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The map label for this gene is dapF

Identifier: 37678271

GI number: 37678271

Start: 98437

End: 99267

Strand: Direct

Name: dapF

Synonym: VV0087

Alternate gene names: 37678271

Gene position: 98437-99267 (Clockwise)

Preceding gene: 37678270

Following gene: 37678272

Centisome position: 2.93

GC content: 50.66

Gene sequence:

>831_bases
ATGCATTTCCATTTTTCTAAAATGCACGGTTTGGGTAACGACTTTATGGTGGTCGACTGCATTACTCAAAACGTTTACTT
CTCTCAAGAGCTGATCCGCCGTTTGGCGGATCGCCATACTGGCGTTGGCTTTGACCAATTGCTGGTGGTGGAAGCACCTT
ACGATCCTGAGACGGATTTCCACTATCGTATTTTCAATGCCGATGGCAGTGAAGTGGAACAGTGTGGTAATGGCGCGCGC
TGTTTCGCTCGTTTTGTTCGCATGAAAGGGCTGACTAATAAGTACAGCATCAGCGTCAGCACCAAGAAAGGCAAAATGAT
TCTTGATGTGGAAGAGGACGATCAAATCACCGTCAACATGGGTGTGCCGGAGTTTGAACCGAACAAAATCCCTTTTCGCG
CCAAGCAAAAAGAGAAAACCTACATCATGCGTGTTGGCGAAAAAACGCTGTTTTGTGGTGCGGTGAGCATGGGCAACCCA
CATGTTGTGACTGTGGTGGATGACGTCGATAGCGCAGAAGTGGAGACGTTAGGACCATTGCTTGAATCGCATGAGCGTTT
CCCAGAGCGCGTCAATGCGGGATTTATGCAAGTGGTCAACCGTGAACAGATCCGTCTGCGCGTGTATGAGCGTGGTGCGG
GTGAAACGCAGGCTTGTGGCAGCGGTGCGTGTGGCGCGGTGGCGGTCGGCATCTTACAAGGCTTGTTGGATGAAAATGTC
ACCGTGAGTTTGCCCGGTGGCGATTTGCACATTCATTGGCAAGGTCCGGGTAAGCCACTTTACATGACTGGCCCTGCCAC
TCATGTGTTTGATGGCCAGCTCTCTTGTTAA

Upstream 100 bases:

>100_bases
GTGATGGTGGATGGCAAACAGCATTATCTGGTACGCCAGCGCGAAGAATTGCGTAGCTTGTGGGAACTAGAACACATTCT
TCCGGAGTAACGAGCTTTTT

Downstream 100 bases:

>100_bases
GCGCTATCCGCTCGTTGAGTTTGTATCGTTAGAGGAAACGTTTTGTCTTACCCTGAAGCCGATGCTCTGACCGCCGAAGT
GGTGGCGGAGTATCTTCAAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHFHFSKMHGLGNDFMVVDCITQNVYFSQELIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSISVSTKKGKMILDVEEDDQITVNMGVPEFEPNKIPFRAKQKEKTYIMRVGEKTLFCGAVSMGNP
HVVTVVDDVDSAEVETLGPLLESHERFPERVNAGFMQVVNREQIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENV
TVSLPGGDLHIHWQGPGKPLYMTGPATHVFDGQLSC

Sequences:

>Translated_276_residues
MHFHFSKMHGLGNDFMVVDCITQNVYFSQELIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSISVSTKKGKMILDVEEDDQITVNMGVPEFEPNKIPFRAKQKEKTYIMRVGEKTLFCGAVSMGNP
HVVTVVDDVDSAEVETLGPLLESHERFPERVNAGFMQVVNREQIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENV
TVSLPGGDLHIHWQGPGKPLYMTGPATHVFDGQLSC
>Mature_276_residues
MHFHFSKMHGLGNDFMVVDCITQNVYFSQELIRRLADRHTGVGFDQLLVVEAPYDPETDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYSISVSTKKGKMILDVEEDDQITVNMGVPEFEPNKIPFRAKQKEKTYIMRVGEKTLFCGAVSMGNP
HVVTVVDDVDSAEVETLGPLLESHERFPERVNAGFMQVVNREQIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENV
TVSLPGGDLHIHWQGPGKPLYMTGPATHVFDGQLSC

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=270, Percent_Identity=77.4074074074074, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30616; Mature: 30616

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNVYFSQELIRRLADRHTGVGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYSISVSTKKGKMILDVEEDDQITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
GVPEFEPNKIPFRAKQKEKTYIMRVGEKTLFCGAVSMGNPHVVTVVDDVDSAEVETLGPL
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHH
LESHERFPERVNAGFMQVVNREQIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENV
HHHHHHHHHHHCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
TVSLPGGDLHIHWQGPGKPLYMTGPATHVFDGQLSC
EEEECCCEEEEEEECCCCCEEEECCCCEEECCCCCC
>Mature Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNVYFSQELIRRLADRHTGVGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYSISVSTKKGKMILDVEEDDQITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
GVPEFEPNKIPFRAKQKEKTYIMRVGEKTLFCGAVSMGNPHVVTVVDDVDSAEVETLGPL
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHH
LESHERFPERVNAGFMQVVNREQIRLRVYERGAGETQACGSGACGAVAVGILQGLLDENV
HHHHHHHHHHHCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
TVSLPGGDLHIHWQGPGKPLYMTGPATHVFDGQLSC
EEEECCCEEEEEEECCCCCEEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA