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The map label for this gene is dmlR [H]

Identifier: 37523327

GI number: 37523327

Start: 3967485

End: 3968399

Strand: Reverse

Name: dmlR [H]

Synonym: gll3758

Alternate gene names: 37523327

Gene position: 3968399-3967485 (Counterclockwise)

Preceding gene: 37523329

Following gene: 37523323

Centisome position: 85.18

GC content: 63.28

Gene sequence:

>915_bases
ATGGAAACCGAAGCGTTGCGTCTGTTTGTCGAGGTGACCCGTCGCGGCAGCTTTGCCGCCGTCGCCAGAGACCGCCGTAT
CGATCCGTCGCTGGTGTCGCGGGCGGTGGCTTCGCTGGAGCACGATCTGGGTGTGCGTCTGTTGCAACGCACCACCCGGC
GGCTCGCCCTGACCGAAGCCGGGGAGCGCTACCTGGGCCGCATCGAGCCTCTGGTGGACGAACTGGACCGCGCGCGCGAA
GCAGTCCTGGTCCTCAGTACCGGTCCTATCGGCACATTGCGCCTCACTGCCTCGGTGGCTTTTGGTCAAAGATGCCTGGT
GCCGCTGCTGCCGCAATTGCGCTCGTCATTCCCGCAACTCAGGCTCGAACTGCTGCTCACGGATGCGAAGCTCGATCTGG
TAGGCGAGCGCATCGACCTGGCGATCCGCCTGGGACCTGCGGTCCCGGGCGACGGGGTCCGCGTCAAATGGTTCGACACG
CACTACCGAGTGTGTGCGAGTCCGGCTTATCTGCAGCGGGCACACTCCCTGCGCGATCCCGCCGAATTACCTGCCCACAG
GTGTCTACTTTTTGCCCTGCCGGAATTTCGCTCGCAGTGGCGCTTCCGGGATGCCGAGGGAATCGTGAGCGAAGTGCCCG
TCGACGGCGATATCGTCATCTCCAACGCGCTCGCGCTGCGTGAATGTACCCTGGCGGGCATGGGACCGGCGCTGTTGGCC
CACTGGCTCATCGACGAAGATATCGCCCAGGGGCGGCTGGTGGACTTGTTTCCGGCGTATCGGGCGAGTGCGACGGATTT
TGAGACTGCCGCCTGGCTGATTTATCCGAGCTGGACCTTTCTGCCCTACAAGGTGCGGGTTGTAGTCGACTTTTTGAAAC
AGTACCTCGCGGAACTGCAGCCAAGGCTTGAATGA

Upstream 100 bases:

>100_bases
ACATGTGCTTGCTCCGTTGCTTCTCGAAGCCATCCTAATCCTGTGCATGCAGACAAATAAACTAGGATCCAAGCATTGTA
TTCGTGCAGCTAATGCAGCA

Downstream 100 bases:

>100_bases
TGTCGAATCCGCAGAGACAGGCGCGCAAAGGCTCGGCAAGAGAACTGCCGACCCTCCGGCTTACCGGGTTGCTCCCGGCC
TTGACCGCCACCGCCCCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
METEALRLFVEVTRRGSFAAVARDRRIDPSLVSRAVASLEHDLGVRLLQRTTRRLALTEAGERYLGRIEPLVDELDRARE
AVLVLSTGPIGTLRLTASVAFGQRCLVPLLPQLRSSFPQLRLELLLTDAKLDLVGERIDLAIRLGPAVPGDGVRVKWFDT
HYRVCASPAYLQRAHSLRDPAELPAHRCLLFALPEFRSQWRFRDAEGIVSEVPVDGDIVISNALALRECTLAGMGPALLA
HWLIDEDIAQGRLVDLFPAYRASATDFETAAWLIYPSWTFLPYKVRVVVDFLKQYLAELQPRLE

Sequences:

