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The map label for this gene is mdh [H]

Identifier: 37678651

GI number: 37678651

Start: 454129

End: 455061

Strand: Direct

Name: mdh [H]

Synonym: VV0467

Alternate gene names: 37678651

Gene position: 454129-455061 (Clockwise)

Preceding gene: 37678646

Following gene: 37678654

Centisome position: 13.54

GC content: 48.55

Gene sequence:

>933_bases
ATGAAAGTAGCTGTTATTGGTGCCGCTGGTGGCATCGGTCAAGCCCTAGCTCTTTTACTGAAAAACCGCCTTCCAGCGGG
TTCTGATTTAGCCCTATATGATATTGCTCCGGTTACTCCGGGTGTTGCTGCAGACCTAAGCCACATCCCAACCCCCGTTT
CGATCAAAGGTTATGCCGGTGAAGATCCAACGCCAGCACTAGAAGGTGCTGATGTGGTTCTTATTTCTGCCGGTGTTGCG
CGTAAGCCAGGTATGGATCGTGCGGATCTATTTAATGTTAATGCTGGTATCGTCAAATCACTTGCTGAGCGCATTGCCGT
GGTTTGTCCAAATGCTTGTATTGGTATCATCACCAACCCTGTGAACACCACTGTGCCAATTGCAGCAGAAGTTCTGAAAA
AAGCGGGCGTTTATGACAAGCGTAAACTTTTTGGTGTGACGACGTTGGATGTGATCCGTTCAGAAACGTTTGTTGCTGAG
CTGAAAGGTCAAGATCCAGGTGAAGTTCGTGTACCAGTGATCGGCGGTCACTCTGGTGTGACGATTCTGCCTCTTCTTTC
TCAAGTGGAAGGTGTCGAGTTCAGCGACGAAGAAATCGCAGCCCTAACCAAACGTATCCAAAATGCAGGTACTGAAGTGG
TTGAAGCGAAAGCGGGTGGCGGTAGTGCAACACTTTCAATGGGTCAGGCTGCGTGTCGTTTTGGTCTTGCGCTCGTGAAA
GCACTGCAAGGTGAAGAAGTGATCGAATACGCTTACGTTGAAGGTAACGGCGAGCATGCTAGCTTCTTTGCACAGCCAGT
GAAACTTGGCAAAGAGGGTGTTGAAGAAATCCTACCTTATGGCGAGCTAAGTGATTTCGAAAAAGCAGCGCTAGATGGCA
TGCTAGAAACGCTTAACGGTGACATTCAAATTGGCGTGGACTTCGTAAAGTAA

Upstream 100 bases:

>100_bases
TGATTGTAATCAAGTTGTAATTGCTATAGCGTTCTACACAGTTGTTTACAAAATACCCTACTAAAACGTCTATAAGAGAT
CGCTCTCAAGGAGAACTACA

Downstream 100 bases:

>100_bases
GTCAGCGAATGTGAGTGTTAGAAAAACAAAGCCGATCGTTGGATCGGCTTTTTTGTTTGTGGTTGATAGTATTCGACTCA
ACAAAGAAGAAAGGGAGCAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAERIAVVCPNACIGIITNPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAE
LKGQDPGEVRVPVIGGHSGVTILPLLSQVEGVEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEEVIEYAYVEGNGEHASFFAQPVKLGKEGVEEILPYGELSDFEKAALDGMLETLNGDIQIGVDFVK

Sequences:

>Translated_310_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAERIAVVCPNACIGIITNPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAE
LKGQDPGEVRVPVIGGHSGVTILPLLSQVEGVEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEEVIEYAYVEGNGEHASFFAQPVKLGKEGVEEILPYGELSDFEKAALDGMLETLNGDIQIGVDFVK
>Mature_310_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAERIAVVCPNACIGIITNPVNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAE
LKGQDPGEVRVPVIGGHSGVTILPLLSQVEGVEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEEVIEYAYVEGNGEHASFFAQPVKLGKEGVEEILPYGELSDFEKAALDGMLETLNGDIQIGVDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=56.4102564102564, Blast_Score=326, Evalue=1e-89,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=78.4565916398714, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17554310, Length=312, Percent_Identity=57.3717948717949, Blast_Score=329, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=52.0634920634921, Blast_Score=278, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=39.9408284023669, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324446, Length=365, Percent_Identity=37.5342465753425, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=57.741935483871, Blast_Score=324, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=50.4792332268371, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=46, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32098; Mature: 32098

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRADLFNVNAGIVKSLAERIAVVCPNACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCCCEEEEEECC
VNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAELKGQDPGEVRVPVIGGHSGV
CCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCC
TILPLLSQVEGVEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGEEVIEYAYVEGNGEHASFFAQPVKLGKEGVEEILPYGELSDFEKAALDGMLETLNG
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DIQIGVDFVK
CEEEEEEECC
>Mature Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRADLFNVNAGIVKSLAERIAVVCPNACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCCCEEEEEECC
VNTTVPIAAEVLKKAGVYDKRKLFGVTTLDVIRSETFVAELKGQDPGEVRVPVIGGHSGV
CCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCC
TILPLLSQVEGVEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGEEVIEYAYVEGNGEHASFFAQPVKLGKEGVEEILPYGELSDFEKAALDGMLETLNG
HHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
DIQIGVDFVK
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA