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The map label for this gene is ligA

Identifier: 37679166

GI number: 37679166

Start: 980812

End: 982821

Strand: Direct

Name: ligA

Synonym: VV0982

Alternate gene names: 37679166

Gene position: 980812-982821 (Clockwise)

Preceding gene: 37679165

Following gene: 37679167

Centisome position: 29.24

GC content: 48.56

Gene sequence:

>2010_bases
ATGTCTGAATCCAAGCAGCAATATTTAGAAGAATTAAAACAGCAACTGCACTATCACGCAGTTCGTTACTACGTTGAAGA
TAATCCTGAAATACCGGATGCCGAATATGACCGTATGATGCGTGAGTTAATGGCCATTGAAGCGGAGCATCCGGAGTGGA
TTAGTGTTGACTCACCGAGTCAGCGAGTGGGAGGTGTCGCTCTTGATAGTTTCCGCCAAGTGACGCATGAGATCCCGATG
CTGTCTTTGGACAACGCTTTTAGCGATGAAGAGCTCGAGAGTTTCCTTAAACGCGCACAAGATCGTATGCCAAGTGCTCA
TATTGATGCATTTTGTTGCGAGCCTAAGCTTGATGGTTTAGCTGTCAGCCTTCTCTATGAAAATGGAGTTTTGGTTCAGG
CCGCGACTCGAGGCGATGGCACCACAGGCGAAAATATTACCGAAAACGTGCGTACCATTGCTTCTGTGCCACTGAAATTG
CAGGGAGAAGGTTGGCCATCACGCATCGAAGTGCGTGGTGAAGTTTTCATGCCAAAAGTGGGCTTTGAAAAACTCAACGA
CATCGCTCGCAAGAAAGGCGAAAAAGTATTCGTTAATCCGCGCAATGCAGCGGCGGGAAGCTTACGCCAGCTTGATTCGA
AGATCACCGCAACTCGTCCGTTGGCCTTTTATGCGTATAGCGTGGGCGTCGTAGAGGGCATCTCACTGTCATCAAGCCAT
TACCAGCGTTTCTTGCAGTTAAAGCAATGGGGCTTGCCTATGTGCCCTGAGACGAAGTTGGTTAATGGGTTGGAAAGCGT
TAAGGCATTCTACGCAGACATCTTAAATCGACGCGATGCATTGCCATATGAAATCGATGGTGTCGTGATTAAGATTGACG
ACATTGTGATTCAAGAGAAATTAGGTTTTGTGGCACGCGCACCGCGTTGGGCTATCGCGTATAAGTTTCCTGCGCAAGAA
GAACTCACCCTGTTGAATGACGTTGAGTTTCAAGTTGGCAGAACGGGGGCTATTACTCCAGTCGCGAAGCTGGAACCTGT
TTTCGTCGGTGGTGTTACCGTCAGTAATGCAACGCTTCATAACGCTGATGAAATCGAACGCCTTGGTGTGATGGTTGGTG
ATACCGTCGTGATTCGACGCGCTGGGGATGTGATTCCACAAATTGTTTCTGTGGTGAAAGAGCGCCGTAAAGGGGATGAA
AACCCCATCGTTTTCCCGACCTCTTGCCCAGTCTGTCACTCACATGTTGAACGGATTGAGGGAGAAGCGGTAACTCGCTG
CTCTGGCGGTTTGGTGTGTCAAGCGCAGCGAAAAGAGGCGCTGAAACATTTTGTGTCTAGGAAGGCGCTCGATGTCGATG
GGTTGGGTGACAAGGTCATAGAGCAGCTTGTAGATAAAGAAATGGTAGAGACTCCAGCGGACTTGTTTACGTTATCTGCA
GGCGTTTTAACGGTACTTGAACGAATGGGACCAAAGTCAGCCCAGAACATCGTCAACGCACTGAACGTTGCGAAAGAGAC
TACGTTGGCTCGTTTTCTTTATTCTCTGGGGATCCGAGAAGTGGGCGAGGCAACCGCGGCTAATTTGGCTCGTCATTTTA
AGACACTAGAAGCGATACAAACTGCAACGCATGAGCAGTTAATCGAAGTTCCCGATATCGGTGAAGTCGTCGCTCGTCAT
ATCACGGCATTTTTTGCTGAAGAGAAAAATCAAAGAGTGGTGCAAGCACTTATTGAACAGGGAATTGTGTGGCCCCCTGT
CGAGGAGCTTGGTAGTGAGATACCTCAGCCATTAGCAGGCAAAGTTGTAGTACTAACCGGGACCTTGACGCAACTCAGCC
GTGGCGATGCAAAAGCGGCTTTGGAACGATTGGGGGCTAAAGTCACGGGCAGTGTATCCAAGAAAACAGATATCGTTTTT
GCTGGTGAAGCGGCGGGTTCAAAATTAACCAAAGCGCAAGAACTTGGCGTGGAAATTCAAACAGAGCAAGATTTACTCGC
ACTTCTGTAA

Upstream 100 bases:

>100_bases
AGGCAAATAGTTGAGTTCAAAGCGGCAAACGCTTAAGCTTCCAATATCCCAACAGGGCTCCCTAAGGAGCCCTTTTTTAT
CGTGATTTAAGGGCTGGAAA

Downstream 100 bases:

>100_bases
CCTAAAAAAGATAAAATAAAAACTAAAACCCTCTGAATTGACGTTTGGAGGGTTATTTTTTTATGTGAAATAAATACTGG
TTAACGAATGAAAATATCGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPM
LSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKL
QGEGWPSRIEVRGEVFMPKVGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKERRKGDE
NPIVFPTSCPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARH
ITAFFAEEKNQRVVQALIEQGIVWPPVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVF
AGEAAGSKLTKAQELGVEIQTEQDLLALL

Sequences:

>Translated_669_residues
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPM
LSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKL
QGEGWPSRIEVRGEVFMPKVGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQE
ELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKERRKGDE
NPIVFPTSCPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARH
ITAFFAEEKNQRVVQALIEQGIVWPPVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVF
AGEAAGSKLTKAQELGVEIQTEQDLLALL
>Mature_668_residues
SESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPSQRVGGVALDSFRQVTHEIPML
SLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGLAVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQ
GEGWPSRIEVRGEVFMPKVGFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSHY
QRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEKLGFVARAPRWAIAYKFPAQEE
LTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKERRKGDEN
PIVFPTSCPVCHSHVERIEGEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSAG
VLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQTATHEQLIEVPDIGEVVARHI
TAFFAEEKNQRVVQALIEQGIVWPPVEELGSEIPQPLAGKVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFA
GEAAGSKLTKAQELGVEIQTEQDLLALL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=64.9700598802395, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=561, Percent_Identity=22.4598930481283, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73575; Mature: 73444

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPS
CCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCH
QRVGGVALDSFRQVTHEIPMLSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGL
HHHCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCE
AVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQGEGWPSRIEVRGEVFMPKV
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECEEECCCC
GFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCC
NADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKERRKGDENPIVFPTSCPVCHSHVERIE
CHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHC
GEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
CCHHEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TATHEQLIEVPDIGEVVARHITAFFAEEKNQRVVQALIEQGIVWPPVEELGSEIPQPLAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
KVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFAGEAAGSKLTKAQELGVEIQ
EEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCEEE
TEQDLLALL
CCHHHHHCC
>Mature Secondary Structure 
SESKQQYLEELKQQLHYHAVRYYVEDNPEIPDAEYDRMMRELMAIEAEHPEWISVDSPS
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCCH
QRVGGVALDSFRQVTHEIPMLSLDNAFSDEELESFLKRAQDRMPSAHIDAFCCEPKLDGL
HHHCCHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCE
AVSLLYENGVLVQAATRGDGTTGENITENVRTIASVPLKLQGEGWPSRIEVRGEVFMPKV
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECEEECCCC
GFEKLNDIARKKGEKVFVNPRNAAAGSLRQLDSKITATRPLAFYAYSVGVVEGISLSSSH
CHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
YQRFLQLKQWGLPMCPETKLVNGLESVKAFYADILNRRDALPYEIDGVVIKIDDIVIQEK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEHHHHHHHH
LGFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVGGVTVSNATLH
HHHHHCCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCHHHCCCEEECCEEECCCCCC
NADEIERLGVMVGDTVVIRRAGDVIPQIVSVVKERRKGDENPIVFPTSCPVCHSHVERIE
CHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHC
GEAVTRCSGGLVCQAQRKEALKHFVSRKALDVDGLGDKVIEQLVDKEMVETPADLFTLSA
CCHHEECCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
GVLTVLERMGPKSAQNIVNALNVAKETTLARFLYSLGIREVGEATAANLARHFKTLEAIQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TATHEQLIEVPDIGEVVARHITAFFAEEKNQRVVQALIEQGIVWPPVEELGSEIPQPLAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
KVVVLTGTLTQLSRGDAKAALERLGAKVTGSVSKKTDIVFAGEAAGSKLTKAQELGVEIQ
EEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCEEE
TEQDLLALL
CCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA