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The map label for this gene is thiL [H]

Identifier: 37679050

GI number: 37679050

Start: 870328

End: 871332

Strand: Direct

Name: thiL [H]

Synonym: VV0866

Alternate gene names: 37679050

Gene position: 870328-871332 (Clockwise)

Preceding gene: 37679049

Following gene: 37679051

Centisome position: 25.95

GC content: 50.65

Gene sequence:

>1005_bases
ATGCTGGCCTTTTTTATAACAAAGGAAAGGATTTTGATGTCAGGCGAGTTTAATCTGATTGAAAAATACTTCGTTGGCCG
TCAGGCACAACGTAAAGATGTACACCTAGCCGCTGGCGACGACTGCGCGCTAGTTAAGCCACCAAGCAACGTGCAAATTG
CCATTAGCACCGATACCCTAGTATGTGGCACTCATTTCTTAGCCGATGCCGATCCCGCTTGGGTGGCGCATAAGGCGTTA
GCGTCAAACATCAGCGATTTGGCTGCCATGGGCGCAACCCCTGCGTGGGTCTCATTTGCTTTGACTATGCCGCAAGCCGA
CGAAGCCTGGTTGGCTCCCTTTTGTGATGGTTTTTTTAAGCTGGCAGATTACTTCGGCATACAGCTCATTGGTGGTGACA
CGACGAAAGGTCCACTGAGTTTGACCATGACGGTGCAAGGGTTTGTCCCTGAAGGGAAAGCGATCACGCGCAGTGGTGCC
AAACCGGGCGACTATGTGTTTGTTACGGGGACGCTAGGCGATGCAAAGGCGGGATTGGATGTGATTCTCGATAGCAACTT
GCGTACTCGCCCAGCCGCACAAGCGCTAGAAAAAGCGCACTATATGAGTATGCCGCGGATTTTAGCAGGGCAAGCGTTGG
TCGGATTAGCCTCGGCGGCGATCGATATCTCCGATGGCTTAATTGCGGATTTAGGGCACATCTTAAACCGCTCTCAGGTT
GGTGTTAGCCTGGATGTCTCTAAGCTGCCGATTTCATCTGAACTGCTCTCTTTTTTGACCGAGCGCGAAACGGCACTTCA
ATATGCGCTGACCAGCGGCGAAGAATACGAGCTTTGTTTTACCGTGCCCGAAGAGGCAAGAGGAACAATCGAAAGTGCCT
TGGCACACGTTGGTTGCAAAGTGACGTGCATCGGGCAAATTCGTCCACATGGTTTTTTTGAACTCCATGATGATGGCACA
AAATTAAACTGGGATTTGAGTGGCTTTGACCACTTTAAGGCGTAA

Upstream 100 bases:

>100_bases
CGCTGAAGACAGTCACAAGTTTGTCAACGGTGTGCTTGATAAAGCCGCACCGCACGTACGCAAAAAATAAGCGTCACTGA
ATAACGAAAAGGTCAGCACA

Downstream 100 bases:

>100_bases
TCACCATGAACAATCCACTTGATAAAATTTCTTTAAATAATCCTTGGCATTTATTGGCAACGGGGTTTGGCAGTGGCTTA
TCGCCGATTATCCCTGGCAC

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MLAFFITKERILMSGEFNLIEKYFVGRQAQRKDVHLAAGDDCALVKPPSNVQIAISTDTLVCGTHFLADADPAWVAHKAL
ASNISDLAAMGATPAWVSFALTMPQADEAWLAPFCDGFFKLADYFGIQLIGGDTTKGPLSLTMTVQGFVPEGKAITRSGA
KPGDYVFVTGTLGDAKAGLDVILDSNLRTRPAAQALEKAHYMSMPRILAGQALVGLASAAIDISDGLIADLGHILNRSQV
GVSLDVSKLPISSELLSFLTERETALQYALTSGEEYELCFTVPEEARGTIESALAHVGCKVTCIGQIRPHGFFELHDDGT
KLNWDLSGFDHFKA

