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The map label for this gene is thiP

Identifier: 37678622

GI number: 37678622

Start: 429038

End: 430609

Strand: Direct

Name: thiP

Synonym: VV0438

Alternate gene names: 37678622

Gene position: 429038-430609 (Clockwise)

Preceding gene: 161486667

Following gene: 37678623

Centisome position: 12.79

GC content: 52.16

Gene sequence:

>1572_bases
ATGATCATCGCGACCTTTGTTGTTTCTGCTCTCTCGGCGCTCTTGTTGGCGACGCCGACGCTGGATCTCACGGCGGTATG
GCAAGATCCTTATTACCGCCATGTCACGCAATTTAGTTTTCTTCAAGCGACGCTGTCGATGCTGCTGAGTGTCGGTTTGG
CGCTTCCTGTCGCACACGCCTTGTCTCGGCGACATTTTGTTGGCCGCTCGCTATTGCTCAAATTGTTTGCGTCAACTTTA
GTGCTGCCAGTATTGGTAGGCGTGTTTGGCTTGTTGGCCATTTATGGGAACAGTGGTTGGTTGGCAAGTGTATTGGCGAG
CTTTGACGTCAAATTGCCCTTTTCGATCTACGGATTGAGCGGCATTTTGCTCGCGCACGTGTTTTTCAATTTGCCGTATG
CAGCGCGATTGCTGCTGCAAACCTTAGAGTCGATTCCGGCAGAGCAGCACAAATTGGCGGCTCATCTTGGTATGAACAGC
TGGCAGAAGTTTCGTTGGGTCGAATGGCCGCGATTGCGCCAGCAACTGCCACATGTGTGTGGCTTGGTGTTCATGCTTTG
CTTCACCAGCTTTGCCACGGTCATGGCCCTCGGTGGCGGCCCAAAATCGACCACCATCGAGCTGGCTATCTACCAAGCGA
TAAAATTTGATTTTGACTTACAAGCTGGAGCGCTACTGGCGATCTGGCAAATGCTATTGTGTAGTTTGCTGGTGTTGGCG
CTGCAGCGATTGACTAAACCGCTGCCTGTCTCGGTTGGCAGCCACACCCGTTTCGTTCAGTTAACCCCAGCTTCTAAGCG
TCATTTGATGTGGGATTATGCGTGGATAGCACTGGCGACACTGCTGGTGGTTCCGCCGCTGCTGTTTGTGGTGGCCAGCG
GGATAAACAGCAAGCTCCCTGACGTGATCAGTGATGGTCGATTTTGGTCCGCTCTGGGGGCCTCGTTACGTGTAGCGTTT
GGCGCCGCCACGTTAGCCGTGGGCGCGGCGATTGCTATTTTGCTCACCAGTCGTGCATGGCGGTTGCGTGGTCTGCGATA
CCCTGCAGACGGCATTGAGATGGTGGGCACCATCATCTTGGTGACGCCAGGTTTGGTGGTGAGTACGGGGCTGTTTTTAT
TGCTGCGCAGTTTTGCAGATGTGTTCAGTTTGTCGCTGTTGATCGTCATCATCGTCAACAGTTTGATGGCATTACCGTAC
GCCATTAAAACGCTCGCTCAGCCGATGTTGCATTGCGAGCAGCAGTATCAATACTTGGCTGCTACATTGGGCTTAAGCGG
GTGGAATCGTTTTAAAATCGTTGAGTGGCGAGCACTGCGTAAACCTTTCGCCAATGCGTTTGCGCTCTGTTTTATGTTTT
CCATTGGCGATTTGAGCGCTATTGCCTTATTTGGTAGCCAAGATTTTCGTACTCTACCGCTCTATCTGTACCAGTTACTC
GGCAGTTATCAAATGGAGGCAGCAGCGGTGGTGTCACTCACGTTATTGCTGCTGAGTGTCGGCTGTTTTGCCCTGAGTGA
GTCCTTACTCAGCAGCAAATCGAATCAACATCAGGAGAAGGCGCCATGCTAA

Upstream 100 bases:

>100_bases
GGCGAAGCAGCGTAAAGCTTGGATTCGCGAATGGCAAAGCGCGTTGACGTTTTAAGGCGTCGCATTGAATTCGGTTCCTA
AATTGGGCATTGGGGTCGCG

Downstream 100 bases:

>100_bases
CGCTGCAACAGGTGCATTATTATTATCATCAAGATCTGTTTGCTTTTGATTTAGAGGTAGAAGCAGGCAGTATTGTGGCG
TTGATGGGGCCAAGTGGGGC

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MIIATFVVSALSALLLATPTLDLTAVWQDPYYRHVTQFSFLQATLSMLLSVGLALPVAHALSRRHFVGRSLLLKLFASTL
VLPVLVGVFGLLAIYGNSGWLASVLASFDVKLPFSIYGLSGILLAHVFFNLPYAARLLLQTLESIPAEQHKLAAHLGMNS
WQKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLAIWQMLLCSLLVLA
LQRLTKPLPVSVGSHTRFVQLTPASKRHLMWDYAWIALATLLVVPPLLFVVASGINSKLPDVISDGRFWSALGASLRVAF
GAATLAVGAAIAILLTSRAWRLRGLRYPADGIEMVGTIILVTPGLVVSTGLFLLLRSFADVFSLSLLIVIIVNSLMALPY
AIKTLAQPMLHCEQQYQYLAATLGLSGWNRFKIVEWRALRKPFANAFALCFMFSIGDLSAIALFGSQDFRTLPLYLYQLL
GSYQMEAAAVVSLTLLLLSVGCFALSESLLSSKSNQHQEKAPC

Sequences:

>Translated_523_residues
MIIATFVVSALSALLLATPTLDLTAVWQDPYYRHVTQFSFLQATLSMLLSVGLALPVAHALSRRHFVGRSLLLKLFASTL
VLPVLVGVFGLLAIYGNSGWLASVLASFDVKLPFSIYGLSGILLAHVFFNLPYAARLLLQTLESIPAEQHKLAAHLGMNS
WQKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLAIWQMLLCSLLVLA
LQRLTKPLPVSVGSHTRFVQLTPASKRHLMWDYAWIALATLLVVPPLLFVVASGINSKLPDVISDGRFWSALGASLRVAF
GAATLAVGAAIAILLTSRAWRLRGLRYPADGIEMVGTIILVTPGLVVSTGLFLLLRSFADVFSLSLLIVIIVNSLMALPY
AIKTLAQPMLHCEQQYQYLAATLGLSGWNRFKIVEWRALRKPFANAFALCFMFSIGDLSAIALFGSQDFRTLPLYLYQLL
GSYQMEAAAVVSLTLLLLSVGCFALSESLLSSKSNQHQEKAPC
>Mature_523_residues
MIIATFVVSALSALLLATPTLDLTAVWQDPYYRHVTQFSFLQATLSMLLSVGLALPVAHALSRRHFVGRSLLLKLFASTL
VLPVLVGVFGLLAIYGNSGWLASVLASFDVKLPFSIYGLSGILLAHVFFNLPYAARLLLQTLESIPAEQHKLAAHLGMNS
WQKFRWVEWPRLRQQLPHVCGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLAIWQMLLCSLLVLA
LQRLTKPLPVSVGSHTRFVQLTPASKRHLMWDYAWIALATLLVVPPLLFVVASGINSKLPDVISDGRFWSALGASLRVAF
GAATLAVGAAIAILLTSRAWRLRGLRYPADGIEMVGTIILVTPGLVVSTGLFLLLRSFADVFSLSLLIVIIVNSLMALPY
AIKTLAQPMLHCEQQYQYLAATLGLSGWNRFKIVEWRALRKPFANAFALCFMFSIGDLSAIALFGSQDFRTLPLYLYQLL
GSYQMEAAAVVSLTLLLLSVGCFALSESLLSSKSNQHQEKAPC

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=477, Percent_Identity=51.1530398322851, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788764, Length=201, Percent_Identity=27.363184079602, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57276; Mature: 57276

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATFVVSALSALLLATPTLDLTAVWQDPYYRHVTQFSFLQATLSMLLSVGLALPVAHA
CCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRRHFVGRSLLLKLFASTLVLPVLVGVFGLLAIYGNSGWLASVLASFDVKLPFSIYGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
GILLAHVFFNLPYAARLLLQTLESIPAEQHKLAAHLGMNSWQKFRWVEWPRLRQQLPHVC
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
GLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLAIWQMLLCSLLVLA
HHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LQRLTKPLPVSVGSHTRFVQLTPASKRHLMWDYAWIALATLLVVPPLLFVVASGINSKLP
HHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVISDGRFWSALGASLRVAFGAATLAVGAAIAILLTSRAWRLRGLRYPADGIEMVGTIIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VTPGLVVSTGLFLLLRSFADVFSLSLLIVIIVNSLMALPYAIKTLAQPMLHCEQQYQYLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGLSGWNRFKIVEWRALRKPFANAFALCFMFSIGDLSAIALFGSQDFRTLPLYLYQLL
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHH
GSYQMEAAAVVSLTLLLLSVGCFALSESLLSSKSNQHQEKAPC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIIATFVVSALSALLLATPTLDLTAVWQDPYYRHVTQFSFLQATLSMLLSVGLALPVAHA
CCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRRHFVGRSLLLKLFASTLVLPVLVGVFGLLAIYGNSGWLASVLASFDVKLPFSIYGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
GILLAHVFFNLPYAARLLLQTLESIPAEQHKLAAHLGMNSWQKFRWVEWPRLRQQLPHVC
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
GLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAGALLAIWQMLLCSLLVLA
HHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LQRLTKPLPVSVGSHTRFVQLTPASKRHLMWDYAWIALATLLVVPPLLFVVASGINSKLP
HHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVISDGRFWSALGASLRVAFGAATLAVGAAIAILLTSRAWRLRGLRYPADGIEMVGTIIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VTPGLVVSTGLFLLLRSFADVFSLSLLIVIIVNSLMALPYAIKTLAQPMLHCEQQYQYLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGLSGWNRFKIVEWRALRKPFANAFALCFMFSIGDLSAIALFGSQDFRTLPLYLYQLL
HHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHH
GSYQMEAAAVVSLTLLLLSVGCFALSESLLSSKSNQHQEKAPC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]