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The map label for this gene is lexA

Identifier: 37678307

GI number: 37678307

Start: 130780

End: 131403

Strand: Direct

Name: lexA

Synonym: VV0123

Alternate gene names: 37678307

Gene position: 130780-131403 (Clockwise)

Preceding gene: 37678305

Following gene: 37678308

Centisome position: 3.9

GC content: 49.68

Gene sequence:

>624_bases
ATGAAGCCGTTAACGCCACGCCAACAACAAGTTTTCGATCTGATTAAAAGCAAGATCGAAGTGACTGGAATGCCACCCAC
TCGTGCGGAAATTGCGCGTGAGCTGGGTTTTCGCTCTGCCAATGCGGCAGAAGAACATTTAAAAGCGCTTGCTCGTAAGC
AAGTGATCGAAATTGTCCCAGGTGCCTCTCGTGGTATCCGTATTCTGCTTGAAGAGGAAGCGGCGAATGATGAGCCAGGT
TTACCTCTGATTGGCCGTGTTGCGGCAGGGGAGCCGATCTTGGCGCAAGAGCATGTGGAAATGCACTACCAAGTGGATCC
GAGCATGTTCCGACCTCAAGCCGATTTTCTTTTGCGCGTTCATGGCGAAAGTATGAAAGACATCGGCATTATGGATGGTG
ACTTGTTGGCTGTGCACAAAACACAAGACGTGCGCAATGGGCAAGTTGTGGTCGCGCGCGTTGAAGATGATGTAACGGTA
AAACGTTTGGAACGTAAAGGTTCAACCGTGCTGCTCCATGCAGAAAATGAAGAGTTTGCACCTATCGAAGTGGATCTCAC
TAGCCAGCAGCTGACTATTGAAGGCATTGCCGTGGGTATTATCCGCAATACGGATTGGATGTAA

Upstream 100 bases:

>100_bases
GCCAAAAAGAGAGAAGAAATTAAAAAACAATCAATTGGGTTTCCATTTGTACAATTACTGGATATACTCACAGTTAACTG
TATAAAAAGACAGGTGACCT

Downstream 100 bases:

>100_bases
TACGCCCAAATGTGCAAAGCAATGCTCAATAAGCTCCGTTTCTGCGGAGCTTATTTGTTTTGTCTCGACCCCATTTGACG
ATCGTTTCTCAATAAGGCGT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKPLTPRQQQVFDLIKSKIEVTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIVPGASRGIRILLEEEAANDEPG
LPLIGRVAAGEPILAQEHVEMHYQVDPSMFRPQADFLLRVHGESMKDIGIMDGDLLAVHKTQDVRNGQVVVARVEDDVTV
KRLERKGSTVLLHAENEEFAPIEVDLTSQQLTIEGIAVGIIRNTDWM

Sequences:

>Translated_207_residues
MKPLTPRQQQVFDLIKSKIEVTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIVPGASRGIRILLEEEAANDEPG
LPLIGRVAAGEPILAQEHVEMHYQVDPSMFRPQADFLLRVHGESMKDIGIMDGDLLAVHKTQDVRNGQVVVARVEDDVTV
KRLERKGSTVLLHAENEEFAPIEVDLTSQQLTIEGIAVGIIRNTDWM
>Mature_207_residues
MKPLTPRQQQVFDLIKSKIEVTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIVPGASRGIRILLEEEAANDEPG
LPLIGRVAAGEPILAQEHVEMHYQVDPSMFRPQADFLLRVHGESMKDIGIMDGDLLAVHKTQDVRNGQVVVARVEDDVTV
KRLERKGSTVLLHAENEEFAPIEVDLTSQQLTIEGIAVGIIRNTDWM

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=207, Percent_Identity=74.3961352657005, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_VIBVU (Q8DD47)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760110.1
- ProteinModelPortal:   Q8DD47
- SMR:   Q8DD47
- GeneID:   1178092
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1166
- HOGENOM:   HBG679610
- OMA:   VGVWRGY
- ProtClustDB:   PRK00215
- BioCyc:   VVUL216895:VV1_1166-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22995; Mature: 22995

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: ACT_SITE 124-124 ACT_SITE 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLTPRQQQVFDLIKSKIEVTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIVP
CCCCCCHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GASRGIRILLEEEAANDEPGLPLIGRVAAGEPILAQEHVEMHYQVDPSMFRPQADFLLRV
CCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEECCCEEEEEEECHHHHCCCCCEEEEE
HGESMKDIGIMDGDLLAVHKTQDVRNGQVVVARVEDDVTVKRLERKGSTVLLHAENEEFA
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCC
PIEVDLTSQQLTIEGIAVGIIRNTDWM
EEEEEECCCEEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MKPLTPRQQQVFDLIKSKIEVTGMPPTRAEIARELGFRSANAAEEHLKALARKQVIEIVP
CCCCCCHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GASRGIRILLEEEAANDEPGLPLIGRVAAGEPILAQEHVEMHYQVDPSMFRPQADFLLRV
CCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCEECCCEEEEEEECHHHHCCCCCEEEEE
HGESMKDIGIMDGDLLAVHKTQDVRNGQVVVARVEDDVTVKRLERKGSTVLLHAENEEFA
CCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCC
PIEVDLTSQQLTIEGIAVGIIRNTDWM
EEEEEECCCEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA