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The map label for this gene is topB [H]

Identifier: 37679339

GI number: 37679339

Start: 1168119

End: 1170089

Strand: Direct

Name: topB [H]

Synonym: VV1155

Alternate gene names: 37679339

Gene position: 1168119-1170089 (Clockwise)

Preceding gene: 37679338

Following gene: 37679340

Centisome position: 34.82

GC content: 50.94

Gene sequence:

>1971_bases
ATGACCCGTCTGTTTATCGCTGAAAAACCCAGCTTAGGCCGCGCGATTGCTGCCGCATTGCCCAATCCTCAAAAGAAAGA
ACAAGGCTTTATTCGCTGTGGCAATGGTGACATCGTGACGTGGTGTATTGGACATTTGCTGGAGCAAGTTGAGCCCGATG
CTTACGACGAACGTTATAAAAAATGGAATATGGCGGATTTGCCCATCATCCCTGAGCAATGGCAACTGCGCCCACGTCCA
TCGGCCAGTAAACAATTGACCGTGATTCGCAAGCTGCTAAAAGAAGCAAGTGAAGTGATCCATGCAGGCGACCCCGACAG
AGAAGGGCAATTGCTGGTGGATGAAGTGCTGGATTACTGCAAACTGAGCCAAACCAAAAAAGCACAAACCCAGCGATTGT
TGATCAGCGATCTCAACCTGCCTGCGGTTAAGCGCGCGTTGAGTAGTCTACGCAGCAATCGTGACTTTGTTCCTCTGTCG
GTTTCCGCACTGGCACGTTCTCGGGCTGATTGGCTGTATGGTATGAACATGTCACGTGCTTACACTTTGTTGGGGCAAAA
AGCAGGCTATCAAGGTGTGCTGTCTGTCGGACGAGTACAAACGCCGGTATTGGGGCTGGTGGTGCGCCGTGATGAGGAAA
TCGAGAATTTCGTTCCGCGTGATTTCTTCACTCTTCACGCGCTGATCCCTTATCAGAATGGCAGTGAACCGTTTGACATC
AAAGCACGTTGGAAACCGAGCGAAGCTTGCCAACCTTGGCAAGACGAAGAAGGGCGAGTCTTGAGTCGTAAACTGGTGGA
AAACGTTGCCAGTCGTATTGCCAATCAACCCGCTACCGTGACGGAATCGGAACACAAACAGACCAAGCAAGCCCCGCCTT
TGCCTTATTCGCTGTCGGCGCTGCAAATTGATGCGGCTAAACGCTATGGTATGAGTGCACAACAGGTGCTGGATACGTGT
CAGTCACTGTACGAAAAGCACAAACTGATCACCTACCCACGCTCGGATAACCGGTATCTTCCTAAAGAACATTATGCGCA
AGCCGCCAGTGTCAGCCAAGCCATTGCCAATAATGCGAAAGAGCTGTCCGATGCGGTGAGTGGCGCTGATCTTTCTTTAA
AATCTAAAGCGTGGAACGACAGCAAAGTCGAAGCCCACCACGCGATTATCCCCACGCCGAAACAAGCCAGTGTTAACGCG
CTGTCTGGTTACGAAATGAAGATCTATCAATTGATCGCCAGACAGTATTTGATGCAGTTCTATCCCGCTGCCACATACGC
TGAAGCCAAACTGGTGTTTGAGATTGCTGGTGGTGTCTTTATTGCGAAAGGTCGCCAATTGACATTTGCCGGTTGGAAAG
CACTCACGGGAGCAACAGAAAAAGAAGAGGACGGCGCGGATACTGTACCACCTCTTGCGAAAGGCACGGTGCTCACCTGC
CGAGAAGGGGTAATTAAAGACTGTAAAACCGAGCCGCCAAAGCACTTTACCGAAGCGACCTTATTGCAGGCGATGACGGG
AATTGCACGTTACGTCAGTGATAAAGAACTGAAAAAAATCTTAAGAGAGACCGACGGATTAGGCACGGAAGCGACCAGAG
CGGGCATACTCGATACTCTGTTTAAACGTCAGTTACTCTCTCGACAAGGCAAAGCCATTCTTAGTACTCCAGCAGGGAGA
GGGTTGATCCATGCGTTGCCACAAGAATCAACTTATCCGGATATGACGGCTCACTGGGAGCACCAATTGCAAGGCATGGC
TGAACGCAACCAAGCCTACCAGCCATTTATGGCCGCCCTACAACAGCGTATTTCGGAATTAATGCAGCAGGTCACAACAT
CACCCGTGCCGAGCTCGCTGCGTCAACTGCCACCAGTGGAGCGCCCAGCGTTTAAGCGCAAGAGACGCGCCAGTACCAGC
AAGAAAACGGCGTCAACCGCGACCCAAAAAAGAACCAACAAGTCGGCTTAA

Upstream 100 bases:

>100_bases
GTTGAGTATCTATAACTGTCTATACATACAGTTTTTGTTGATTTTTAAGGGCCACATCCGCTAATGTGGCCCTTTGTTTG
TTGAGCTCGAAAAATTTGTT

Downstream 100 bases:

>100_bases
ACCCCATGATGAGCATCCCTCTGATATATCACCCAATCTACTCTCGGTTGCCCCTACCTGAGGGGCATCGTTACCCGATC
ATGAAATATCACCATCTCTA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRP
SASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALSSLRSNRDFVPLS
VSALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTC
QSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELSDAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNA
LSGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGR
GLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVTTSPVPSSLRQLPPVERPAFKRKRRASTS
KKTASTATQKRTNKSA

Sequences:

>Translated_656_residues
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRP
SASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALSSLRSNRDFVPLS
VSALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTC
QSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELSDAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNA
LSGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGR
GLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVTTSPVPSSLRQLPPVERPAFKRKRRASTS
KKTASTATQKRTNKSA
>Mature_655_residues
TRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYKKWNMADLPIIPEQWQLRPRPS
ASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYCKLSQTKKAQTQRLLISDLNLPAVKRALSSLRSNRDFVPLSV
SALARSRADWLYGMNMSRAYTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDIK
ARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSALQIDAAKRYGMSAQQVLDTCQ
SLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAKELSDAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNAL
SGYEMKIYQLIARQYLMQFYPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTCR
EGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTLFKRQLLSRQGKAILSTPAGRG
LIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAALQQRISELMQQVTTSPVPSSLRQLPPVERPAFKRKRRASTSK
KTASTATQKRTNKSA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=22.3472668810289, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788061, Length=615, Percent_Identity=60.3252032520325, Blast_Score=765, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=545, Percent_Identity=26.605504587156, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=23.3226837060703, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=632, Percent_Identity=23.4177215189873, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323263, Length=396, Percent_Identity=25.5050505050505, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585251, Length=686, Percent_Identity=23.7609329446064, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73159; Mature: 73027

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYK
CCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRPSASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYC
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSQTKKAQTQRLLISDLNLPAVKRALSSLRSNRDFVPLSVSALARSRADWLYGMNMSRA
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCCEEEEEEEECCCCCCCCEE
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHH
LQIDAAKRYGMSAQQVLDTCQSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAK
HEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELSDAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNALSGYEMKIYQLIARQYLMQF
HHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
YPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
CCCCHHHHHHEEEEECCCEEEECCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEHH
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRQLLSRQGKAILSTPAGRGLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAAL
HHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRISELMQQVTTSPVPSSLRQLPPVERPAFKRKRRASTSKKTASTATQKRTNKSA
HHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRLFIAEKPSLGRAIAAALPNPQKKEQGFIRCGNGDIVTWCIGHLLEQVEPDAYDERYK
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHH
KWNMADLPIIPEQWQLRPRPSASKQLTVIRKLLKEASEVIHAGDPDREGQLLVDEVLDYC
CCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSQTKKAQTQRLLISDLNLPAVKRALSSLRSNRDFVPLSVSALARSRADWLYGMNMSRA
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCHHHH
YTLLGQKAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVPRDFFTLHALIPYQNGSEPFDI
HHHHHHCCCCHHHHHHCCHHCCHHHHHHHCCHHHHHCCCCCCEEEEEEEECCCCCCCCEE
KARWKPSEACQPWQDEEGRVLSRKLVENVASRIANQPATVTESEHKQTKQAPPLPYSLSA
EECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHH
LQIDAAKRYGMSAQQVLDTCQSLYEKHKLITYPRSDNRYLPKEHYAQAASVSQAIANNAK
HEEHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELSDAVSGADLSLKSKAWNDSKVEAHHAIIPTPKQASVNALSGYEMKIYQLIARQYLMQF
HHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHH
YPAATYAEAKLVFEIAGGVFIAKGRQLTFAGWKALTGATEKEEDGADTVPPLAKGTVLTC
CCCCHHHHHHEEEEECCCEEEECCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCEEHH
REGVIKDCKTEPPKHFTEATLLQAMTGIARYVSDKELKKILRETDGLGTEATRAGILDTL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FKRQLLSRQGKAILSTPAGRGLIHALPQESTYPDMTAHWEHQLQGMAERNQAYQPFMAAL
HHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQRISELMQQVTTSPVPSSLRQLPPVERPAFKRKRRASTSKKTASTATQKRTNKSA
HHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]