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The map label for this gene is gloB1

Identifier: 37678337

GI number: 37678337

Start: 160328

End: 161071

Strand: Direct

Name: gloB1

Synonym: VV0153

Alternate gene names: 37678337

Gene position: 160328-161071 (Clockwise)

Preceding gene: 37678336

Following gene: 37678338

Centisome position: 4.78

GC content: 26.88

Gene sequence:

>744_bases
ATGTCTCTGAGTATAACCCATGAGAAATCATTGATTGATAATTACATCTGGATTTTATTTGATAAAAGCAAAGCTATTGT
TATTGATCCTGGTGAATCTGAAAAAATAATAAACTTCTTAGATAAGAACAATTTAACTTTAGAGTTTATTTTCTTAACTC
ATGGTCATTGCGATCATACAAAAGGAGTTTTTTCTTTAAAAGAGAAGTTTCCAAACGCTAGTCTGTTTGTACCAATTGGT
TTAGAATTAGGTTTGGGTGAAAGTATAATTAAAGAAGGTGATGAGTTAAATTTATTAAATTCTACATTTAATGTTTTAGA
GTTGCCAGGGCATACCGATAATCATATTGGAATAATGTATAAAGATAATTTGTTTTGTGGTGATGTTTTGTTTTCTGCAG
GCTGTGGAAGAGTGGGAAGTAATTATAAGGATATGTATCTTTCATTGAAAAAAATAAAAAGCATGGATGACAAAACAAAA
ATATATTTCTCTCATGAGTATACGCTAGATAATTTGAAATTTGCGCATTATATAGATCCAAAAAATAAAAACATAATAAA
TTATATAGAAGTTTTCAAGGAAAAACCAGACACAGTGTCAGCTCCCACTACATTATTATTGGAAAAGGAAATAAATCCAT
TTTTAAGGTTATCTGAAAATATTGATATTAAAAATAAAATAAACAATGAATTTGATGCTTTTGTTTACCTTAGAAAATTA
AAAGACAAATTTAATTCAATTTAA

Upstream 100 bases:

>100_bases
GCGGGTATAGTAATCAAAAAAATATTGTAGTATCAGATTACTACCACACTATAAAAAAATCGTTATAAAATAAAATTTTG
GTTAAAAGAGTATATATCAA

Downstream 100 bases:

>100_bases
AAATATAAATAAAGGATAAAAATTATGAAATTAAAAACTATTATATTAATAATTGGTTTTTTATCATCTCCTAGTATTGC
TTTTGCTAATGAAAATAAAG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II 1; Glx II 1

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSLSITHEKSLIDNYIWILFDKSKAIVIDPGESEKIINFLDKNNLTLEFIFLTHGHCDHTKGVFSLKEKFPNASLFVPIG
LELGLGESIIKEGDELNLLNSTFNVLELPGHTDNHIGIMYKDNLFCGDVLFSAGCGRVGSNYKDMYLSLKKIKSMDDKTK
IYFSHEYTLDNLKFAHYIDPKNKNIINYIEVFKEKPDTVSAPTTLLLEKEINPFLRLSENIDIKNKINNEFDAFVYLRKL
KDKFNSI

Sequences:

>Translated_247_residues
MSLSITHEKSLIDNYIWILFDKSKAIVIDPGESEKIINFLDKNNLTLEFIFLTHGHCDHTKGVFSLKEKFPNASLFVPIG
LELGLGESIIKEGDELNLLNSTFNVLELPGHTDNHIGIMYKDNLFCGDVLFSAGCGRVGSNYKDMYLSLKKIKSMDDKTK
IYFSHEYTLDNLKFAHYIDPKNKNIINYIEVFKEKPDTVSAPTTLLLEKEINPFLRLSENIDIKNKINNEFDAFVYLRKL
KDKFNSI
>Mature_246_residues
SLSITHEKSLIDNYIWILFDKSKAIVIDPGESEKIINFLDKNNLTLEFIFLTHGHCDHTKGVFSLKEKFPNASLFVPIGL
ELGLGESIIKEGDELNLLNSTFNVLELPGHTDNHIGIMYKDNLFCGDVLFSAGCGRVGSNYKDMYLSLKKIKSMDDKTKI
YFSHEYTLDNLKFAHYIDPKNKNIINYIEVFKEKPDTVSAPTTLLLEKEINPFLRLSENIDIKNKINNEFDAFVYLRKLK
DKFNSI

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI94538322, Length=262, Percent_Identity=29.0076335877863, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI94538320, Length=262, Percent_Identity=29.0076335877863, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI116642887, Length=220, Percent_Identity=29.5454545454545, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI21703352, Length=220, Percent_Identity=29.5454545454545, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=27.7227722772277, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1786406, Length=242, Percent_Identity=40.9090909090909, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17536925, Length=258, Percent_Identity=30.2325581395349, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320478, Length=259, Percent_Identity=31.2741312741313, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356335, Length=257, Percent_Identity=32.295719844358, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24667703, Length=257, Percent_Identity=32.295719844358, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24667711, Length=257, Percent_Identity=31.9066147859922, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO21_VIBVY (Q7MQ55)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_932946.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q7MQ55
- SMR:   Q7MQ55
- STRING:   Q7MQ55
- GeneID:   2622912
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV0153
- NMPDR:   fig|196600.1.peg.222
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   YAGHDYV
- BioCyc:   VVUL196600:VV0153-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28352; Mature: 28221

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSITHEKSLIDNYIWILFDKSKAIVIDPGESEKIINFLDKNNLTLEFIFLTHGHCDHT
CCEECCCHHHHHCCEEEEEEECCCEEEECCCCCHHHHHHHCCCCCEEEEEEEECCCCCCC
KGVFSLKEKFPNASLFVPIGLELGLGESIIKEGDELNLLNSTFNVLELPGHTDNHIGIMY
CCHHHHHHHCCCCEEEEEECEEECCCHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEEE
KDNLFCGDVLFSAGCGRVGSNYKDMYLSLKKIKSMDDKTKIYFSHEYTLDNLKFAHYIDP
ECCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEECC
KNKNIINYIEVFKEKPDTVSAPTTLLLEKEINPFLRLSENIDIKNKINNEFDAFVYLRKL
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHCCCHHHHHHHHHHH
KDKFNSI
HHHHCCC
>Mature Secondary Structure 
SLSITHEKSLIDNYIWILFDKSKAIVIDPGESEKIINFLDKNNLTLEFIFLTHGHCDHT
CEECCCHHHHHCCEEEEEEECCCEEEECCCCCHHHHHHHCCCCCEEEEEEEECCCCCCC
KGVFSLKEKFPNASLFVPIGLELGLGESIIKEGDELNLLNSTFNVLELPGHTDNHIGIMY
CCHHHHHHHCCCCEEEEEECEEECCCHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEEE
KDNLFCGDVLFSAGCGRVGSNYKDMYLSLKKIKSMDDKTKIYFSHEYTLDNLKFAHYIDP
ECCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEEEECC
KNKNIINYIEVFKEKPDTVSAPTTLLLEKEINPFLRLSENIDIKNKINNEFDAFVYLRKL
CCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHCCCHHHHHHHHHHH
KDKFNSI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA