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The map label for this gene is menF [H]

Identifier: 37679299

GI number: 37679299

Start: 1122568

End: 1123890

Strand: Direct

Name: menF [H]

Synonym: VV1115

Alternate gene names: 37679299

Gene position: 1122568-1123890 (Clockwise)

Preceding gene: 37679295

Following gene: 37679300

Centisome position: 33.46

GC content: 47.69

Gene sequence:

>1323_bases
ATGTACGAGGTTGTTTTGTCACATTTTCATCAAGCCATTACCAATCTCATTGAACGTGTCAAAAATGCCAAGGATGACGA
GACTCGTCTGGTCGAGCTTTTAACAACAAAACCTACATTGACGTTCATTGACTGGCTTGAAGCCCAGCCGGTTTTTCCAA
AATTTTATTGGCAGTCACGAGATACTCGTGAAGAGGTGGTCGCGTTAGGACAATTGCACACCTTTGTCGATCCAGGCCCG
GCCTATACCATACTGTCTCCCGGACAACGTGTTTGGGGAGGACGATCATTCGATGGCAGCAGCGAGAAAAATCGCCGTTG
TATGCCTTCTTTTTTCTTTTTACCTCAGATTGAACTGATCCGTTTGGACGACAAGTGGTCGTTGGCGGTGAATTTAAATG
GTGATAAGAGCCGAATTCTGCACACGTTGAGCAAACTCACGACTGAGGCGAAACCGTTAGCACCTCTCACCGCACACATC
CAGAGCGTGGAACACTTACCTGAGCGAGAGGGGTGGTGTGACTTGGTGAGTAAAGTGCTGAAAGGCATACAAGCTAAGGC
GTTTAAGAAGGTCGTTCTTGCACGTAAAACCACAGTGCATTTAGATTCCGCATTGTGCGCGGCGCAGTTGCTTAAGGCAA
GTTATCTGCAAAATCACCACAGTTTTCACTTTATGCTGAGTCTCGACAGTAAACACAGTTTCATCGGCTCAACACCAGAG
AGACTTTACTTGCGTCAAGGGCAAGAGTTGTATACCGAAGCATTGGCAGGCACCATTGGTCGTGGCCACAACGCTACGCA
AGATATGGAACTGGCCAGTTGGCTTTCACAGGATGTCAAAAATCTGAATGAAAACCAGTATGTGGTTGACGATATCGTTG
ATCGCCTTACACCTCATGCTGCGAGTGTAGAGGTTGAGTCTGAAGCGCGTTTAGTGCGCCTGCGTAAAGTTCAGCATCTG
AAGCGCAATATTCATGCTCAGTTGAAGGCGGGAGTCAATGGAGTACAACTGCTCTCGGCTTTACAACCAACCGCGGCGGT
TGCAGGATTGCCTCGTCAAGAGTCCATGCAGTTTATTGTTGAAAATGAACCTTTCGCTCGTGGCTGGTATGCTGGTTCTG
TTGGCTACATTAGCCATGAACAAGCGGAGTTTTGTGTGGCAATACGCAGCGCTTTGATTGTGGATGATTCACTGCATTTG
TTCGCTGGAGCTGGCATTGTTCCTGGCTCAATCGCAGAGCATGAATGGAGTGAACTGGATAAAAAAATGTCGACCTTATT
GTCACTGATTTCTGAGCACCCACCGCTTGGAGTGGCATCATGA

Upstream 100 bases:

>100_bases
GGAGGCGTCAGTTATCACAGGATTCTGCAAAAGATCGCACACAACCTTGATTTGATCAGGGTCTATCAATAGAGTAGCGA
CAGTAATTCTTTATCCTCTA

Downstream 100 bases:

>100_bases
ACCACGATCAAGCCGTATTGAATCGTATTTGGTGTGAAACGCTGTTTGAAGAATTGTACCGCTTTGGGGTACGAGATGTT
TGTGTCGCACCTGGCTCACG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MYEVVLSHFHQAITNLIERVKNAKDDETRLVELLTTKPTLTFIDWLEAQPVFPKFYWQSRDTREEVVALGQLHTFVDPGP
AYTILSPGQRVWGGRSFDGSSEKNRRCMPSFFFLPQIELIRLDDKWSLAVNLNGDKSRILHTLSKLTTEAKPLAPLTAHI
QSVEHLPEREGWCDLVSKVLKGIQAKAFKKVVLARKTTVHLDSALCAAQLLKASYLQNHHSFHFMLSLDSKHSFIGSTPE
RLYLRQGQELYTEALAGTIGRGHNATQDMELASWLSQDVKNLNENQYVVDDIVDRLTPHAASVEVESEARLVRLRKVQHL
KRNIHAQLKAGVNGVQLLSALQPTAAVAGLPRQESMQFIVENEPFARGWYAGSVGYISHEQAEFCVAIRSALIVDDSLHL
FAGAGIVPGSIAEHEWSELDKKMSTLLSLISEHPPLGVAS

