Click here to switch to the map view.

The map label for this gene is nagE [H]

Identifier: 37679196

GI number: 37679196

Start: 1010486

End: 1012057

Strand: Direct

Name: nagE [H]

Synonym: VV1012

Alternate gene names: 37679196

Gene position: 1010486-1012057 (Clockwise)

Preceding gene: 37679190

Following gene: 37679197

Centisome position: 30.12

GC content: 47.33

Gene sequence:

>1572_bases
GTGAATATTCTTGGATACGCGCAAAAGTTAGGTAAAGCTCTTATGCTACCTATCGCAACGCTACCAGTTGCGGCGCTCTT
ACTACGTTTGGGTCAAGGCGACCTGTTAGATATCCCATTCATGGCGCAAGCTGGTGGTGCTATCTTTGGTCAACTTCCTC
TTCTATTCGGTCTTGGTATCGCGATTGGTCTTTCTAAAGACGGTCAAGGTGCTGCAGGTCTAGCAGGTGCAGTGGCGTAC
TTTGTACTAACTGCAACAGCAACAACAATCGATGCTTCTATCAACATGTCGTTCTTTGGCGGTATCATTGGTGGTATCAT
CGCTGGTCACTCATACAACGCTTTCCATGCTACACGTCTTCCTGAGTGGTTGGCATTCTTCTCTGGCAAACGTCTAGTGC
CTATTATGGCAGGTCTATTCTCACTGGTTGCTGGTGCAGTTGCTGGTGTGGTTTGGCCTTCAATCCAAGGCGGTCTTGAT
GCTCTAGCACACGCGGTATCGACTTCTGGTGCTGTAGGTCAATTCGTTTACGGTACTCTAAACCGTGCACTAATTCCTGT
TGGTCTTCACCACGTTCTTAACTCATACTTCTGGTTCGGTATGGGTACGTGTCAAGAGATCCTAGTAACAGGTGCTCAAG
CAGCTGGTCAAGCTCTACCTGCTCTACAACAGCTTTGTGTAGACCCAGCACTAGCGAAAACTCTTGTTGCTGGTCAAACT
CACACATTTGAGTTTGCTAACGCTGTAACTCCTGAAATCACTGCAACAGTGAAAGAAGTAACTGAAACAGTGAAATCTGG
TGACCTACACCGCTTCTTCGGTGGTGACAAAGGCGCTGGCGTATTCATGAACGGCTTCTTCCCTGTGATGATGTTTGGTC
TACCAGGTGCTGCTCTAGCGATGTACCTAGCAGCTCCAGCAGAAAAACGTAGCCAAGTTGGTGGTGCACTGTTCTCTGTA
GCGTTCTGTTCATTCCTAACCGGTATCACAGAGCCACTAGAATTCATGTTCGTGTTCCTAGCTCCAGCACTATACGCTCT
ACACGCTGTGTTCACTGGTCTAGCACTAGTTGTAGCAAACATGTTCGGCACGCTACATGGCTTTGGTTTCTCAGCAGGTC
TAATCGACTTCCTATTGAACTGGGGTCTAGCAACTAAGCCATTCGTACTGCTACTAATCGGTCTAGCTTTCGGTGCTCTA
TACTTCTTCACTTTCTTCTTCGCAATCCGCGCATTCAACCTGAAATCGCCAGGTCGTGAAGATGATGATTCTGAAACAAG
TGCGGCTTCTGGTGATGCAAAGAGTGGTGACCTAGCTCGTCAATACCTAAAAGCACTAGGTGGTCACGAGAACCTTACAT
CAATCGATGCATGTATCACTCGTCTACGTCTAACGCTTAAAGATCGCTCTGTCGCTGACGAAGCAGTACTTAAGAAACTG
GGTGCTAAAGGTGTGGTTAAGCTTGGCGAAAACAACCTACAAGTTATCCTTGGTCCTCTAGCTGAAATCGTTGCGGGTGA
AATGAAAGCAATCGGTGTCAACGAAGACCTATCAGAAGTGAAACTGCCTTAA

Upstream 100 bases:

>100_bases
ATTTGCGGAGCAAAATTAATGTCATAAACTGGAAAAGACTAAATTGGACGACTAAAAAAAGTCTTCCAAAAAAATGAAAA
TCCTATAGGGGGAACTTAAG

Downstream 100 bases:

>100_bases
TAGCAATCACTCTGAATGATATGAAAAGCCTCCTTCGGGAGGCTTTTTCTGTTTTGGCACTCTATCTTGGTCTTTTCAGA
CAAATAATCCCATTTAAACG

Product: PTS system, N-acetylglucosamine-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQGDLLDIPFMAQAGGAIFGQLPLLFGLGIAIGLSKDGQGAAGLAGAVAY
FVLTATATTIDASINMSFFGGIIGGIIAGHSYNAFHATRLPEWLAFFSGKRLVPIMAGLFSLVAGAVAGVVWPSIQGGLD
ALAHAVSTSGAVGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVTGAQAAGQALPALQQLCVDPALAKTLVAGQT
HTFEFANAVTPEITATVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYALHAVFTGLALVVANMFGTLHGFGFSAGLIDFLLNWGLATKPFVLLLIGLAFGAL
YFFTFFFAIRAFNLKSPGREDDDSETSAASGDAKSGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAIGVNEDLSEVKLP

Sequences:

>Translated_523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQGDLLDIPFMAQAGGAIFGQLPLLFGLGIAIGLSKDGQGAAGLAGAVAY
FVLTATATTIDASINMSFFGGIIGGIIAGHSYNAFHATRLPEWLAFFSGKRLVPIMAGLFSLVAGAVAGVVWPSIQGGLD
ALAHAVSTSGAVGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVTGAQAAGQALPALQQLCVDPALAKTLVAGQT
HTFEFANAVTPEITATVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYALHAVFTGLALVVANMFGTLHGFGFSAGLIDFLLNWGLATKPFVLLLIGLAFGAL
YFFTFFFAIRAFNLKSPGREDDDSETSAASGDAKSGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAIGVNEDLSEVKLP
>Mature_523_residues
MNILGYAQKLGKALMLPIATLPVAALLLRLGQGDLLDIPFMAQAGGAIFGQLPLLFGLGIAIGLSKDGQGAAGLAGAVAY
FVLTATATTIDASINMSFFGGIIGGIIAGHSYNAFHATRLPEWLAFFSGKRLVPIMAGLFSLVAGAVAGVVWPSIQGGLD
ALAHAVSTSGAVGQFVYGTLNRALIPVGLHHVLNSYFWFGMGTCQEILVTGAQAAGQALPALQQLCVDPALAKTLVAGQT
HTFEFANAVTPEITATVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALAMYLAAPAEKRSQVGGALFSV
AFCSFLTGITEPLEFMFVFLAPALYALHAVFTGLALVVANMFGTLHGFGFSAGLIDFLLNWGLATKPFVLLLIGLAFGAL
YFFTFFFAIRAFNLKSPGREDDDSETSAASGDAKSGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
GAKGVVKLGENNLQVILGPLAEIVAGEMKAIGVNEDLSEVKLP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=518, Percent_Identity=48.4555984555985, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787343, Length=527, Percent_Identity=35.673624288425, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1787908, Length=552, Percent_Identity=33.5144927536232, Blast_Score=262, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 54648; Mature: 54648

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGYAQKLGKALMLPIATLPVAALLLRLGQGDLLDIPFMAQAGGAIFGQLPLLFGLGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCCHHHHHHHHHHHHHH
AIGLSKDGQGAAGLAGAVAYFVLTATATTIDASINMSFFGGIIGGIIAGHSYNAFHATRL
HEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
PEWLAFFSGKRLVPIMAGLFSLVAGAVAGVVWPSIQGGLDALAHAVSTSGAVGQFVYGTL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
NRALIPVGLHHVLNSYFWFGMGTCQEILVTGAQAAGQALPALQQLCVDPALAKTLVAGQT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
HTFEFANAVTPEITATVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALA
CCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHH
MYLAAPAEKRSQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYALHAVFTGLALVVAN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFGTLHGFGFSAGLIDFLLNWGLATKPFVLLLIGLAFGALYFFTFFFAIRAFNLKSPGRE
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DDDSETSAASGDAKSGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKGVVKLGENNLQVILGPLAEIVAGEMKAIGVNEDLSEVKLP
CCCCEEEECCCCCEEHHHHHHHHHHCCHHEECCCCCHHHCCCC
>Mature Secondary Structure
MNILGYAQKLGKALMLPIATLPVAALLLRLGQGDLLDIPFMAQAGGAIFGQLPLLFGLGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHCCCHHHHHHHHHHHHHH
AIGLSKDGQGAAGLAGAVAYFVLTATATTIDASINMSFFGGIIGGIIAGHSYNAFHATRL
HEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
PEWLAFFSGKRLVPIMAGLFSLVAGAVAGVVWPSIQGGLDALAHAVSTSGAVGQFVYGTL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
NRALIPVGLHHVLNSYFWFGMGTCQEILVTGAQAAGQALPALQQLCVDPALAKTLVAGQT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
HTFEFANAVTPEITATVKEVTETVKSGDLHRFFGGDKGAGVFMNGFFPVMMFGLPGAALA
CCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEHHHHHHHHHCCCHHHHH
MYLAAPAEKRSQVGGALFSVAFCSFLTGITEPLEFMFVFLAPALYALHAVFTGLALVVAN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFGTLHGFGFSAGLIDFLLNWGLATKPFVLLLIGLAFGALYFFTFFFAIRAFNLKSPGRE
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DDDSETSAASGDAKSGDLARQYLKALGGHENLTSIDACITRLRLTLKDRSVADEAVLKKL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKGVVKLGENNLQVILGPLAEIVAGEMKAIGVNEDLSEVKLP
CCCCEEEECCCCCEEHHHHHHHHHHCCHHEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]