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The map label for this gene is rfbB1 [H]

Identifier: 37678484

GI number: 37678484

Start: 309344

End: 310405

Strand: Direct

Name: rfbB1 [H]

Synonym: VV0300

Alternate gene names: 37678484

Gene position: 309344-310405 (Clockwise)

Preceding gene: 37678483

Following gene: 37678485

Centisome position: 9.22

GC content: 46.42

Gene sequence:

>1062_bases
ATGAAAATATTAGTTACAGGTGGCGCTGGTTTTATCGGCTCTGCGGTCATCAGACACATCATCAATGACACGCGAGATAG
TGTCATTAACTTGGACAAACTCACCTATGCAGGTAACTTAGAGTCATTACACGAAGTTTCAAGCAGTGAGCGATATGTCT
TTGAACAAGTCGATATCTGTGATCGCACGGAGCTTGATCGTGTTTTTGCAGTGTATAAACCGGATGCGGTAATGCATCTG
GCTGCGGAATCGCATGTCGATCGTTCGATTGATGGTCCAGCCACATTTATTCAAACCAACATTGTTGGTACCTACAATTT
GTTGGAAGCGTCACGTGCCTATTGGTCGAATTTGGAAGAGAACGAGAAAGCACGTTTTCGTTTTCACCATATCTCGACCG
ACGAAGTATACGGAGATCTGGAAGGCACTGAAGATTTGTTCACTGAAAGTACATCTTACTCGCCGAGCAGCCCTTACTCC
GCCTCGAAAGCTTCCAGTGATCATCTTGTTCGTGCATGGCAGCGCACTTATGGATTACCCACGCTAGTAACCAACTGCTC
AAACAACTACGGACCTTACCATTTTCCAGAAAAACTGATCCCACTGATGATTCTCAATGCCCTAGAGGGTAAGCCGCTGC
CTGTTTACGGCGATGGTATGCAGATCCGCGATTGGCTATTTGTTGAAGATCACGCTCGTGCACTCTACAAAGCTGTGACC
GAAGGGGGCGTGGGTGAAACCTACAATATTGGTGGGCATAATGAAAAAGCCAACATTGAAGTGGTGAAAACCCTTTGTGC
ATTGTTAGAAGAGTACCGACCAAACAAGCCAGCAGGTATCGATGCTTACGAATCGCTGATTACCTACGTCAAAGATCGCC
CAGGACATGATGTTCGTTATGCAATTGATGCGTCAAAAATCGAACGAGAGTTGGGTTGGAAGCCAGAAGAGACGTTTGAA
TCAGGCATTCGCAAAACCGTTGAATGGTACCTCGCCAACCAAGCATGGTGGTCTCGCGTCTTAGATGGTTCTTATTCTCG
AGAACGTCTGGGCGTGAACTAA

Upstream 100 bases:

>100_bases
GGCTCAGTCGTACATCGCCAAATTATGAGTTATTCCTCGATACCATGGAGCATAAACGCTCATGGGCTGAGCAAGGTATT
GATACGTTAAAGGAACATCA

Downstream 100 bases:

>100_bases
CTTATCGCATTATTCAAATTTTTGGTCCTGTGCTCTCGTATTTTTCTTGAACCTAGGACCTAGAAAATGAGGACCTATTT
TAATGAAAGGTATAATTCTC

Product: dTDP-D-glucose 4;6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKILVTGGAGFIGSAVIRHIINDTRDSVINLDKLTYAGNLESLHEVSSSERYVFEQVDICDRTELDRVFAVYKPDAVMHL
AAESHVDRSIDGPATFIQTNIVGTYNLLEASRAYWSNLEENEKARFRFHHISTDEVYGDLEGTEDLFTESTSYSPSSPYS
ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGMQIRDWLFVEDHARALYKAVT
EGGVGETYNIGGHNEKANIEVVKTLCALLEEYRPNKPAGIDAYESLITYVKDRPGHDVRYAIDASKIERELGWKPEETFE
SGIRKTVEWYLANQAWWSRVLDGSYSRERLGVN

Sequences:

>Translated_353_residues
MKILVTGGAGFIGSAVIRHIINDTRDSVINLDKLTYAGNLESLHEVSSSERYVFEQVDICDRTELDRVFAVYKPDAVMHL
AAESHVDRSIDGPATFIQTNIVGTYNLLEASRAYWSNLEENEKARFRFHHISTDEVYGDLEGTEDLFTESTSYSPSSPYS
ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGMQIRDWLFVEDHARALYKAVT
EGGVGETYNIGGHNEKANIEVVKTLCALLEEYRPNKPAGIDAYESLITYVKDRPGHDVRYAIDASKIERELGWKPEETFE
SGIRKTVEWYLANQAWWSRVLDGSYSRERLGVN
>Mature_353_residues
MKILVTGGAGFIGSAVIRHIINDTRDSVINLDKLTYAGNLESLHEVSSSERYVFEQVDICDRTELDRVFAVYKPDAVMHL
AAESHVDRSIDGPATFIQTNIVGTYNLLEASRAYWSNLEENEKARFRFHHISTDEVYGDLEGTEDLFTESTSYSPSSPYS
ASKASSDHLVRAWQRTYGLPTLVTNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGMQIRDWLFVEDHARALYKAVT
EGGVGETYNIGGHNEKANIEVVKTLCALLEEYRPNKPAGIDAYESLITYVKDRPGHDVRYAIDASKIERELGWKPEETFE
SGIRKTVEWYLANQAWWSRVLDGSYSRERLGVN

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=36.6568914956012, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=24.5508982035928, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI56237023, Length=294, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI56118217, Length=294, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI189083684, Length=294, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=75.2840909090909, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=73.6543909348442, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=25.9887005649718, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788366, Length=299, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=37.7245508982036, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.8368580060423, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71982035, Length=371, Percent_Identity=26.1455525606469, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=373, Percent_Identity=26.0053619302949, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=341, Percent_Identity=24.9266862170088, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=284, Percent_Identity=25, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=284, Percent_Identity=25, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=284, Percent_Identity=25, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=284, Percent_Identity=25, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=284, Percent_Identity=25, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=381, Percent_Identity=27.5590551181102, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19923002, Length=365, Percent_Identity=28.2191780821918, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356223, Length=353, Percent_Identity=24.0793201133144, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39859; Mature: 39859

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVIRHIINDTRDSVINLDKLTYAGNLESLHEVSSSERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHEECCCHHHHHHHCCCCCEEHHHCCCC
DRTELDRVFAVYKPDAVMHLAAESHVDRSIDGPATFIQTNIVGTYNLLEASRAYWSNLEE
CHHHHHHEEEEECCCHHEEHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCCCCC
NEKARFRFHHISTDEVYGDLEGTEDLFTESTSYSPSSPYSASKASSDHLVRAWQRTYGLP
CCCCEEEEEECCHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
TLVTNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGMQIRDWLFVEDHARALYKAVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEEEHHHHHHHHHHHH
EGGVGETYNIGGHNEKANIEVVKTLCALLEEYRPNKPAGIDAYESLITYVKDRPGHDVRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEE
AIDASKIERELGWKPEETFESGIRKTVEWYLANQAWWSRVLDGSYSRERLGVN
EECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVIRHIINDTRDSVINLDKLTYAGNLESLHEVSSSERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHEECCCHHHHHHHCCCCCEEHHHCCCC
DRTELDRVFAVYKPDAVMHLAAESHVDRSIDGPATFIQTNIVGTYNLLEASRAYWSNLEE
CHHHHHHEEEEECCCHHEEHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCCCCC
NEKARFRFHHISTDEVYGDLEGTEDLFTESTSYSPSSPYSASKASSDHLVRAWQRTYGLP
CCCCEEEEEECCHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
TLVTNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGDGMQIRDWLFVEDHARALYKAVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEEEHHHHHHHHHHHH
EGGVGETYNIGGHNEKANIEVVKTLCALLEEYRPNKPAGIDAYESLITYVKDRPGHDVRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEE
AIDASKIERELGWKPEETFESGIRKTVEWYLANQAWWSRVLDGSYSRERLGVN
EECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]