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The map label for this gene is bcr [H]

Identifier: 161486627

GI number: 161486627

Start: 1453461

End: 1454663

Strand: Direct

Name: bcr [H]

Synonym: VV1427

Alternate gene names: 161486627

Gene position: 1453461-1454663 (Clockwise)

Preceding gene: 37679610

Following gene: 37679612

Centisome position: 43.33

GC content: 50.54

Gene sequence:

>1203_bases
ATGACGAATCCATCTCAAGCCGTTAGCCAATCACAAATTAGTCTCTTTTTGTTTGCTGTACTCGGTGCCATCGGTGCATT
GACACCCTTGGCCATCGACATGTATCTACCAGCGATGCCGACCATAGCACGCGATTTGGGCGTCGATGCCGGTGCGGTGC
AGTTTACTTTAACCGCTTATACCGCAGGTTTTGCGCTCGGCCAGCTGATTCATGGTCCACTGGCAGACAGTTTTGGTCGA
CGTCCGGTTTTATTGCTTGGGGTGCTCTTTTTTGGCCTAGCGGCGGTGGTCAGTGCCACTACCAATGGCATTGATGCGTT
AACGTATGTACGCACCGCGCAAGGCTTTGCGGGGGCCGCGGCTGCGGTGATCATTCAAGCCGTGGTTCGCGATATGTTTG
ATCGCGAGGACTTTGCGCGTGCGATGTCGTTCGTCACATTGGTGATCACTATTGCACCCCTTGTCGCGCCAATGATCGGT
GGGCACCTAGCCATTTGGTTTGGCTGGCGTTCAATTTTCTGGGTGCTGGCGTTTTTTGCCGTTATCGTGATCGCCCTAGT
TTGGTGGCAAATACCAGAAACGCTCAAGGTAGAAAACCGACAGCCATTGCGCTTCAAAACGACCATGCGTAACTACTTAA
AGTTATGCTGTAACAAAACAGCGATGGGGTTGATTCTGTCTGGCGCGTTCTCTTTCTCCGGAATGTTTGCTTTTTTAACC
GCAGGCTCGTTTGTCTATATTGATATTTACGGCATTTCACCCGATCAGTTTGGCTATTTGTTTGGCTTGAACATCGTTGC
GATGATCATCATGACCAGCCTAAATGGCCGAATGGTGAAGAAAGTGGGATCGCATTTCATGTTGCGTTTGGGGCTGACTG
TTCAATTGATCGCCGGTCTTGGTCTATTTGTCAGTTGGCTACTAGACCTCGGGCTATGGGGCACGGTACCATTCGTGGTT
CTATTTATCGGCACACTTTCTACCATCGGCAGTAACGCAATGGCGCTATTGCTTTCTGGCTACCCTAATATGGCGGGGAC
GGCGTCTTCTCTGGCGGGCACATTGCGTTTTGGCACTGGCTCTCTAGTTGGTGCTTTGGTTGCGATACTGCCATCAGGAG
TGACTTGGCCTATGGTGCTAGTGATGTCTGCGTGCTCTGTGCTTTCAGCACTGTTTTATTGGATTTTCGGACGAAAAGCA
TAA

Upstream 100 bases:

>100_bases
GACAACGGCCAACGTTTGGCAAACTGTTGTGGAACATGGGCAACGAGATTGTATTGCGACCGGAGTGTTGCGACGCAATC
GCAACCAAAACGGAGCATTA

Downstream 100 bases:

>100_bases
TGTCGAACTACACAAGTGAAATTCAGCGCTTAGTGAATACCGCACTCGCTGAGCTGGCTGCGGAACATCAATCGGGCAAA
TTGGTCGACGCACCGATTGC

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTNPSQAVSQSQISLFLFAVLGAIGALTPLAIDMYLPAMPTIARDLGVDAGAVQFTLTAYTAGFALGQLIHGPLADSFGR
RPVLLLGVLFFGLAAVVSATTNGIDALTYVRTAQGFAGAAAAVIIQAVVRDMFDREDFARAMSFVTLVITIAPLVAPMIG
GHLAIWFGWRSIFWVLAFFAVIVIALVWWQIPETLKVENRQPLRFKTTMRNYLKLCCNKTAMGLILSGAFSFSGMFAFLT
AGSFVYIDIYGISPDQFGYLFGLNIVAMIIMTSLNGRMVKKVGSHFMLRLGLTVQLIAGLGLFVSWLLDLGLWGTVPFVV
LFIGTLSTIGSNAMALLLSGYPNMAGTASSLAGTLRFGTGSLVGALVAILPSGVTWPMVLVMSACSVLSALFYWIFGRKA

Sequences:

>Translated_400_residues
MTNPSQAVSQSQISLFLFAVLGAIGALTPLAIDMYLPAMPTIARDLGVDAGAVQFTLTAYTAGFALGQLIHGPLADSFGR
RPVLLLGVLFFGLAAVVSATTNGIDALTYVRTAQGFAGAAAAVIIQAVVRDMFDREDFARAMSFVTLVITIAPLVAPMIG
GHLAIWFGWRSIFWVLAFFAVIVIALVWWQIPETLKVENRQPLRFKTTMRNYLKLCCNKTAMGLILSGAFSFSGMFAFLT
AGSFVYIDIYGISPDQFGYLFGLNIVAMIIMTSLNGRMVKKVGSHFMLRLGLTVQLIAGLGLFVSWLLDLGLWGTVPFVV
LFIGTLSTIGSNAMALLLSGYPNMAGTASSLAGTLRFGTGSLVGALVAILPSGVTWPMVLVMSACSVLSALFYWIFGRKA
>Mature_399_residues
TNPSQAVSQSQISLFLFAVLGAIGALTPLAIDMYLPAMPTIARDLGVDAGAVQFTLTAYTAGFALGQLIHGPLADSFGRR
PVLLLGVLFFGLAAVVSATTNGIDALTYVRTAQGFAGAAAAVIIQAVVRDMFDREDFARAMSFVTLVITIAPLVAPMIGG
HLAIWFGWRSIFWVLAFFAVIVIALVWWQIPETLKVENRQPLRFKTTMRNYLKLCCNKTAMGLILSGAFSFSGMFAFLTA
GSFVYIDIYGISPDQFGYLFGLNIVAMIIMTSLNGRMVKKVGSHFMLRLGLTVQLIAGLGLFVSWLLDLGLWGTVPFVVL
FIGTLSTIGSNAMALLLSGYPNMAGTASSLAGTLRFGTGSLVGALVAILPSGVTWPMVLVMSACSVLSALFYWIFGRKA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=49.3297587131367, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI48994889, Length=373, Percent_Identity=30.8310991957105, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082312, Length=351, Percent_Identity=28.7749287749288, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790794, Length=288, Percent_Identity=25.3472222222222, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790146, Length=238, Percent_Identity=25.6302521008403, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787065, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=29.7101449275362, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081738, Length=183, Percent_Identity=28.4153005464481, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI2367379, Length=174, Percent_Identity=26.4367816091954, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=181, Percent_Identity=24.3093922651934, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=172, Percent_Identity=27.3255813953488, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42764; Mature: 42633

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNPSQAVSQSQISLFLFAVLGAIGALTPLAIDMYLPAMPTIARDLGVDAGAVQFTLTAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHEEEHHHH
TAGFALGQLIHGPLADSFGRRPVLLLGVLFFGLAAVVSATTNGIDALTYVRTAQGFAGAA
HHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
AAVIIQAVVRDMFDREDFARAMSFVTLVITIAPLVAPMIGGHLAIWFGWRSIFWVLAFFA
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VIVIALVWWQIPETLKVENRQPLRFKTTMRNYLKLCCNKTAMGLILSGAFSFSGMFAFLT
HHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AGSFVYIDIYGISPDQFGYLFGLNIVAMIIMTSLNGRMVKKVGSHFMLRLGLTVQLIAGL
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLFVSWLLDLGLWGTVPFVVLFIGTLSTIGSNAMALLLSGYPNMAGTASSLAGTLRFGTG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCH
SLVGALVAILPSGVTWPMVLVMSACSVLSALFYWIFGRKA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]