Sequences:

>Translated_440_residues
MYEVVLSHFHQAITNLIERVKNAKDDETRLVELLTTKPTLTFIDWLEAQPVFPKFYWQSRDTREEVVALGQLHTFVDPGP
AYTILSPGQRVWGGRSFDGSSEKNRRCMPSFFFLPQIELIRLDDKWSLAVNLNGDKSRILHTLSKLTTEAKPLAPLTAHI
QSVEHLPEREGWCDLVSKVLKGIQAKAFKKVVLARKTTVHLDSALCAAQLLKASYLQNHHSFHFMLSLDSKHSFIGSTPE
RLYLRQGQELYTEALAGTIGRGHNATQDMELASWLSQDVKNLNENQYVVDDIVDRLTPHAASVEVESEARLVRLRKVQHL
KRNIHAQLKAGVNGVQLLSALQPTAAVAGLPRQESMQFIVENEPFARGWYAGSVGYISHEQAEFCVAIRSALIVDDSLHL
FAGAGIVPGSIAEHEWSELDKKMSTLLSLISEHPPLGVAS
>Mature_440_residues
MYEVVLSHFHQAITNLIERVKNAKDDETRLVELLTTKPTLTFIDWLEAQPVFPKFYWQSRDTREEVVALGQLHTFVDPGP
AYTILSPGQRVWGGRSFDGSSEKNRRCMPSFFFLPQIELIRLDDKWSLAVNLNGDKSRILHTLSKLTTEAKPLAPLTAHI
QSVEHLPEREGWCDLVSKVLKGIQAKAFKKVVLARKTTVHLDSALCAAQLLKASYLQNHHSFHFMLSLDSKHSFIGSTPE
RLYLRQGQELYTEALAGTIGRGHNATQDMELASWLSQDVKNLNENQYVVDDIVDRLTPHAASVEVESEARLVRLRKVQHL
KRNIHAQLKAGVNGVQLLSALQPTAAVAGLPRQESMQFIVENEPFARGWYAGSVGYISHEQAEFCVAIRSALIVDDSLHL
FAGAGIVPGSIAEHEWSELDKKMSTLLSLISEHPPLGVAS

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=408, Percent_Identity=39.2156862745098, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1786809, Length=271, Percent_Identity=29.8892988929889, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787518, Length=307, Percent_Identity=28.6644951140065, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788114, Length=218, Percent_Identity=29.3577981651376, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=370, Percent_Identity=24.8648648648649, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49248; Mature: 49248

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEVVLSHFHQAITNLIERVKNAKDDETRLVELLTTKPTLTFIDWLEAQPVFPKFYWQSR
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCC
DTREEVVALGQLHTFVDPGPAYTILSPGQRVWGGRSFDGSSEKNRRCMPSFFFLPQIELI
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCEEECCCEEEE
RLDDKWSLAVNLNGDKSRILHTLSKLTTEAKPLAPLTAHIQSVEHLPEREGWCDLVSKVL
EECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGIQAKAFKKVVLARKTTVHLDSALCAAQLLKASYLQNHHSFHFMLSLDSKHSFIGSTPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
RLYLRQGQELYTEALAGTIGRGHNATQDMELASWLSQDVKNLNENQYVVDDIVDRLTPHA
HHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCC
ASVEVESEARLVRLRKVQHLKRNIHAQLKAGVNGVQLLSALQPTAAVAGLPRQESMQFIV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHCCCCHHCCCEEE
ENEPFARGWYAGSVGYISHEQAEFCVAIRSALIVDDSLHLFAGAGIVPGSIAEHEWSELD
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHH
KKMSTLLSLISEHPPLGVAS
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYEVVLSHFHQAITNLIERVKNAKDDETRLVELLTTKPTLTFIDWLEAQPVFPKFYWQSR
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCC
DTREEVVALGQLHTFVDPGPAYTILSPGQRVWGGRSFDGSSEKNRRCMPSFFFLPQIELI
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCEEECCCEEEE
RLDDKWSLAVNLNGDKSRILHTLSKLTTEAKPLAPLTAHIQSVEHLPEREGWCDLVSKVL
EECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGIQAKAFKKVVLARKTTVHLDSALCAAQLLKASYLQNHHSFHFMLSLDSKHSFIGSTPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
RLYLRQGQELYTEALAGTIGRGHNATQDMELASWLSQDVKNLNENQYVVDDIVDRLTPHA
HHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCC
ASVEVESEARLVRLRKVQHLKRNIHAQLKAGVNGVQLLSALQPTAAVAGLPRQESMQFIV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHCCCCHHCCCEEE
ENEPFARGWYAGSVGYISHEQAEFCVAIRSALIVDDSLHLFAGAGIVPGSIAEHEWSELD
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHH
KKMSTLLSLISEHPPLGVAS
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]