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The map label for this gene is gatA2 [H]

Identifier: 37523623

GI number: 37523623

Start: 4247615

End: 4249174

Strand: Direct

Name: gatA2 [H]

Synonym: glr4054

Alternate gene names: 37523623

Gene position: 4247615-4249174 (Clockwise)

Preceding gene: 37523621

Following gene: 37523626

Centisome position: 91.17

GC content: 65.77

Gene sequence:

>1560_bases
ATGGCGGCCGCGGGCGGGCTGTCGATTTTACTGGGTACCGGCCGGGCGTTCGCCGCCACCTGCCCGCCTGCCGGTCCGCC
GTTGGCGGAGACGACGGTGGCCGCTCTGCAGAAGGCCCTGGCCTCCGGGGCGCTCACCTCGCGCCGGATTGTCCAGGGCT
ACCTCGATCGGATCGCCTGCTACGACAAGCAGGGTCCAAAGATCAACGCCGTGCTCGAAATCAACCCGGACGCGCTCGCC
ATCGCCGACGCGCTCGACGCCGAGCGGCGGGCGGGCAAGGTGCGCGGACCTCTACACGGCATTCCGATACTGCTCAAGGG
CAACATCGCCACGGACGACCGGATGCTCACCACCGCCGGTTCGGTGGCGCTCGTCGATTCGCTGCCCCAAAAGGACGCCT
TTATCGCCACCCGTCTGCGCGAGGCGGGGACGGTGCTGTTGGGAAAGGCCAACCTGACTGAGTTTGCCAACTTCATGTCC
TACTACATGCCCTCGGGCTACAGCTCCCAGGGCGGCCAGACCCTCAATCCGTACTTTCCGGCCCTCGAAGACAACGGTGT
GCCCACGGTCACCCCCTGCGGCTCCAGCGCCGGTTCGGGGGCGGCGACGGCGGCGAACCTGACGGCCATTTCGATCGGCA
CCGAGACCTCCGGCTCGATTCTCTGTCCCTCCAGCTTCAATTCGCTGGTGGGGATCAAGCCGACCGTGGGCCTGGTGAGC
CGCACCGGCATCATCCCGATCTCGGCAAGCCAGGATGTGGCAGGACCGATGACCCGCACGGTGGCCGATGCGGCGGTGCT
GCTTGGTGCCATTGCCGGGTACGACCCGGCCGACCCGGTGACCGCGAGCAGCGTGGGCCAGATCCCGGCCGATTACCGAA
CTTTTCTTAAACTGGATGGTCTGGTGGGGGTACGCATTGGCCTGCCCCCGGAATATCTTGATTTTTTGGGTCCGGAGACC
CGTCCTGCCTTCGATCAGGCCCTCGCCGTGCTGCGCGCCCAGGGAGCGGTGATCGTCGATGCGCCGATCGCCACCACCGA
CGCGCTGTTTGCCTCCCCGTCGGTGATCACCGTCCTGACCTACGAGTTCAAGCGCGATCTCAATGCCTATCTGGCCAAGG
TCAAGCCCGGTACCACGATCGACACGCTTTCCGAAGTGATCGACTTCAATTTCCGCAAACGCGAAGTGGCCCTCAAGTAC
GGCCAGGGACTGCTGGTCGACAGCCAACAGCGCCGATTGCAGGACGGCACCCCGATCACCGCCCAGGGCTATCGCGACGC
CCTTGCCGAGAAGAGGCTGCTCGCCAAAACCGAGGGCATCGATGCCACCATCGCGAAGTATGACCTCGACGCGCTGTTGT
TTCCGACTTATTACGGCTCGTTCGTCGGCGCGGCTGCCGGGTATCCGTCGGTGATCGTCCCGGCGGGCTACGCCACAGGC
GGGCTGCCGATCGGCATCACCTTCCTGGGCAAGGCCTTCAGCGAGCCGCAGCTGATCCAGTACGCCTACGCCTACGAGCA
GGCTTCCCTCGCCCGCCGTCCGCCGGAAGCTACGCCCTGA

