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The map label for this gene is seqA [H]

Identifier: 37679203

GI number: 37679203

Start: 1017007

End: 1017540

Strand: Direct

Name: seqA [H]

Synonym: VV1019

Alternate gene names: 37679203

Gene position: 1017007-1017540 (Clockwise)

Preceding gene: 37679199

Following gene: 37679204

Centisome position: 30.32

GC content: 42.7

Gene sequence:

>534_bases
ATGAAAACAATTGAGGTTGATGAGGATCTATACCGTTACATTGCGAGTCAAACCCAACATATTGGCGAAAGTGCATCGGA
TATTTTGCGCCGCTTATTGAATGTGGATGGACGTCAGACTGTTGCCCCTATTACGATAGAACCGAAAGGTATCGTAGTAA
GCAAAGATGCAGGCAAAGACACTCAACTTGATGGCGTAAAAGAGATGCGCTCACTATTAATTTCGGATGAGTTTGCCTCA
TTAAAGAAAGCCATCGATCGATTTATGCTTGTGCTGAGCACGCTACATCGAATCAACCCTGAGTCGTTTGCTCAAGCGAC
AAAAGTGAAAGGTCGTAAGCGTGTTTATTTCGCCGACAATGAGGCGACATTGCTAGAAAATGGCAATACGACGAAACCAA
AATCCATTCCAGGCACGGGTTTTTGGGTCATCACTAATAACAATACCAGCCGTAAGAGGCAAATGGTGGAGCAAGTGATG
GTGCATATGGAATTCCAGCCTGATTTAATTGAAAAAGTAACAGGTTCAATTTAA

Upstream 100 bases:

>100_bases
GCTGACATTTTATCTTGTTCGTGATGGGTTTAACGTGGAGTATCCGCGAGGATCTCGTTATAATCCCCCAGAGTTTAAAC
ATTGAGATTGTGAAAAGCGA

Downstream 100 bases:

>100_bases
GAAACCTGATAGAAGCCTCATAATGCGCAAGATTATGCCGATATTATGAGGCTTTTTTGTTTGAGTATATTTTAAGAAAG
GATGTCATTAATGGCTATGC

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MKTIEVDEDLYRYIASQTQHIGESASDILRRLLNVDGRQTVAPITIEPKGIVVSKDAGKDTQLDGVKEMRSLLISDEFAS
LKKAIDRFMLVLSTLHRINPESFAQATKVKGRKRVYFADNEATLLENGNTTKPKSIPGTGFWVITNNNTSRKRQMVEQVM
VHMEFQPDLIEKVTGSI

Sequences:

>Translated_177_residues
MKTIEVDEDLYRYIASQTQHIGESASDILRRLLNVDGRQTVAPITIEPKGIVVSKDAGKDTQLDGVKEMRSLLISDEFAS
LKKAIDRFMLVLSTLHRINPESFAQATKVKGRKRVYFADNEATLLENGNTTKPKSIPGTGFWVITNNNTSRKRQMVEQVM
VHMEFQPDLIEKVTGSI
>Mature_177_residues
MKTIEVDEDLYRYIASQTQHIGESASDILRRLLNVDGRQTVAPITIEPKGIVVSKDAGKDTQLDGVKEMRSLLISDEFAS
LKKAIDRFMLVLSTLHRINPESFAQATKVKGRKRVYFADNEATLLENGNTTKPKSIPGTGFWVITNNNTSRKRQMVEQVM
VHMEFQPDLIEKVTGSI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=183, Percent_Identity=55.7377049180328, Blast_Score=199, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 19922; Mature: 19922

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDEDLYRYIASQTQHIGESASDILRRLLNVDGRQTVAPITIEPKGIVVSKDAGKD
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCC
TQLDGVKEMRSLLISDEFASLKKAIDRFMLVLSTLHRINPESFAQATKVKGRKRVYFADN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECC
EATLLENGNTTKPKSIPGTGFWVITNNNTSRKRQMVEQVMVHMEFQPDLIEKVTGSI
CEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKTIEVDEDLYRYIASQTQHIGESASDILRRLLNVDGRQTVAPITIEPKGIVVSKDAGKD
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCC
TQLDGVKEMRSLLISDEFASLKKAIDRFMLVLSTLHRINPESFAQATKVKGRKRVYFADN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECC
EATLLENGNTTKPKSIPGTGFWVITNNNTSRKRQMVEQVMVHMEFQPDLIEKVTGSI
CEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]