Sequences:

>Translated_334_residues
MLAFFITKERILMSGEFNLIEKYFVGRQAQRKDVHLAAGDDCALVKPPSNVQIAISTDTLVCGTHFLADADPAWVAHKAL
ASNISDLAAMGATPAWVSFALTMPQADEAWLAPFCDGFFKLADYFGIQLIGGDTTKGPLSLTMTVQGFVPEGKAITRSGA
KPGDYVFVTGTLGDAKAGLDVILDSNLRTRPAAQALEKAHYMSMPRILAGQALVGLASAAIDISDGLIADLGHILNRSQV
GVSLDVSKLPISSELLSFLTERETALQYALTSGEEYELCFTVPEEARGTIESALAHVGCKVTCIGQIRPHGFFELHDDGT
KLNWDLSGFDHFKA
>Mature_334_residues
MLAFFITKERILMSGEFNLIEKYFVGRQAQRKDVHLAAGDDCALVKPPSNVQIAISTDTLVCGTHFLADADPAWVAHKAL
ASNISDLAAMGATPAWVSFALTMPQADEAWLAPFCDGFFKLADYFGIQLIGGDTTKGPLSLTMTVQGFVPEGKAITRSGA
KPGDYVFVTGTLGDAKAGLDVILDSNLRTRPAAQALEKAHYMSMPRILAGQALVGLASAAIDISDGLIADLGHILNRSQV
GVSLDVSKLPISSELLSFLTERETALQYALTSGEEYELCFTVPEEARGTIESALAHVGCKVTCIGQIRPHGFFELHDDGT
KLNWDLSGFDHFKA

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=322, Percent_Identity=54.9689440993789, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 35824; Mature: 35824

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFFITKERILMSGEFNLIEKYFVGRQAQRKDVHLAAGDDCALVKPPSNVQIAISTDTL
CEEEEEECCEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCEEEEEECCEE
VCGTHFLADADPAWVAHKALASNISDLAAMGATPAWVSFALTMPQADEAWLAPFCDGFFK
EECCHHCCCCCHHHHHHHHHHHCHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHH
LADYFGIQLIGGDTTKGPLSLTMTVQGFVPEGKAITRSGAKPGDYVFVTGTLGDAKAGLD
HHHHCCEEEECCCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCCCEE
VILDSNLRTRPAAQALEKAHYMSMPRILAGQALVGLASAAIDISDGLIADLGHILNRSQV
EEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCC
GVSLDVSKLPISSELLSFLTERETALQYALTSGEEYELCFTVPEEARGTIESALAHVGCK
CEEEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTCIGQIRPHGFFELHDDGTKLNWDLSGFDHFKA
EEEEECCCCCCEEEECCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MLAFFITKERILMSGEFNLIEKYFVGRQAQRKDVHLAAGDDCALVKPPSNVQIAISTDTL
CEEEEEECCEEEECCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCEEEEEECCEE
VCGTHFLADADPAWVAHKALASNISDLAAMGATPAWVSFALTMPQADEAWLAPFCDGFFK
EECCHHCCCCCHHHHHHHHHHHCHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHH
LADYFGIQLIGGDTTKGPLSLTMTVQGFVPEGKAITRSGAKPGDYVFVTGTLGDAKAGLD
HHHHCCEEEECCCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCEEEEEECCCCCCCCEE
VILDSNLRTRPAAQALEKAHYMSMPRILAGQALVGLASAAIDISDGLIADLGHILNRSQV
EEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCC
GVSLDVSKLPISSELLSFLTERETALQYALTSGEEYELCFTVPEEARGTIESALAHVGCK
CEEEEHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTCIGQIRPHGFFELHDDGTKLNWDLSGFDHFKA
EEEEECCCCCCEEEECCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]