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The map label for this gene is thyA

Identifier: 37678863

GI number: 37678863

Start: 676928

End: 677779

Strand: Direct

Name: thyA

Synonym: VV0679

Alternate gene names: 37678863

Gene position: 676928-677779 (Clockwise)

Preceding gene: 37678862

Following gene: 37678866

Centisome position: 20.18

GC content: 47.42

Gene sequence:

>852_bases
GTGAAACAATATTTAGAATTGTGCCGTCGCATCGTTGATGAAGGGCACTGGGTTGAAAATGAACGTACCGGTAAGCGCTG
CCTTACTGTGATCAACGCGGATTTAACCTACGATGTTGCCAACAATCAGTTTCCTTTGGTTACCACGCGTAAGAGTTTCT
GGAAGGCGGCGGTTGCAGAGTTGCTTGGCTACATTCGAGGCTATGACAATGCGGAAGATTTTCGCAAGTTAGGTACGAAA
ACCTGGGATGCGAACGCCAATCTCAATGATGCTTGGTTGAATAACCCATATCGCAAAGGCGATGATGACATGGGCCGTGT
CTACGGCGTACAAGGTCGAGCTTGGGCGAAGCCTGATGGTGGTCATATCGACCAATTGCGTAAAATTGTCGATGACCTGA
CGCGCGGAGTAGACGATCGCGGGGAGATCCTCAATTTCTACAACCCAGGTGAATTTCATATGGGCTGTTTACGCCCTTGT
ATGTACAGTCATCACTTCTCATTGCTGGGTGATACGTTATACCTCAATAGCACACAACGTTCGTGTGACGTACCGCTGGG
GCTGAATTTCAACATGGTCCAGGTGTATGTGTTCTTGGCGATCATGGCACAGATTACGGGCAAGAAACCGGGCCAAGCAT
TCCATAAGATTGTTAATGCACATATCTATGAAGATCAGTTAGCACTGATGCGTGATGTGCAACTCAAGCGAGAACCGTTA
CAAGCGCCAACGTTCCACATCAACCCTGAGATCAAATCTTTGGAAGATCTGGAAACTTGGGTCACTTTGGATGATTTCTG
GGTAGAAGGGTATGAACATCATGACCCAATCCAGTACCCATTCTCGGTATAA

Upstream 100 bases:

>100_bases
CTTGTCGTCACCGATGATTATTGGTGGATTGGCGCTGATGGCTTGGGCTTATAAACGTGGCCACTATCAAGACAAAGTAA
CCGTGAAGTAAGGAATTGAA

Downstream 100 bases:

>100_bases
CATCACGATGGCTGAAAAGGGCTCCTAGAGCCCTTTTTTGTTACCTACAGCGTTTGTGCTATTTGAGCGCATTGGCGACA
AATTTATAGATAGGATCCGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLELCRRIVDEGHWVENERTGKRCLTVINADLTYDVANNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANANLNDAWLNNPYRKGDDDMGRVYGVQGRAWAKPDGGHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKPGQAFHKIVNAHIYEDQLALMRDVQLKREPL
QAPTFHINPEIKSLEDLETWVTLDDFWVEGYEHHDPIQYPFSV

Sequences:

>Translated_283_residues
MKQYLELCRRIVDEGHWVENERTGKRCLTVINADLTYDVANNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANANLNDAWLNNPYRKGDDDMGRVYGVQGRAWAKPDGGHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKPGQAFHKIVNAHIYEDQLALMRDVQLKREPL
QAPTFHINPEIKSLEDLETWVTLDDFWVEGYEHHDPIQYPFSV
>Mature_283_residues
MKQYLELCRRIVDEGHWVENERTGKRCLTVINADLTYDVANNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANANLNDAWLNNPYRKGDDDMGRVYGVQGRAWAKPDGGHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKPGQAFHKIVNAHIYEDQLALMRDVQLKREPL
QAPTFHINPEIKSLEDLETWVTLDDFWVEGYEHHDPIQYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=31.4878892733564, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=32.5088339222615, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71993377, Length=275, Percent_Identity=27.6363636363636, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=26.0726072607261, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137556, Length=290, Percent_Identity=28.6206896551724, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32703; Mature: 32703

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLELCRRIVDEGHWVENERTGKRCLTVINADLTYDVANNQFPLVTTRKSFWKAAVAE
CHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCEEEEECCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAEDFRKLGTKTWDANANLNDAWLNNPYRKGDDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLAIMAQITGKKPGQAFHKIVNAHIYEDQLALMRDVQLKREPL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QAPTFHINPEIKSLEDLETWVTLDDFWVEGYEHHDPIQYPFSV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLELCRRIVDEGHWVENERTGKRCLTVINADLTYDVANNQFPLVTTRKSFWKAAVAE
CHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCEEEEECCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAEDFRKLGTKTWDANANLNDAWLNNPYRKGDDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLRKIVDDLTRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLAIMAQITGKKPGQAFHKIVNAHIYEDQLALMRDVQLKREPL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QAPTFHINPEIKSLEDLETWVTLDDFWVEGYEHHDPIQYPFSV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA