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The map label for this gene is rep [H]

Identifier: 37678224

GI number: 37678224

Start: 46389

End: 48407

Strand: Direct

Name: rep [H]

Synonym: VV0040

Alternate gene names: 37678224

Gene position: 46389-48407 (Clockwise)

Preceding gene: 37678219

Following gene: 37678227

Centisome position: 1.38

GC content: 49.03

Gene sequence:

>2019_bases
ATGAAACTGAATCCAAGACAAGACGAAGCCGTGAAATACGTATCAGGTCCCTGCCTCGTGCTTGCGGGTGCGGGTTCAGG
CAAAACACGTGTGATCACCAATAAGATTGCCTATTTGGTGCAACAGTGTGGCTACAAAGCGCGTAATATTGCGGCGGTCA
CCTTCACCAATAAAGCCGCGCGTGAGATGAAAGAACGTGTGGGGCAAACACTGGGGAAGGGGGAGTCAAAAGGCCTGATG
GTGTCAACCTTCCACACACTTGGTTTGAATATCATTAAGCGAGAGTATCGCCATCTGGGTTTGAAGTCGGGCTTTTCCTT
GTTTGACGATCAAGATCAGCTTGCACTGCTTAAAGAGCTGACTGAAAAACAGCTGGATGGCGATAAAGATTTGCTGCGTC
AGTTAATGAGCACCATCTCAAATTGGAAAAACGACATGCTCTCACCAGAGCAGGCCGTGGCACGTGCGCAAGGCGAACAG
CAGCAGTTGTTTGCCTTCTGTTTTGAGATGTATCAGAAGCAGATGAAAGCGTACAACGCCCTCGACTTTGATGATCTTAT
CTTGCTGCCAGTGTTACTACTTCGTGGGCATGATGAGGTGCGTCACTACTGGCAAAATCGTATCCGTTACCTTCTGGTCG
ATGAGTATCAAGATACCAACACCAGTCAATATGAACTGGTGAAACTGCTGGTGGGGGAGCGCGGACGCTTAACCGTGGTT
GGGGATGATGATCAATCGATTTACTCTTGGCGTGGCGCGAAACCGCAAAACTTAGTGTTGCTGGGGCAAGATTATCCCAA
CTTACGCCTGATTAAATTGGAGCAAAACTACCGCTCGACCAGCCGTATTTTGCGCGCGGCGAATATCTTGATTGCCAATA
ACCCACACGTGTATGAAAAGTCGCTCTTCTCGGAAATTCCCGATGGTGAAAAACTCAAAGTGCTGCTGGCCAATAACGAC
GAGCATGAGGCGGAGCGCGTCACGGGGGAAATCATTGCCCACAAGTTTCTCAATCGCACCGACTATCGTGACTACGCCAT
CCTCTATCGTGGTAACCATCAATCGCGTCTCATCGAAAAGTCGCTGATGCAAAACCGCGTTCCTTATCGACTGTCTGGTG
GTACGTCTTTCTTTGCGCGCGCGGAGATCAAAGACATCATGGCGTATTTGCGAGTGTTGGTGAATCCTGATGATGACAAC
GCATTTTTGCGCATCGTCAACACACCCAAGCGTGAAATTGGCCCTGTGACGCTGGAGAAGTTAGGCAGCTATGCCAACAT
GCGTGGCAAAAGTTTATTTGAAGCCAGCTTTGAACTTGGCCTTGAGCAGCACCTGACCGGACGTGGTCTCGATAATTTGC
GGCGCTTTACTCATTGGCTGGTGGCGATTGCCGATCAAGCGGAGCGAGGGGATACCGTGGAAGCGGTGCGCTCGTTGGTG
CGAGACATTCATTATGAGGATTGGTTGTACGAAACATCTGCCAGCCCGAAAGCCGCAGAAATGCGCATGAAAAACGTCTC
TGATCTCTACTCTTGGATTGTCGCTGATTTAGAAGGTGACAATAACGATCAAGAAGAGAAAACGTTGAAAGAAGTGGTTC
AACGTTTGACACTGCGTGACATGATGGAACGTGGTGAAGAAGATGAAGATAGTGATGCCGTACAACTGATGACACTGCAT
GCGTCTAAAGGTCTGGAATTTCCATATGTGTACCTGATCGGGACTGAAGAAGGGATATTGCCGCACCAAACCAGTATCGA
TGAAGATAACGTCGAAGAAGAACGTCGCTTAATGTACGTCGGCATTACGCGTGCGCAGCGAGAACTGACTTTCGTGATGT
GTAAAGAGCGTCGTCAATATGGGGAGTTGATCAAACCCAGCCAAAGTCGCTTCCTAGACGAGTTGCCTTTCGATGATGTG
GAATGGGAACAAACCAAAAAGCCAGTGACACAGGAAGAAAGAATGGCCAAAGGCCAAGCGCACATTGCCAACTTACGTGC
CATGTTCAACAAGAAGTAG