Upstream 100 bases:

>100_bases
TCCGCGATTGCTGTATTCGGTCTACCGAACGTGGTATGGATATCCAGCAATCCAAGCTGCTGGGGGTTCTATGGTGTTGA
GGAAACCAGGCTTCGCGACG

Downstream 100 bases:

>100_bases
CGGCGGGGCCTGAGGCGATTAGCCGCAGGCAAAAGAGCATATAGAGCGTCCAGCCCAGGGGACCCACCAGCCGCAAAGCC
GGGCCGATCCCGGCGATGGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAAAGGLSILLGTGRAFAATCPPAGPPLAETTVAALQKALASGALTSRRIVQGYLDRIACYDKQGPKINAVLEINPDALA
IADALDAERRAGKVRGPLHGIPILLKGNIATDDRMLTTAGSVALVDSLPQKDAFIATRLREAGTVLLGKANLTEFANFMS
YYMPSGYSSQGGQTLNPYFPALEDNGVPTVTPCGSSAGSGAATAANLTAISIGTETSGSILCPSSFNSLVGIKPTVGLVS
RTGIIPISASQDVAGPMTRTVADAAVLLGAIAGYDPADPVTASSVGQIPADYRTFLKLDGLVGVRIGLPPEYLDFLGPET
RPAFDQALAVLRAQGAVIVDAPIATTDALFASPSVITVLTYEFKRDLNAYLAKVKPGTTIDTLSEVIDFNFRKREVALKY
GQGLLVDSQQRRLQDGTPITAQGYRDALAEKRLLAKTEGIDATIAKYDLDALLFPTYYGSFVGAAAGYPSVIVPAGYATG
GLPIGITFLGKAFSEPQLIQYAYAYEQASLARRPPEATP

Sequences:

>Translated_519_residues
MAAAGGLSILLGTGRAFAATCPPAGPPLAETTVAALQKALASGALTSRRIVQGYLDRIACYDKQGPKINAVLEINPDALA
IADALDAERRAGKVRGPLHGIPILLKGNIATDDRMLTTAGSVALVDSLPQKDAFIATRLREAGTVLLGKANLTEFANFMS
YYMPSGYSSQGGQTLNPYFPALEDNGVPTVTPCGSSAGSGAATAANLTAISIGTETSGSILCPSSFNSLVGIKPTVGLVS
RTGIIPISASQDVAGPMTRTVADAAVLLGAIAGYDPADPVTASSVGQIPADYRTFLKLDGLVGVRIGLPPEYLDFLGPET
RPAFDQALAVLRAQGAVIVDAPIATTDALFASPSVITVLTYEFKRDLNAYLAKVKPGTTIDTLSEVIDFNFRKREVALKY
GQGLLVDSQQRRLQDGTPITAQGYRDALAEKRLLAKTEGIDATIAKYDLDALLFPTYYGSFVGAAAGYPSVIVPAGYATG
GLPIGITFLGKAFSEPQLIQYAYAYEQASLARRPPEATP
>Mature_518_residues
AAAGGLSILLGTGRAFAATCPPAGPPLAETTVAALQKALASGALTSRRIVQGYLDRIACYDKQGPKINAVLEINPDALAI
ADALDAERRAGKVRGPLHGIPILLKGNIATDDRMLTTAGSVALVDSLPQKDAFIATRLREAGTVLLGKANLTEFANFMSY
YMPSGYSSQGGQTLNPYFPALEDNGVPTVTPCGSSAGSGAATAANLTAISIGTETSGSILCPSSFNSLVGIKPTVGLVSR
TGIIPISASQDVAGPMTRTVADAAVLLGAIAGYDPADPVTASSVGQIPADYRTFLKLDGLVGVRIGLPPEYLDFLGPETR
PAFDQALAVLRAQGAVIVDAPIATTDALFASPSVITVLTYEFKRDLNAYLAKVKPGTTIDTLSEVIDFNFRKREVALKYG
QGLLVDSQQRRLQDGTPITAQGYRDALAEKRLLAKTEGIDATIAKYDLDALLFPTYYGSFVGAAAGYPSVIVPAGYATGG
LPIGITFLGKAFSEPQLIQYAYAYEQASLARRPPEATP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=28.5433070866142, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI195972892, Length=282, Percent_Identity=28.7234042553192, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=26.6375545851528, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=532, Percent_Identity=22.9323308270677, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=352, Percent_Identity=24.1477272727273, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=443, Percent_Identity=24.3792325056433, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=237, Percent_Identity=29.957805907173, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648113, Length=521, Percent_Identity=28.9827255278311, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648435, Length=271, Percent_Identity=28.4132841328413, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648437, Length=271, Percent_Identity=28.4132841328413, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550774, Length=271, Percent_Identity=28.4132841328413, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161078093, Length=535, Percent_Identity=24.2990654205607, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644968, Length=526, Percent_Identity=24.3346007604563, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652985, Length=271, Percent_Identity=27.3062730627306, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922090, Length=271, Percent_Identity=27.3062730627306, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652981, Length=271, Percent_Identity=27.3062730627306, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652983, Length=271, Percent_Identity=27.3062730627306, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54081; Mature: 53950

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAGGLSILLGTGRAFAATCPPAGPPLAETTVAALQKALASGALTSRRIVQGYLDRIAC
CCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YDKQGPKINAVLEINPDALAIADALDAERRAGKVRGPLHGIPILLKGNIATDDRMLTTAG
CCCCCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEECCC
SVALVDSLPQKDAFIATRLREAGTVLLGKANLTEFANFMSYYMPSGYSSQGGQTLNPYFP
CCHHHHCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ALEDNGVPTVTPCGSSAGSGAATAANLTAISIGTETSGSILCPSSFNSLVGIKPTVGLVS
CCCCCCCCEECCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHHH
RTGIIPISASQDVAGPMTRTVADAAVLLGAIAGYDPADPVTASSVGQIPADYRTFLKLDG
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHEECC
LVGVRIGLPPEYLDFLGPETRPAFDQALAVLRAQGAVIVDAPIATTDALFASPSVITVLT
EEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCEEEEEE
YEFKRDLNAYLAKVKPGTTIDTLSEVIDFNFRKREVALKYGQGLLVDSQQRRLQDGTPIT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEECCHHHHHHCCCCEE
AQGYRDALAEKRLLAKTEGIDATIAKYDLDALLFPTYYGSFVGAAAGYPSVIVPAGYATG
CCCHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEECCCCCC
GLPIGITFLGKAFSEPQLIQYAYAYEQASLARRPPEATP
CCEEEHEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AAAGGLSILLGTGRAFAATCPPAGPPLAETTVAALQKALASGALTSRRIVQGYLDRIAC
CCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YDKQGPKINAVLEINPDALAIADALDAERRAGKVRGPLHGIPILLKGNIATDDRMLTTAG
CCCCCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEECCC
SVALVDSLPQKDAFIATRLREAGTVLLGKANLTEFANFMSYYMPSGYSSQGGQTLNPYFP
CCHHHHCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ALEDNGVPTVTPCGSSAGSGAATAANLTAISIGTETSGSILCPSSFNSLVGIKPTVGLVS
CCCCCCCCEECCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHHH
RTGIIPISASQDVAGPMTRTVADAAVLLGAIAGYDPADPVTASSVGQIPADYRTFLKLDG
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHEECC
LVGVRIGLPPEYLDFLGPETRPAFDQALAVLRAQGAVIVDAPIATTDALFASPSVITVLT
EEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHCCCCEEEEEE
YEFKRDLNAYLAKVKPGTTIDTLSEVIDFNFRKREVALKYGQGLLVDSQQRRLQDGTPIT
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEECCHHHHHHCCCCEE
AQGYRDALAEKRLLAKTEGIDATIAKYDLDALLFPTYYGSFVGAAAGYPSVIVPAGYATG
CCCHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEECCCCCC
GLPIGITFLGKAFSEPQLIQYAYAYEQASLARRPPEATP
CCEEEHEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]