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The map label for this gene is dhfrIII [H]

Identifier: 37678659

GI number: 37678659

Start: 459740

End: 460228

Strand: Direct

Name: dhfrIII [H]

Synonym: VV0475

Alternate gene names: 37678659

Gene position: 459740-460228 (Clockwise)

Preceding gene: 37678658

Following gene: 37678666

Centisome position: 13.71

GC content: 46.83

Gene sequence:

>489_bases
ATGATCATCAGTATGATTGCCGCGATGGCAAAGCAAAGAATCATTGGTAAAGATAACCAGATGCCTTGGCATCTTCCCGC
AGATTTTGCTTGGTTCAAACGCTGCACGATGGGGAAACCGGTCGTGATGGGGCGTAAAACGTATGATTCGATCGGACGTC
CGTTGCCAGGGCGATTGAATATAGTGATTAGTCGTGACGAAAGCTTGGTTATTGAGGGGGTCACTATGGCCAACTCGATT
GCGCATGCCTTAGCGTTAGCGGGGGATGCAGATGAAGTGATGATCATCGGTGGCGGTTCAATTTACGCTGAGTGTTTACC
AAAAGCAGACAAACTCTATCTGACGTTTATCGATGCCACTATTGATGGCGATACTCAGTTTCCTGATTGGGGGGAGCAAT
GGTGCGAGAGCCATCGCGAACACTATTCTGCTGATGAGAAAAATCGCTACGCGATGGATTTTGTTATTTTAGAAAGAGAT
AATTGCTAG

Upstream 100 bases:

>100_bases
CTTTTTAAAAGCGATGTTCATCATTGCGGGACTGGCGGTTGGCCTTGCCGTTCCTGGGCTGTTATTCTACCGCCGCAGAC
CAATAGTGTAAGGAAGAGAG

Downstream 100 bases:

>100_bases
TTTGCTAGTTTGCTAGTTTGCTAGTTTGCTAGTTTGCTAGTTTGCTAGTTTGCTAGTTTGCTAGTTTTCTAGTTTTCTAG
TTTTCTAGTTTTCTAGTTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MIISMIAAMAKQRIIGKDNQMPWHLPADFAWFKRCTMGKPVVMGRKTYDSIGRPLPGRLNIVISRDESLVIEGVTMANSI
AHALALAGDADEVMIIGGGSIYAECLPKADKLYLTFIDATIDGDTQFPDWGEQWCESHREHYSADEKNRYAMDFVILERD
NC

Sequences:

>Translated_162_residues
MIISMIAAMAKQRIIGKDNQMPWHLPADFAWFKRCTMGKPVVMGRKTYDSIGRPLPGRLNIVISRDESLVIEGVTMANSI
AHALALAGDADEVMIIGGGSIYAECLPKADKLYLTFIDATIDGDTQFPDWGEQWCESHREHYSADEKNRYAMDFVILERD
NC
>Mature_162_residues
MIISMIAAMAKQRIIGKDNQMPWHLPADFAWFKRCTMGKPVVMGRKTYDSIGRPLPGRLNIVISRDESLVIEGVTMANSI
AHALALAGDADEVMIIGGGSIYAECLPKADKLYLTFIDATIDGDTQFPDWGEQWCESHREHYSADEKNRYAMDFVILERD
NC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=147, Percent_Identity=36.0544217687075, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=52.8301886792453, Blast_Score=171, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=27.7372262773723, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=31.0734463276836, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=31.0734463276836, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18185; Mature: 18185

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISMIAAMAKQRIIGKDNQMPWHLPADFAWFKRCTMGKPVVMGRKTYDSIGRPLPGRLN
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCHHHHCCCCCCCEE
IVISRDESLVIEGVTMANSIAHALALAGDADEVMIIGGGSIYAECLPKADKLYLTFIDAT
EEEECCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCEEEEEEEEEE
IDGDTQFPDWGEQWCESHREHYSADEKNRYAMDFVILERDNC
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MIISMIAAMAKQRIIGKDNQMPWHLPADFAWFKRCTMGKPVVMGRKTYDSIGRPLPGRLN
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCHHHHCCCCCCCEE
IVISRDESLVIEGVTMANSIAHALALAGDADEVMIIGGGSIYAECLPKADKLYLTFIDAT
EEEECCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCEEEEEEEEEE
IDGDTQFPDWGEQWCESHREHYSADEKNRYAMDFVILERDNC
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]