Upstream 100 bases:

>100_bases
ATGTGTGCTATTGAGTAAAAATCTTGTCAATGAGGCAATGGCAATCTCATCACTCGACGACTATTATATGGGCCTCGATT
TTTGACCACAGATAAGCGCT

Downstream 100 bases:

>100_bases
CGTTAGTCAAATAAGAAAAGGGCTTGGAAAACCAAGCCCTTTTGCTTTAGGAAACTTTGTTTTATAGACCTTTGATCATG
TGATCAATCGCGGCAATGAT

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLM
VSTFHTLGLNIIKREYRHLGLKSGFSLFDDQDQLALLKELTEKQLDGDKDLLRQLMSTISNWKNDMLSPEQAVARAQGEQ
QQLFAFCFEMYQKQMKAYNALDFDDLILLPVLLLRGHDEVRHYWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGQDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLLANND
EHEAERVTGEIIAHKFLNRTDYRDYAILYRGNHQSRLIEKSLMQNRVPYRLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPVTLEKLGSYANMRGKSLFEASFELGLEQHLTGRGLDNLRRFTHWLVAIADQAERGDTVEAVRSLV
RDIHYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNNDQEEKTLKEVVQRLTLRDMMERGEEDEDSDAVQLMTLH
ASKGLEFPYVYLIGTEEGILPHQTSIDEDNVEEERRLMYVGITRAQRELTFVMCKERRQYGELIKPSQSRFLDELPFDDV
EWEQTKKPVTQEERMAKGQAHIANLRAMFNKK

Sequences:

>Translated_672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLM
VSTFHTLGLNIIKREYRHLGLKSGFSLFDDQDQLALLKELTEKQLDGDKDLLRQLMSTISNWKNDMLSPEQAVARAQGEQ
QQLFAFCFEMYQKQMKAYNALDFDDLILLPVLLLRGHDEVRHYWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGQDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLLANND
EHEAERVTGEIIAHKFLNRTDYRDYAILYRGNHQSRLIEKSLMQNRVPYRLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPVTLEKLGSYANMRGKSLFEASFELGLEQHLTGRGLDNLRRFTHWLVAIADQAERGDTVEAVRSLV
RDIHYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNNDQEEKTLKEVVQRLTLRDMMERGEEDEDSDAVQLMTLH
ASKGLEFPYVYLIGTEEGILPHQTSIDEDNVEEERRLMYVGITRAQRELTFVMCKERRQYGELIKPSQSRFLDELPFDDV
EWEQTKKPVTQEERMAKGQAHIANLRAMFNKK
>Mature_672_residues
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAAREMKERVGQTLGKGESKGLM
VSTFHTLGLNIIKREYRHLGLKSGFSLFDDQDQLALLKELTEKQLDGDKDLLRQLMSTISNWKNDMLSPEQAVARAQGEQ
QQLFAFCFEMYQKQMKAYNALDFDDLILLPVLLLRGHDEVRHYWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
GDDDQSIYSWRGAKPQNLVLLGQDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEKSLFSEIPDGEKLKVLLANND
EHEAERVTGEIIAHKFLNRTDYRDYAILYRGNHQSRLIEKSLMQNRVPYRLSGGTSFFARAEIKDIMAYLRVLVNPDDDN
AFLRIVNTPKREIGPVTLEKLGSYANMRGKSLFEASFELGLEQHLTGRGLDNLRRFTHWLVAIADQAERGDTVEAVRSLV
RDIHYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNNDQEEKTLKEVVQRLTLRDMMERGEEDEDSDAVQLMTLH
ASKGLEFPYVYLIGTEEGILPHQTSIDEDNVEEERRLMYVGITRAQRELTFVMCKERRQYGELIKPSQSRFLDELPFDDV
EWEQTKKPVTQEERMAKGQAHIANLRAMFNKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=71.7261904761905, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=40, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787196, Length=362, Percent_Identity=27.9005524861878, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=708, Percent_Identity=27.5423728813559, Blast_Score=204, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77428; Mature: 77428

