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The map label for this gene is iscA [H]

Identifier: 37678941

GI number: 37678941

Start: 762380

End: 762703

Strand: Direct

Name: iscA [H]

Synonym: VV0757

Alternate gene names: 37678941

Gene position: 762380-762703 (Clockwise)

Preceding gene: 37678940

Following gene: 37678942

Centisome position: 22.73

GC content: 43.83

Gene sequence:

>324_bases
ATGGCCGTTACATTAACAGATTCCGCAGCAAATCGAGTAAGAGCGTTCCTAGATAACCGAGGCAAAGGCATTGGTTTACG
TTTAGGTGTGAAGACGACTGGTTGTTCGGGTATGGCCTATGTCCTTGAGTTTGTTGACGAACTGAATGAAGAAGACGAAG
TGTTTGAACATTCAGGCGTTAAGGTGATTATCGATAAAAAGAGCTTGGTGTACCTCGACGGCACGCAACTTGACTACGTA
AAAGAAGGGTTGAATGAAGGCTTTGAATTCAACAACCCAAATGCGAAAAGTGAATGTGGTTGCGGTGAAAGCTTCAACGT
CTAA

Upstream 100 bases:

>100_bases
TGACTACAAGAAAAAACACCAACTTTAAGGTTTTTTTTGAGGTTATAATGGGGGCCTTATGCTCCCATTCCATTGGTAAA
CTATTTAACTAAGGTGCAGT

Downstream 100 bases:

>100_bases
TGACTGAAATTCTGAGCCGTTACCGTCGGCTCAGAAGGTTTATTCAAGGACCATAGATCAATGAATCACTTTGAATTGTT
TGGGCTACCACCTCAGTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAVTLTDSAANRVRAFLDNRGKGIGLRLGVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDKKSLVYLDGTQLDYV
KEGLNEGFEFNNPNAKSECGCGESFNV

Sequences:

>Translated_107_residues
MAVTLTDSAANRVRAFLDNRGKGIGLRLGVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDKKSLVYLDGTQLDYV
KEGLNEGFEFNNPNAKSECGCGESFNV
>Mature_106_residues
AVTLTDSAANRVRAFLDNRGKGIGLRLGVKTTGCSGMAYVLEFVDELNEEDEVFEHSGVKVIIDKKSLVYLDGTQLDYVK
EGLNEGFEFNNPNAKSECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=45.2830188679245, Blast_Score=109, Evalue=4e-25,
Organism=Homo sapiens, GI160420328, Length=106, Percent_Identity=36.7924528301887, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=74.7663551401869, Blast_Score=168, Evalue=8e-44,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=47.6190476190476, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=43.6363636363636, Blast_Score=85, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=41.5094339622642, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=41.5094339622642, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=34.9056603773585, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=40, Blast_Score=93, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=43.3962264150943, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=36.1111111111111, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11686; Mature: 11555

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTLTDSAANRVRAFLDNRGKGIGLRLGVKTTGCSGMAYVLEFVDELNEEDEVFEHSGV
CEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCC
KVIIDKKSLVYLDGTQLDYVKEGLNEGFEFNNPNAKSECGCGESFNV
EEEEECCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVTLTDSAANRVRAFLDNRGKGIGLRLGVKTTGCSGMAYVLEFVDELNEEDEVFEHSGV
EEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCC
KVIIDKKSLVYLDGTQLDYVKEGLNEGFEFNNPNAKSECGCGESFNV
EEEEECCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA