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The map label for this gene is rsuA

Identifier: 37679610

GI number: 37679610

Start: 1452521

End: 1453222

Strand: Direct

Name: rsuA

Synonym: VV1426

Alternate gene names: 37679610

Gene position: 1452521-1453222 (Clockwise)

Preceding gene: 37679601

Following gene: 161486627

Centisome position: 43.3

GC content: 47.01

Gene sequence:

>702_bases
ATGCGTTTAGATAAATTTCTTTGCGATGCGCTTGGCGCGACGCGAAAAGAGGCCACTAAGATCCTAAAAAGTGGTGAAGT
GACCGTTAACGAACAAGTGCAAAAAAGCGGTGCGGTAAAAGTAACGGAAGAGTGTGTGGTTGAATGGCAAGGGCGCGAGC
TGGCTATGCAAGGACCGCGTTACATTATGCTTTACAAGCCTGAAGGTTTTGTCTGCTCGCATGAAGATGGTTTCAACCAC
ACTGCGTTTGTTTTATTAGACGAAGTAAAAATGGATGAGCTTCATTTTGCAGGTCGTTTGGATGTGGATACCACGGGCTT
GGTGTTGATCACCGACGACGGTAAGTGGTCACACCGTATTACTTCGCCTAAGCATAAATGCGACAAAACTTACCGTGTTT
GGTTGGCGGACCCGATTGGCAGTGATTATGCAGAAAAACTGGCCGAAGGCGTACAATTGCGTAACGAAAAGGAGCTAACG
CTTCCAGCGCAGATGACCATCGTCAATGCGGATGAAAACGAAGTGCTGCTGACTATCCATGAGGGCAAATACCATCAAGT
GAAACGCATGTTCGCGGCATTGGGTAACAAAGTTGAGCAATTACATCGTGAGCGTATTGGCGCGATCGAACTGGATGAAA
GTCTTGAGCCGGGAGAATACCGTTACCTCACTCAGGAAGAAATAGACTCTGTTTGGAAATGA

Upstream 100 bases:

>100_bases
GGCTTGATAAGGTCTGAGGGTGTACATGGAAACGATGAAATCACTCAACTATTCTTTGTGAGCTCGCTATAATACCCGAC
CTTAACCAGATGAGGTATTC

Downstream 100 bases:

>100_bases
TTTAGTTCCTCTACGGAACTAGTGTTTCTGCCTAATCTGTATCACAATAGTTCAGCCTGCTGACATTTCTTTAACCTCTT
CACGCAGGCTGCTATGGCAT

Product: 16S rRNA uridine-516 pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MRLDKFLCDALGATRKEATKILKSGEVTVNEQVQKSGAVKVTEECVVEWQGRELAMQGPRYIMLYKPEGFVCSHEDGFNH
TAFVLLDEVKMDELHFAGRLDVDTTGLVLITDDGKWSHRITSPKHKCDKTYRVWLADPIGSDYAEKLAEGVQLRNEKELT
LPAQMTIVNADENEVLLTIHEGKYHQVKRMFAALGNKVEQLHRERIGAIELDESLEPGEYRYLTQEEIDSVWK

Sequences:

>Translated_233_residues
MRLDKFLCDALGATRKEATKILKSGEVTVNEQVQKSGAVKVTEECVVEWQGRELAMQGPRYIMLYKPEGFVCSHEDGFNH
TAFVLLDEVKMDELHFAGRLDVDTTGLVLITDDGKWSHRITSPKHKCDKTYRVWLADPIGSDYAEKLAEGVQLRNEKELT
LPAQMTIVNADENEVLLTIHEGKYHQVKRMFAALGNKVEQLHRERIGAIELDESLEPGEYRYLTQEEIDSVWK
>Mature_233_residues
MRLDKFLCDALGATRKEATKILKSGEVTVNEQVQKSGAVKVTEECVVEWQGRELAMQGPRYIMLYKPEGFVCSHEDGFNH
TAFVLLDEVKMDELHFAGRLDVDTTGLVLITDDGKWSHRITSPKHKCDKTYRVWLADPIGSDYAEKLAEGVQLRNEKELT
LPAQMTIVNADENEVLLTIHEGKYHQVKRMFAALGNKVEQLHRERIGAIELDESLEPGEYRYLTQEEIDSVWK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=55.6034482758621, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=30.7359307359307, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSUA_VIBVU (Q8D8X2)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761653.1
- ProteinModelPortal:   Q8D8X2
- SMR:   Q8D8X2
- GeneID:   1179683
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2843
- HOGENOM:   HBG754478
- OMA:   GLHRWKI
- ProtClustDB:   CLSK874466
- BioCyc:   VVUL216895:VV1_2843-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26593; Mature: 26593

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCDALGATRKEATKILKSGEVTVNEQVQKSGAVKVTEECVVEWQGRELAMQGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCEECHHHHHCCCEEEEHHHHEEECCCEEEECCCE
YIMLYKPEGFVCSHEDGFNHTAFVLLDEVKMDELHFAGRLDVDTTGLVLITDDGKWSHRI
EEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKHKCDKTYRVWLADPIGSDYAEKLAEGVQLRNEKELTLPAQMTIVNADENEVLLTIH
CCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCEEEEEEECCCCEEEEEEE
EGKYHQVKRMFAALGNKVEQLHRERIGAIELDESLEPGEYRYLTQEEIDSVWK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECHHHHHHHCC
>Mature Secondary Structure
MRLDKFLCDALGATRKEATKILKSGEVTVNEQVQKSGAVKVTEECVVEWQGRELAMQGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCEECHHHHHCCCEEEEHHHHEEECCCEEEECCCE
YIMLYKPEGFVCSHEDGFNHTAFVLLDEVKMDELHFAGRLDVDTTGLVLITDDGKWSHRI
EEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCCCC
TSPKHKCDKTYRVWLADPIGSDYAEKLAEGVQLRNEKELTLPAQMTIVNADENEVLLTIH
CCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEECCEEEEEEECCCCEEEEEEE
EGKYHQVKRMFAALGNKVEQLHRERIGAIELDESLEPGEYRYLTQEEIDSVWK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA