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The map label for this gene is gcvA [H]

Identifier: 37679062

GI number: 37679062

Start: 879651

End: 880574

Strand: Direct

Name: gcvA [H]

Synonym: VV0878

Alternate gene names: 37679062

Gene position: 879651-880574 (Clockwise)

Preceding gene: 37679060

Following gene: 37679063

Centisome position: 26.22

GC content: 46.97

Gene sequence:

>924_bases
ATGTCCCGAAGATTGCCCCCACTGAATTCCCTTCGTGTATTCGAAGCCGCCGCTAGACATCTTAGTTTCACTCGAGCTGC
AGAAGAGCTCTTTGTCACTCAAGCGGCAGTGAGTCATCAGATCAAAGCACTTGAAGAGTTTCTTTCATTGAAACTGTTCC
GCCGCCGCAACCGTTCTTTACTCCTCACTGAAGAGGGGCAAAGTTACTTTTTAGACATCAAAGATATCTTCTCAGCACTC
GCGGAAGCGACCGATAAGGTATTGGAACGTAGCGAAAAAGGGGCATTGACCATCAGTTTGCCACCGAGTTTTGCCATTCA
ATGGTTGGTACCAAGATTGGCGGATTTCAATGCTAAAGAGCCAGACATTGACGTGCGAATCAAAGCGGTGGATATGGACG
AAGGCTCTTTGACGGACGATGTGGACGTGGCGATTTATTACGGTCGTGGCAATTGGCCGGGGTTACGTGCCGATAAATTG
TACCAAGAATATTTGATCCCACTTTGTTCACCTTCATTACTTTTAGGTAACAAACCATTAGAATCTTTGCAGGATTTAAA
ACGACATACGTTACTCCATGACACCTCCAGAAAAGATTGGAAACAATTTGCTAAGGAAAACGGTCTGGATGAGGTCAATG
TCAACCAAGGTCCGATTTTTAGTCACTCGACCATGGTTCTGCAAGCGGCGGCTCACGGGCAAGGGGTAGCCTTGGGCAAC
AATGTGCTGGCCAAACCAGAAATGGAAGCTGGCCGTTTGATTGCACCCTTTGATGAAATATTGTTGTCGAAGAACTCGTT
TTACGTCGTCTGCCATGAACAGCAAGCTGACATGGGGCGTATCGCGCGATTTCGAGACTGGATGTTAGTGACCGCGCGCA
AAGAACAAGAAGAGGTGTTAGATGAATCTGCCATGCATTATTGA

Upstream 100 bases:

>100_bases
AGGCTAGCGTTGCGGGGTGAAATATAGAATTTATCTGGCTGTTCTACAATGGACAAATTATAATGTTTCAGATTAAAAAA
ACTAATGGTTAGAGAGCCGT

Downstream 100 bases:

>100_bases
TGGGCCAGACAACGGACCGCTGTTTATTTTTGCTCACGGTGCAGGGGCTCCGATGGAGCATGCTTTTATGACCGCAGTGG
CTCAAGGATTGGCAAAGGAA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFSAL
AEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNAKEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWPGLRADKL
YQEYLIPLCSPSLLLGNKPLESLQDLKRHTLLHDTSRKDWKQFAKENGLDEVNVNQGPIFSHSTMVLQAAAHGQGVALGN
NVLAKPEMEAGRLIAPFDEILLSKNSFYVVCHEQQADMGRIARFRDWMLVTARKEQEEVLDESAMHY

Sequences:

>Translated_307_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFSAL
AEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNAKEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWPGLRADKL
YQEYLIPLCSPSLLLGNKPLESLQDLKRHTLLHDTSRKDWKQFAKENGLDEVNVNQGPIFSHSTMVLQAAAHGQGVALGN
NVLAKPEMEAGRLIAPFDEILLSKNSFYVVCHEQQADMGRIARFRDWMLVTARKEQEEVLDESAMHY
>Mature_306_residues
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFSALA
EATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNAKEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWPGLRADKLY
QEYLIPLCSPSLLLGNKPLESLQDLKRHTLLHDTSRKDWKQFAKENGLDEVNVNQGPIFSHSTMVLQAAAHGQGVALGNN
VLAKPEMEAGRLIAPFDEILLSKNSFYVVCHEQQADMGRIARFRDWMLVTARKEQEEVLDESAMHY

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=71.4765100671141, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=34.8275862068965, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=29.8611111111111, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=28.7719298245614, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=27.0833333333333, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081978, Length=272, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=32.484076433121, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789440, Length=276, Percent_Identity=27.1739130434783, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=33.8461538461538, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=262, Percent_Identity=26.7175572519084, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693105, Length=190, Percent_Identity=25.2631578947368, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34768; Mature: 34637

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKDIFSALAEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNAKE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWPGLRADKLYQEYLIPLCSPSLLLGNKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCH
ESLQDLKRHTLLHDTSRKDWKQFAKENGLDEVNVNQGPIFSHSTMVLQAAAHGQGVALGN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEECC
NVLAKPEMEAGRLIAPFDEILLSKNSFYVVCHEQQADMGRIARFRDWMLVTARKEQEEVL
CCCCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEEEECCCHHHHHH
DESAMHY
HHHHCCC
>Mature Secondary Structure 
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKDIFSALAEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNAKE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWPGLRADKLYQEYLIPLCSPSLLLGNKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCH
ESLQDLKRHTLLHDTSRKDWKQFAKENGLDEVNVNQGPIFSHSTMVLQAAAHGQGVALGN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEECCCCCCEEECC
NVLAKPEMEAGRLIAPFDEILLSKNSFYVVCHEQQADMGRIARFRDWMLVTARKEQEEVL
CCCCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEEEECCCHHHHHH
DESAMHY
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]