNMLIFNHLEAGYKHESNGRSSALSRSWDRLYLAAERLDGPLEYGV
HLWSVVGDTSENRDIEDYYAPYEIWMKLYSAAGIFNARGFYNWDDSRGGVEVGYTFYFNDLIGIYIQAYHGYGETLIDYD
HNQTRIGAGFKLVRW

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=236, Percent_Identity=43.6440677966102, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 29095; Mature: 29095

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKALALLLTAPLFAQAKSLNTISSYEDNYALGTYTSDINDEAYEASGSGMDNLQHFEV
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCHHCCCCCCCCCCEEEEE
KFQLSVSVPLYRFNKATAVVGSYTQKSLWQLANSDISSPFRETNYKPQLFIAHQSNMLIF
EEEEEEECCEEECCCCEEEECCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCEEEE
NHLEAGYKHESNGRSSALSRSWDRLYLAAERLDGPLEYGVHLWSVVGDTSENRDIEDYYA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCHHHHHC
PYEIWMKLYSAAGIFNARGFYNWDDSRGGVEVGYTFYFNDLIGIYIQAYHGYGETLIDYD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHEEEEEEECCCCCEEEECC
HNQTRIGAGFKLVRW
CCCEEECCCEEEECC
>Mature Secondary Structure
MKFKALALLLTAPLFAQAKSLNTISSYEDNYALGTYTSDINDEAYEASGSGMDNLQHFEV
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCCHHCCCCCCCCCCEEEEE
KFQLSVSVPLYRFNKATAVVGSYTQKSLWQLANSDISSPFRETNYKPQLFIAHQSNMLIF
EEEEEEECCEEECCCCEEEECCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCEEEE
NHLEAGYKHESNGRSSALSRSWDRLYLAAERLDGPLEYGVHLWSVVGDTSENRDIEDYYA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCHHHHHC
PYEIWMKLYSAAGIFNARGFYNWDDSRGGVEVGYTFYFNDLIGIYIQAYHGYGETLIDYD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHEEEEEEECCCCCEEEECC
HNQTRIGAGFKLVRW
CCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8300539 [H]