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The map label for this gene is grpE

Identifier: 37679009

GI number: 37679009

Start: 828463

End: 829059

Strand: Direct

Name: grpE

Synonym: VV0825

Alternate gene names: 37679009

Gene position: 828463-829059 (Clockwise)

Preceding gene: 37679004

Following gene: 37679010

Centisome position: 24.7

GC content: 45.9

Gene sequence:

>597_bases
ATGAGCAACGAAGAAAACAAAATCAACGAAGAAGCGCTTAAACAGCAAGACGCTGCGGAAGTAGAAGTAGAAGCTGTTGG
CACTGATGCTGATATCGAATGGAACGAAGAGGCCGATGAATCAGCGGTGAAGATCGCTGAACTGGAAGCCGCTTTATTAG
CCAGCGAAGCGCGTGTGAAAGAGCAGCAAGATTCAGTGCTACGTGCGAAAGCAGAAGTGGAAAACATGCGTCGCCGTACT
GAGCAAGAAATTGATAAAGCGCGTAAATATGCGTTGAATCGTTTTGCTGAAGAACTGCTGCCAGTGATTGATAATCTAGA
GCGTGCGATTCAAGCGGCTGACGCTGAAAGCGAAGCGGTAAAACCGCTGCTTGAAGGCGTTGAATTGACTCATAAAACGT
TCGTTGATGTGGTCAGTAAGTTTGGTTTGAAAGAAATCAATCCAGAAGGCCAACCATTTAACCCTGAATGGCATCAAGCG
ATGTCGATTCAGGAAAGCCCAGATCACGAGTCAAATACCGTGATGTTCGTGATGCAAAAAGGCTATGAATTGAATGGTCG
AGTGATTCGTCCTGCGATGGTTATGGTTGCGAAATAA

Upstream 100 bases:

>100_bases
ATAATAATGGCAAGTTATCTAACTTTGCGAATTTTTGTGAAGATAAAGCCCATAAAACGTGAAGGGCTTTAAGGACAAAT
AGTTGAATTCTGGAGATATC

Downstream 100 bases:

>100_bases
GCGAACTGATAGACCGATTCAAACACAGAGCGGCTTTTGCCGCTCTTTTTTATGTCTCCAAAGCCCCCTGCACCTAACAA
ACCAACCCTTTGATTTAGTC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAVKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRT
EQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQA
MSIQESPDHESNTVMFVMQKGYELNGRVIRPAMVMVAK

Sequences:

>Translated_198_residues
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAVKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRT
EQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQA
MSIQESPDHESNTVMFVMQKGYELNGRVIRPAMVMVAK
>Mature_197_residues
SNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAVKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRTE
QEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAM
SIQESPDHESNTVMFVMQKGYELNGRVIRPAMVMVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=148, Percent_Identity=35.8108108108108, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=32.4324324324324, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=48.5875706214689, Blast_Score=157, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=34.0425531914894, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=170, Percent_Identity=37.0588235294118, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653432, Length=167, Percent_Identity=34.1317365269461, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_VIBVY (Q7MN92)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_933618.1
- ProteinModelPortal:   Q7MN92
- SMR:   Q7MN92
- STRING:   Q7MN92
- GeneID:   2623603
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0825
- NMPDR:   fig|196600.1.peg.893
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14150
- BioCyc:   VVUL196600:VV0825-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22305; Mature: 22174

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAVKIAELEAALLASEARVK
CCCCCHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQQDSVLRAKAEVENMRRRTEQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAMSIQESPDHESNTVMFVMQK
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEC
GYELNGRVIRPAMVMVAK
CCCCCCEEECHHHHHCCC
>Mature Secondary Structure 
SNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAVKIAELEAALLASEARVK
CCCCHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQQDSVLRAKAEVENMRRRTEQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAMSIQESPDHESNTVMFVMQK
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEEC
GYELNGRVIRPAMVMVAK
CCCCCCEEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA