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The map label for this gene is tbpA

Identifier: 161486667

GI number: 161486667

Start: 427985

End: 428992

Strand: Direct

Name: tbpA

Synonym: VV0437

Alternate gene names: 161486667

Gene position: 427985-428992 (Clockwise)

Preceding gene: 37678620

Following gene: 37678622

Centisome position: 12.76

GC content: 50.2

Gene sequence:

>1008_bases
GTGAAACACACTCTAAACCTGATAGCCCTAGCGTCAATCACGTCAATGATGGGCATCTCTTCTTCTGCACTTGCGGCGGA
CAAAACCTTAACCATTTACACCTACGATTCTTTTGCCTCCGATTGGGGTCCTGGCCCGACAGTCGAAAAAGCATTTGAAG
CTCAGTGCGGCTGCGATGTGAATTTTGTCGCGCTGGACGATGGGGTGTCAATTCTCAACCGCTTACGTTTAGAAGGCAGC
AATACCAAAGCGGATATTGTTTTAGGTTTGGATAACAATTTGATGGCAGAAGCCAAAGCCACAGGGCTGCTTGCGCAGCA
TCAAGTAGAAACCACGGCTATTACGCTGCCGAATGGTTGGGCGGATGATACGTTTATTCCTTATGACTACGGTTACTTTG
CGTTTGTTTACAACAAAGGCAAACTGGCGAATCCACCCAAAAGCCTAAAAGAGCTGGTGGAATCACGCGACGATCTGAAA
GTCATCTACCAAGACCCTCGCACTTCAACGCCCGGTCAAGGTTTGATGCTGTGGATGAAGTCGGTGTATGGCGATGACGT
GACTGGAGCATGGCAGCAACTGGCGAAGAAAACCGTGACGGTCACGAAAGGTTGGTCGGAAGCGTATTCGATGTTCCTCA
AAGGCGAATCGGACATGGTGCTCTCTTACACCACCTCACCTGCGTACCACCTGATTGCCGAAAATGATGCACAATATGCC
GCGGCGGGTTTTAGTGAAGGGCACTACATGCAAGTGGAAGTCGCCGCAAAAGTGAAAAGTAGCCAGAACTCTGCGCTGGC
TGATCAGTTTATGAGTTTCATTTTGAGTGATGAGTTCCAAAGCGCAATGCCTACGGGCAACTGGATGTACCCAGTGACCA
ACGTGACGCTGCCAGAGGGCTACCGCAGTTTAACCGTGCCTGAGAAGGCGTTGAGTTTCAGTGCGGAAGAGGTGGCGAAG
CAGCGTAAAGCTTGGATTCGCGAATGGCAAAGCGCGTTGACGTTTTAA

Upstream 100 bases:

>100_bases
TAATGCTGGCGTAGGAATTGAGTCGGGATTAATTAGCAAGGACGATTAACACCTACCTCAAGCCTGTGCCGCTCAACTCA
TGCCAAATGGAGAGCGAGCA

Downstream 100 bases:

>100_bases
GGCGTCGCATTGAATTCGGTTCCTAAATTGGGCATTGGGGTCGCGATGATCATCGCGACCTTTGTTGTTTCTGCTCTCTC
GGCGCTCTTGTTGGCGACGC

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDVNFVALDDGVSILNRLRLEGS
NTKADIVLGLDNNLMAEAKATGLLAQHQVETTAITLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLK
VIYQDPRTSTPGQGLMLWMKSVYGDDVTGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAENDAQYA
AAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEGYRSLTVPEKALSFSAEEVAK
QRKAWIREWQSALTF

Sequences:

>Translated_335_residues
MKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDVNFVALDDGVSILNRLRLEGS
NTKADIVLGLDNNLMAEAKATGLLAQHQVETTAITLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLK
VIYQDPRTSTPGQGLMLWMKSVYGDDVTGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAENDAQYA
AAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEGYRSLTVPEKALSFSAEEVAK
QRKAWIREWQSALTF
>Mature_335_residues
MKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDVNFVALDDGVSILNRLRLEGS
NTKADIVLGLDNNLMAEAKATGLLAQHQVETTAITLPNGWADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLK
VIYQDPRTSTPGQGLMLWMKSVYGDDVTGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAENDAQYA
AAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEGYRSLTVPEKALSFSAEEVAK
QRKAWIREWQSALTF

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=309, Percent_Identity=66.0194174757282, Blast_Score=449, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36849; Mature: 36849

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDV
CCCCHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCE
NFVALDDGVSILNRLRLEGSNTKADIVLGLDNNLMAEAKATGLLAQHQVETTAITLPNGW
EEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEEEECCCCC
ADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLKVIYQDPRTSTPGQGLMLWMK
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
SVYGDDVTGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAENDAQYA
HHCCCCCHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCEE
AAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEG
ECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
YRSLTVPEKALSFSAEEVAKQRKAWIREWQSALTF
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHTLNLIALASITSMMGISSSALAADKTLTIYTYDSFASDWGPGPTVEKAFEAQCGCDV
CCCCHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCE
NFVALDDGVSILNRLRLEGSNTKADIVLGLDNNLMAEAKATGLLAQHQVETTAITLPNGW
EEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCEEEEEEEECCCCC
ADDTFIPYDYGYFAFVYNKGKLANPPKSLKELVESRDDLKVIYQDPRTSTPGQGLMLWMK
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
SVYGDDVTGAWQQLAKKTVTVTKGWSEAYSMFLKGESDMVLSYTTSPAYHLIAENDAQYA
HHCCCCCHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCCEE
AAGFSEGHYMQVEVAAKVKSSQNSALADQFMSFILSDEFQSAMPTGNWMYPVTNVTLPEG
ECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
YRSLTVPEKALSFSAEEVAKQRKAWIREWQSALTF
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]