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEECCHHH
REMKERVGQTLGKGESKGLMVSTFHTLGLNIIKREYRHLGLKSGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHH
TEKQLDGDKDLLRQLMSTISNWKNDMLSPEQAVARAQGEQQQLFAFCFEMYQKQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPVLLLRGHDEVRHYWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGQDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEK
ECCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCCHHHH
SLFSEIPDGEKLKVLLANNDEHEAERVTGEIIAHKFLNRTDYRDYAILYRGNHQSRLIEK
HHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLMQNRVPYRLSGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPKREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGSYANMRGKSLFEASFELGLEQHLTGRGLDNLRRFTHWLVAIADQAERGDTVEAVRSLV
HCCHHHCCCHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDIHYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNNDQEEKTLKEVVQRLTLRD
HHCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEEDEDSDAVQLMTLHASKGLEFPYVYLIGTEEGILPHQTSIDEDNVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEE
GITRAQRELTFVMCKERRQYGELIKPSQSRFLDELPFDDVEWEQTKKPVTQEERMAKGQA
EEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
HIANLRAMFNKK
HHHHHHHHHCCC
>Mature Secondary Structure
MKLNPRQDEAVKYVSGPCLVLAGAGSGKTRVITNKIAYLVQQCGYKARNIAAVTFTNKAA
CCCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHCEEEEEECCHHH
REMKERVGQTLGKGESKGLMVSTFHTLGLNIIKREYRHLGLKSGFSLFDDQDQLALLKEL
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHH
TEKQLDGDKDLLRQLMSTISNWKNDMLSPEQAVARAQGEQQQLFAFCFEMYQKQMKAYNA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPVLLLRGHDEVRHYWQNRIRYLLVDEYQDTNTSQYELVKLLVGERGRLTVV
CCHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYSWRGAKPQNLVLLGQDYPNLRLIKLEQNYRSTSRILRAANILIANNPHVYEK
ECCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHEEEECCCCHHHH
SLFSEIPDGEKLKVLLANNDEHEAERVTGEIIAHKFLNRTDYRDYAILYRGNHQSRLIEK
HHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
SLMQNRVPYRLSGGTSFFARAEIKDIMAYLRVLVNPDDDNAFLRIVNTPKREIGPVTLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCCHHHHH
LGSYANMRGKSLFEASFELGLEQHLTGRGLDNLRRFTHWLVAIADQAERGDTVEAVRSLV
HCCHHHCCCHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDIHYEDWLYETSASPKAAEMRMKNVSDLYSWIVADLEGDNNDQEEKTLKEVVQRLTLRD
HHCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
MMERGEEDEDSDAVQLMTLHASKGLEFPYVYLIGTEEGILPHQTSIDEDNVEEERRLMYV
HHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEE
GITRAQRELTFVMCKERRQYGELIKPSQSRFLDELPFDDVEWEQTKKPVTQEERMAKGQA
EEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
HIANLRAMFNKK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]