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The map label for this gene is bioD

Identifier: 37679514

GI number: 37679514

Start: 1358889

End: 1359572

Strand: Direct

Name: bioD

Synonym: VV1330

Alternate gene names: 37679514

Gene position: 1358889-1359572 (Clockwise)

Preceding gene: 37679513

Following gene: 37679516

Centisome position: 40.51

GC content: 48.98

Gene sequence:

>684_bases
ATGATGAAAGCATTTTTCATTGCAGGTACGGATACCGATGTGGGCAAAACCGTGGCATCTAAAGCGATTCTTCACGCATT
AGCGGCCAAAGATCTACGTACTATTGGTTATAAACCAGTAGCGGCAGGCAGCGATAAAACGCCAGAAGGTTGGCGTAATT
CAGATGCCTTGCACCTGCAGAAAGCGGCGACAGTGGCCGTTGATTACGAGGACATCAATCCATATGCCCTCGAGTTGCCA
GCGTCTCCGCACATTGCGGCAAAACATGAAAAAGTAGAAATTGACTATGCTGTGCTGAGCCGCAAACTTGCGCAGCACAA
AGAGAATGCAGACGTCGTGCTTGTTGAAGGTGCGGGTGGTTGGCGAGTTCCAGTGTCAGACACTGACAGCCTGTCTACTT
GGGTTAAGCAAGAAGATCTGCCTGTTGTGTTGGTTGTTGGCATCAAATTGGGTTGCCTCAGCCACGCCTTACTGACCGCT
GAAGTGATCAAAGCCGATGGCCTTAATTTGGTCGGATGGGTTGCTAATCGAGTGAATCCTGGCACAGAGCACTACGCAGA
GATCATTGATATGTTGGAAGAGCGTTTGGAGGTTCCAAAGCTGGGCGAAATTCCTTATATCCCAAGCGCCAAGCGTAAAG
ACCTCGGTAAATACATCAATGCGGATGTGTTACTCGAGCTTTAA

Upstream 100 bases:

>100_bases
GACAAGCGTTAAAACGAGTGGAAAGAGAGTACCAGACATTTCAGAACCATCTAGGTCATGTACCGGCAAGTTATAAGGTT
TGTTTAGGGGTTATACAGTT

Downstream 100 bases:

>100_bases
AACATCAAAAGGGCTGCCGTTGGCAGCCCTTTCTTTATCAGGTCTTGCGCTTATTTGTCTTGAACTAAGCCCATAAGCGC
CTGTTGAGTATCCGAGATTT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MMKAFFIAGTDTDVGKTVASKAILHALAAKDLRTIGYKPVAAGSDKTPEGWRNSDALHLQKAATVAVDYEDINPYALELP
ASPHIAAKHEKVEIDYAVLSRKLAQHKENADVVLVEGAGGWRVPVSDTDSLSTWVKQEDLPVVLVVGIKLGCLSHALLTA
EVIKADGLNLVGWVANRVNPGTEHYAEIIDMLEERLEVPKLGEIPYIPSAKRKDLGKYINADVLLEL

Sequences:

>Translated_227_residues
MMKAFFIAGTDTDVGKTVASKAILHALAAKDLRTIGYKPVAAGSDKTPEGWRNSDALHLQKAATVAVDYEDINPYALELP
ASPHIAAKHEKVEIDYAVLSRKLAQHKENADVVLVEGAGGWRVPVSDTDSLSTWVKQEDLPVVLVVGIKLGCLSHALLTA
EVIKADGLNLVGWVANRVNPGTEHYAEIIDMLEERLEVPKLGEIPYIPSAKRKDLGKYINADVLLEL
>Mature_227_residues
MMKAFFIAGTDTDVGKTVASKAILHALAAKDLRTIGYKPVAAGSDKTPEGWRNSDALHLQKAATVAVDYEDINPYALELP
ASPHIAAKHEKVEIDYAVLSRKLAQHKENADVVLVEGAGGWRVPVSDTDSLSTWVKQEDLPVVLVVGIKLGCLSHALLTA
EVIKADGLNLVGWVANRVNPGTEHYAEIIDMLEERLEVPKLGEIPYIPSAKRKDLGKYINADVLLEL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI87081944, Length=224, Percent_Identity=53.125, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=52.4444444444444, Blast_Score=237, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_VIBVU (Q8D8N2)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761744.1
- ProteinModelPortal:   Q8D8N2
- SMR:   Q8D8N2
- GeneID:   1179774
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2940
- HOGENOM:   HBG650065
- OMA:   ALAAYWQ
- ProtClustDB:   PRK00090
- BioCyc:   VVUL216895:VV1_2940-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24637; Mature: 24637

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAFFIAGTDTDVGKTVASKAILHALAAKDLRTIGYKPVAAGSDKTPEGWRNSDALHLQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
KAATVAVDYEDINPYALELPASPHIAAKHEKVEIDYAVLSRKLAQHKENADVVLVEGAGG
EEEEEEEEECCCCCEEEECCCCCCEECCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCC
WRVPVSDTDSLSTWVKQEDLPVVLVVGIKLGCLSHALLTAEVIKADGLNLVGWVANRVNP
CCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
GTEHYAEIIDMLEERLEVPKLGEIPYIPSAKRKDLGKYINADVLLEL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MMKAFFIAGTDTDVGKTVASKAILHALAAKDLRTIGYKPVAAGSDKTPEGWRNSDALHLQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
KAATVAVDYEDINPYALELPASPHIAAKHEKVEIDYAVLSRKLAQHKENADVVLVEGAGG
EEEEEEEEECCCCCEEEECCCCCCEECCCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCC
WRVPVSDTDSLSTWVKQEDLPVVLVVGIKLGCLSHALLTAEVIKADGLNLVGWVANRVNP
CCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
GTEHYAEIIDMLEERLEVPKLGEIPYIPSAKRKDLGKYINADVLLEL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA