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The map label for this gene is rhtB

Identifier: 37678281

GI number: 37678281

Start: 109141

End: 109764

Strand: Direct

Name: rhtB

Synonym: VV0097

Alternate gene names: 37678281

Gene position: 109141-109764 (Clockwise)

Preceding gene: 37678278

Following gene: 37678282

Centisome position: 3.25

GC content: 50.16

Gene sequence:

>624_bases
ATGCAAATGGATACCCATGTTTGGTTGGCGTATGTTGTGACCGCAATCGTTTTCAGTTTGGCACCGGGCTCTGGCACGGT
GAACTCAATCAGCAATGGCCTCAGTTATGGCACGCGAAAATCGCTGGCTTCCATTGTGGGGCTGCAAATTGGTTTGGCGG
TCCACATTGTCCTGGTTGGGGCGGGCATTGGTGCTTTGGTGGCACAATCGGCGACGGCATTCACGGTGATTAAGTGGGTG
GGTGCGGTGTATTTGGTTTGGCTGGGCATTCAAAAATGGCGTGATACGTCAAGCCTCGCGACGGCCTCGCAACAACATGC
CATTTCCAGCACTGCGCTATTACGCAAAGCGGTGCTGATCAATCTGACCAATCCGAAATCGATCGTCTTTCTAGTGGCGC
TGTTTCCGCAATTTATCGATCCAAGCCAAGATCAAGTGACCCAGTTAGCGGTGTTAGGCATCACGACCGTGGTGATTGAT
GCGTTTGTCATGCTCGGCTACACCACCTTGGCATCTCAACTGGGGCGTTTTATCCGTTCAGAGAAAGTGATGGGCAAAAT
AAACAAAGTATTTGGTTCAATGTTTATGGGTTGTGGGGCATTATTAGCAGCTGCAAAATCATAA

Upstream 100 bases:

>100_bases
AAGCCTCTTGCGTATAAGAGAGAGGTGAGCTGTTATTAACCATTAAATAAATACCTGATCATGCGTTTCTGTTGTCAATG
ATCAGAGAATTATCGGGGAA

Downstream 100 bases:

>100_bases
CTAGCAGTGATAAATTCACTGGTTAGTCACTGAGCATAAGAATAAAGCACTAAATGAATACCACATACGTCGCACGGCAG
CCGATCTTTAATCGGAAGAA

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQMDTHVWLAYVVTAIVFSLAPGSGTVNSISNGLSYGTRKSLASIVGLQIGLAVHIVLVGAGIGALVAQSATAFTVIKWV
GAVYLVWLGIQKWRDTSSLATASQQHAISSTALLRKAVLINLTNPKSIVFLVALFPQFIDPSQDQVTQLAVLGITTVVID
AFVMLGYTTLASQLGRFIRSEKVMGKINKVFGSMFMGCGALLAAAKS

Sequences:

>Translated_207_residues
MQMDTHVWLAYVVTAIVFSLAPGSGTVNSISNGLSYGTRKSLASIVGLQIGLAVHIVLVGAGIGALVAQSATAFTVIKWV
GAVYLVWLGIQKWRDTSSLATASQQHAISSTALLRKAVLINLTNPKSIVFLVALFPQFIDPSQDQVTQLAVLGITTVVID
AFVMLGYTTLASQLGRFIRSEKVMGKINKVFGSMFMGCGALLAAAKS
>Mature_207_residues
MQMDTHVWLAYVVTAIVFSLAPGSGTVNSISNGLSYGTRKSLASIVGLQIGLAVHIVLVGAGIGALVAQSATAFTVIKWV
GAVYLVWLGIQKWRDTSSLATASQQHAISSTALLRKAVLINLTNPKSIVFLVALFPQFIDPSQDQVTQLAVLGITTVVID
AFVMLGYTTLASQLGRFIRSEKVMGKINKVFGSMFMGCGALLAAAKS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=210, Percent_Identity=53.8095238095238, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788099, Length=205, Percent_Identity=29.7560975609756, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21859; Mature: 21859

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMDTHVWLAYVVTAIVFSLAPGSGTVNSISNGLSYGTRKSLASIVGLQIGLAVHIVLVG
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AGIGALVAQSATAFTVIKWVGAVYLVWLGIQKWRDTSSLATASQQHAISSTALLRKAVLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLTNPKSIVFLVALFPQFIDPSQDQVTQLAVLGITTVVIDAFVMLGYTTLASQLGRFIRS
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVMGKINKVFGSMFMGCGALLAAAKS
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQMDTHVWLAYVVTAIVFSLAPGSGTVNSISNGLSYGTRKSLASIVGLQIGLAVHIVLVG
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AGIGALVAQSATAFTVIKWVGAVYLVWLGIQKWRDTSSLATASQQHAISSTALLRKAVLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLTNPKSIVFLVALFPQFIDPSQDQVTQLAVLGITTVVIDAFVMLGYTTLASQLGRFIRS
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKVMGKINKVFGSMFMGCGALLAAAKS
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]