>Translated_304_residues
METEALRLFVEVTRRGSFAAVARDRRIDPSLVSRAVASLEHDLGVRLLQRTTRRLALTEAGERYLGRIEPLVDELDRARE
AVLVLSTGPIGTLRLTASVAFGQRCLVPLLPQLRSSFPQLRLELLLTDAKLDLVGERIDLAIRLGPAVPGDGVRVKWFDT
HYRVCASPAYLQRAHSLRDPAELPAHRCLLFALPEFRSQWRFRDAEGIVSEVPVDGDIVISNALALRECTLAGMGPALLA
HWLIDEDIAQGRLVDLFPAYRASATDFETAAWLIYPSWTFLPYKVRVVVDFLKQYLAELQPRLE
>Mature_304_residues
METEALRLFVEVTRRGSFAAVARDRRIDPSLVSRAVASLEHDLGVRLLQRTTRRLALTEAGERYLGRIEPLVDELDRARE
AVLVLSTGPIGTLRLTASVAFGQRCLVPLLPQLRSSFPQLRLELLLTDAKLDLVGERIDLAIRLGPAVPGDGVRVKWFDT
HYRVCASPAYLQRAHSLRDPAELPAHRCLLFALPEFRSQWRFRDAEGIVSEVPVDGDIVISNALALRECTLAGMGPALLA
HWLIDEDIAQGRLVDLFPAYRASATDFETAAWLIYPSWTFLPYKVRVVVDFLKQYLAELQPRLE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=307, Percent_Identity=34.8534201954397, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=31.864406779661, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=29.3918918918919, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=27.7027027027027, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=28.2392026578073, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=25.8503401360544, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789173, Length=266, Percent_Identity=27.8195488721804, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786448, Length=273, Percent_Identity=26.007326007326, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34145; Mature: 34145

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEALRLFVEVTRRGSFAAVARDRRIDPSLVSRAVASLEHDLGVRLLQRTTRRLALTEA
CCHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GERYLGRIEPLVDELDRAREAVLVLSTGPIGTLRLTASVAFGQRCLVPLLPQLRSSFPQL
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHHHH
RLELLLTDAKLDLVGERIDLAIRLGPAVPGDGVRVKWFDTHYRVCASPAYLQRAHSLRDP
HHHHHEECCHHHHHCCEEEEEEEECCCCCCCCEEEEEEECCHHHHCCHHHHHHHHCCCCH
AELPAHRCLLFALPEFRSQWRFRDAEGIVSEVPVDGDIVISNALALRECTLAGMGPALLA
HHCCHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCCCCEEEECHHHHHHHHHCCCCHHHHH
HWLIDEDIAQGRLVDLFPAYRASATDFETAAWLIYPSWTFLPYKVRVVVDFLKQYLAELQ
HHHHHHHHHCCCEEEECHHHCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHHHHHHHC
PRLE
CCCC
>Mature Secondary Structure
METEALRLFVEVTRRGSFAAVARDRRIDPSLVSRAVASLEHDLGVRLLQRTTRRLALTEA
CCHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GERYLGRIEPLVDELDRAREAVLVLSTGPIGTLRLTASVAFGQRCLVPLLPQLRSSFPQL
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEEHHCCCHHHHHHHHHHHHHHHHH
RLELLLTDAKLDLVGERIDLAIRLGPAVPGDGVRVKWFDTHYRVCASPAYLQRAHSLRDP
HHHHHEECCHHHHHCCEEEEEEEECCCCCCCCEEEEEEECCHHHHCCHHHHHHHHCCCCH
AELPAHRCLLFALPEFRSQWRFRDAEGIVSEVPVDGDIVISNALALRECTLAGMGPALLA
HHCCHHHHHHHCCHHHHHHCCCCCCCCHHHHCCCCCCEEEECHHHHHHHHHCCCCHHHHH
HWLIDEDIAQGRLVDLFPAYRASATDFETAAWLIYPSWTFLPYKVRVVVDFLKQYLAELQ
HHHHHHHHHCCCEEEECHHHCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHHHHHHHC
PRLE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]