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The map label for this gene is queA

Identifier: 37678929

GI number: 37678929

Start: 751666

End: 752718

Strand: Direct

Name: queA

Synonym: VV0745

Alternate gene names: 37678929

Gene position: 751666-752718 (Clockwise)

Preceding gene: 37678928

Following gene: 37678930

Centisome position: 22.41

GC content: 48.62

Gene sequence:

>1053_bases
ATGCAAGTTTCAGATTTTCACTTTGACTTACCTGACGAACTGATCGCTCGTTATCCTCAATCGGAGCGTACCGCCAGTCG
TCTACTTCAGCTAAATGGCAACACCGGCGCTGTAAAAGATGGCTCGTTCAAAGATGTGTTGGAGCTGGTTCAAGCAGGGG
ATCTTGTGGTGTTTAACAACACGCGAGTGATTCCTGCACGCATGTTTGGTCGTAAAGAATCTGGCGGCAAGTTGGAAGTC
TTGGTCGAGCGCATGCTTGATGAGAAGCGCTTCCTCGCGCATGTACGCAGTTCTAAGTCTCCGAAACCGGGTACGTTAGT
CTTTCTTGGGGAAGAAGACCAATACTCAGCAGAGATGGTAGCACGCCAAGATGCGTTGTTTGAGCTCCATTTAAAGGCAG
ACAAAACCATTCTCGAAGTGTTGGAAGAAATTGGCCATATGCCTCTTCCACCTTACATTGACCGCCCAGATGAGGATGCG
GATAAAGAGCGTTACCAAACGGTTTATAACCAAAAACCGGGTGCGGTTGCTGCACCGACGGCGGGATTGCATTTTGACAA
TCAGCTGCTTGAGCAGATCAAAGCCAAAGGCGCTGAGTTTGCGTACGTGACGTTGCATGTCGGTGCTGGCACATTTCAGC
CAGTGAAAGTGGACAATATTCTTGAGCATCACATGCACTCTGAGTATGCAGAAGTACCACAAGAGGTGGTGGATGCGATT
AAAGCCACGAAAGCACGTGGTGGCCGCGTCATTGCGGTTGGTACCACGTCAGTACGCTCTCTAGAGAGCGCGGCGCAAGA
GTCGTTGAAAAACGGCACTGAGTTGATGCCTTTCTTTGGCGATACGGAAATCTTCATTTTCCCTGGCTATCAATATCAGT
TGGTGGACTGTTTGATCACCAATTTCCATTTGCCAGAATCGACGCTGATTATGCTGGTGAGTGCGTTTGCCGGTTATGAC
CATACAATGAATGCGTATCAGCATGCCGTGGCCAATCAATATCGCTTTTTCTCTTACGGGGATGCGATGTTCATTGAGAA
GAAAACGCAATAA

Upstream 100 bases:

>100_bases
TTTCTTGCTATTGACTGGTCTCGCTCTATACTAGCCCACTGCGAAAATTTTACTCGCAACAGAACTCCTTTATTTCGACA
AATTTACCAAGAGCAGTAGC

Downstream 100 bases:

>100_bases
GCAATAAAAAGCGCAACAAATGTTATCTAAAGTGAAGTAAGTTTCATTTTAGTGGTGCAGATCGGGATAATCTTGGCTGC
ATGCAAAACGAGTGCTAGCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLVVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
KATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVANQYRFFSYGDAMFIEKKTQ

Sequences:

>Translated_350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLVVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
KATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVANQYRFFSYGDAMFIEKKTQ
>Mature_350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLVVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
KATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVANQYRFFSYGDAMFIEKKTQ

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=68.2857142857143, Blast_Score=507, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39207; Mature: 39207

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLVVFNN
CCCCCCCCCCCHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGRKESGGKLEVLVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMV
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
ARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDADKERYQTVYNQKPGAVAAPT
HHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLIT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECEEECCCEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYDHTMNAYQHAVANQYRFFSYGDAMFIEKKTQ
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCEEEEEECCC
>Mature Secondary Structure
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLVVFNN
CCCCCCCCCCCHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGRKESGGKLEVLVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMV
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
ARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDADKERYQTVYNQKPGAVAAPT
HHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLIT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECEEECCCEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYDHTMNAYQHAVANQYRFFSYGDAMFIEKKTQ
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA