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The map label for this gene is nfo [H]

Identifier: 37678838

GI number: 37678838

Start: 650641

End: 651522

Strand: Direct

Name: nfo [H]

Synonym: VV0654

Alternate gene names: 37678838

Gene position: 650641-651522 (Clockwise)

Preceding gene: 37678836

Following gene: 37678839

Centisome position: 19.4

GC content: 45.69

Gene sequence:

>882_bases
ATGACAAACAGCAAAAACCGTTTTGGTAACAAATTTATTGGCGCGCATGTTTCAGCGGCGGGCGGGGTTGACAATGCACC
TCTGCGGGCGCGTGAGATTGGAGCAAACGCGTTTGCTTTGTTCACGAAGAATCAACGTCAATGGGTTGCCAAGCCATTAG
AAGAGAAAACCATCTCGGCGTTCAAAGCCAATTGCGCGTTGTTGGGTTTTTCTCCACAGCAAATTCTTCCTCATGATTCT
TATCTTATTAATTTAGGTGCGCCAGAAGCGGAGAAGCTGGAAAAGTCTCGTCTCGCGTTTATTGATGAAATGGAGCGATG
CCAGCTGCTAGGGTTGAACTTACTCAATTTCCACCCAGGCAGCCATTTGGAGAAGATCTCAGAAAAAGCGTGTTTGTATT
TAATCGCAGAATCGATCAACTTAGCGCATCAAGCGGTACCTGATGTGGTGGCGGTGATAGAAAACACGGCCGGACAAGGT
TCCAACTTAGGTTGGCGATTTGAGCACCTTGCAGAAATCATTGAGCAGGTTGAAGATAAGAGTCGAGTTGGGGTTTGTTT
GGATACTTGCCACACCTTTGCCGCAGGCTATGATCTGCGAACTCCTGAGGCGTGTGAGGCGACCTTCGCTGAGTTTGAAC
GTGTGGTCGGAATGCACTATTTACGTGCAATGCATATTAATGATTCAAAAGTAAAATTAGCCAGTAAGGTGGATAGGCAC
CATGCCCTAGGGAAAGGTGAAATTGGCTGGGACTGTTTTGAATACATTGCGAAAGACAGCCGTTTTGATTCTATCCCATT
GATTTTAGAAACTATTGAACCTGAACTTTGGCCGCAAGAAATTGAGCAGTTGAGAAAATATCATCTCTCTTCAATCGCTT
AG

Upstream 100 bases:

>100_bases
AATTTTGTTGTAAATTTACTACAAAGTTAATTGGAAATTTGACTGAACTAAGCTAAATGGTTGTAATTAGCCCCTTATCT
TGAAAGTGTTAAGTGATTAA

Downstream 100 bases:

>100_bases
CGCTTTTTGTCATCAGATTGGCATCTTTCTTTCATAGCAAACAAGAACAATAGTGTTTATCGAGTTTGTCACAGGAGGAT
GCCACTATGTTTACAACCAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDS
YLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLYLIAESINLAHQAVPDVVAVIENTAGQG
SNLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACEATFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA

Sequences:

>Translated_293_residues
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDS
YLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLYLIAESINLAHQAVPDVVAVIENTAGQG
SNLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACEATFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
>Mature_292_residues
TNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISAFKANCALLGFSPQQILPHDSY
LINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPGSHLEKISEKACLYLIAESINLAHQAVPDVVAVIENTAGQGS
NLGWRFEHLAEIIEQVEDKSRVGVCLDTCHTFAAGYDLRTPEACEATFAEFERVVGMHYLRAMHINDSKVKLASKVDRHH
ALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=67.6363636363636, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17531193, Length=272, Percent_Identity=48.1617647058824, Blast_Score=275, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=260, Percent_Identity=44.6153846153846, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32726; Mature: 32594

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISA
CCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHH
FKANCALLGFSPQQILPHDSYLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPG
HHCCEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
SHLEKISEKACLYLIAESINLAHQAVPDVVAVIENTAGQGSNLGWRFEHLAEIIEQVEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SRVGVCLDTCHTFAAGYDLRTPEACEATFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
HCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNSKNRFGNKFIGAHVSAAGGVDNAPLRAREIGANAFALFTKNQRQWVAKPLEEKTISA
CCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHCCHHHHHHHH
FKANCALLGFSPQQILPHDSYLINLGAPEAEKLEKSRLAFIDEMERCQLLGLNLLNFHPG
HHCCEEEECCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCC
SHLEKISEKACLYLIAESINLAHQAVPDVVAVIENTAGQGSNLGWRFEHLAEIIEQVEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SRVGVCLDTCHTFAAGYDLRTPEACEATFAEFERVVGMHYLRAMHINDSKVKLASKVDRH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HALGKGEIGWDCFEYIAKDSRFDSIPLILETIEPELWPQEIEQLRKYHLSSIA
HCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA