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The map label for this gene is btuB [H]

Identifier: 37523170

GI number: 37523170

Start: 3795077

End: 3797302

Strand: Reverse

Name: btuB [H]

Synonym: gll3601

Alternate gene names: 37523170

Gene position: 3797302-3795077 (Counterclockwise)

Preceding gene: 37523171

Following gene: 37523167

Centisome position: 81.5

GC content: 63.21

Gene sequence:

>2226_bases
ATGCATGGTTTATTCCGCCCGCACTTTGCGGGACGGTGGAACGCGGCTGCGTTCGCCTGGTGCCTTGTTTGTGTCCCAGT
TGCCCCGGCTGCTGAAGTACCGCACCTGGCCGAGCTTGAGCGGCCGGCCACCGAAGCGGGGTTGCTTACCCAGCAGCCGC
TCCCGGAGCCTCCCTCCAAAGCTCCGGCACCTGGGAGTACTCCTGCAGAACCGGTCTCGAACTTTGTCCTGGAAGATGTG
AACGTGACGGCCAACCGCCGCACCCAGAGCGATCTGAGTACGCCGCTTGCCATCACCGTGATCCCCAAAGAAGAAATCGA
CCGGCGGCGGGCGCGGGCCACGACCATCGCGGACTTGCTCAATACCGTCCCCAACTTCAGCCAGTCGTTTACGGGGGGCC
GCTTCATCAGCCAGCCGCGCCTGCGGGGCGGTCTGGGGGGGCGCGGCTCCCAGTCGGGGCTGCTCATCTTGGTGGACGGC
ATCCGCCGCAACAATACCAACGGCACCGAAGACTATCTGCTTGATTTTCCGGTCGATCAAATCGAGCGCATCGAGGTGAT
CCGGGGGGCGGCCTCGGTGCTGTACGGTACGGAAGCGGTGGACGGCGTCATCAACCTCATCACCCGCCGGGGTGTTACCG
AGCAGACCGCGCGCCTCAGCGCCAACTTCGACTTCGGCAGTTCTGAAACATTTCGCACCGGGATCGGCCTGAGCGGCAAA
GCGGGCAATCTGCGCTACCTGACGCGCTTTTCGAGCCAGAACGTCGGGGATTACCTGGATGCCACCGGGAAGACCCAGGT
GCGCAACCGCTTCGATTCTCAGAACTATCTGGCCCGCCTCACCTATGAGTTCGACAACAAAAGCGAGCTGGAATTCGAGT
TTCTCGCCTCGCGCACGCAAAATCTGCTGTTGCCCATCGTTTCTGATAGCGGAGCGGTGTTCGATTTTTCCTTTCCTTAC
CGCAACCGCGACCAGTACGCCTTCACCTACCGCAACGCAAATCTGCTCGGAAGCGAATTTCAGGCGCGGCTCTACTACAC
CGGCTTCGAGCGCAACTTCCGCAACAACATCTTCCTTGAGGCCGGACCCGGGGCATTGATCCCCACTTCGCTGCAAGACC
AGCGGCCGATTCTCACCAGCCTGGGCGGCATCGCCCAGTTCGTGAGCACCCTGGGAGCCGCCAAGCTCACCTGGGGGCTC
GACGCCTACAACGAGAGCTACTCCAACAACGACACCGAATTGACCGGCGGCGTCGCCGACTCGACTCCCCAACGCCCGAG
CGGCTCCCAGCGCTCCATCGCCCCCTTCGCCCAGGCGGCCGTGCCGATTGCTGCGGGGCTCAACCTCACCGCCGGTCTGC
GCTGGGACAACTACCGCCAAGCGGCGCAAGCCACCGCCCTCAACCCGGTCTGTCCGCTGCCTTTTTTGCCTCCTGGCGTG
CCTTGCCCCTTCCCGTTGCCGCCGGATAGCTTTCCGGGCAACGTGCGCGAGACGAGTGAACTCATCCCAGCGGCGGGCCT
CACCTGGGAATTCGCTCCAGGCTATGCGCTACGCGCCAGTTACGGCCGGGCCATCCGCAACCCGAACTTTTTTGAGTTGT
ACGGCGGACCCCAAAGCGTCGGTGTCACCCTCGGCAATCCGGGCCTCACCACCGAGCGCTCCGAGACTTACGATATCGGC
ATCAGGGTGCGCCAGGGCCGATTGCGCGCCGATATCGGTTATTTTCTGAGCAACTTCGATAACAAAATCGTCGCCCAGGC
GCTGCCCGAGTTCGGCGGGCTGGTGTTGCTGCCGGCCAACATTCGGCGGGTGCAGGGCTACGGCTACGAGGCGGAGGCGT
CCTGGGAAATCGTGGACGGCCTGACTGTATTTGGCAGCTACGGCACCGTCACCGCCTACGACCAGGACACCGGTACCCGC
ATCGGCTCTGCCCAGGGCTATCAGCTCGTGCCCGCCTCAGCAAGCATCGGGCTGCGCTTTCAGGAGCGCTCCGGCTTCTA
CGCCCAGGCCATCTTGCGCAACGTACCCAGTTACTTCGCCACCGCCCCGGCCGGTCAAGGCAACGGGTTTACCGTCCTCG
ATTTTGATCTGGGCGTGCCTTTGAGCGGGGCAACCCGCCTGGGGATCGGGGTGACCAATCTTACCGACGCTCTATATCGC
GAGCCTTTCACAACCTTCAACGCCCCCGGTGCCCAGATTTTCGCCTCGCTTGCCACGGAGTTTTGA

Upstream 100 bases:

>100_bases
CGCTGTTGGAGCGGGCGATGGGGGTCGAGTTCTATCGGTTCGAGACGCCCTTCGGCCTGCAGGTCTTTCCTATTCGGATT
CTTTCGGAGTAATTGCGCCC

Downstream 100 bases:

>100_bases
ATCAAGCTGGCAGAATATCCCAGTCTTCGGCAGCAAGGGGCTCGAAGGCATTTTCATAGAAGAAAGAGGTACCCCTCAAG
GGATAAAGACTGCGATTTTC

Product: hypothetical protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MHGLFRPHFAGRWNAAAFAWCLVCVPVAPAAEVPHLAELERPATEAGLLTQQPLPEPPSKAPAPGSTPAEPVSNFVLEDV
NVTANRRTQSDLSTPLAITVIPKEEIDRRRARATTIADLLNTVPNFSQSFTGGRFISQPRLRGGLGGRGSQSGLLILVDG
IRRNNTNGTEDYLLDFPVDQIERIEVIRGAASVLYGTEAVDGVINLITRRGVTEQTARLSANFDFGSSETFRTGIGLSGK
AGNLRYLTRFSSQNVGDYLDATGKTQVRNRFDSQNYLARLTYEFDNKSELEFEFLASRTQNLLLPIVSDSGAVFDFSFPY
RNRDQYAFTYRNANLLGSEFQARLYYTGFERNFRNNIFLEAGPGALIPTSLQDQRPILTSLGGIAQFVSTLGAAKLTWGL
DAYNESYSNNDTELTGGVADSTPQRPSGSQRSIAPFAQAAVPIAAGLNLTAGLRWDNYRQAAQATALNPVCPLPFLPPGV
PCPFPLPPDSFPGNVRETSELIPAAGLTWEFAPGYALRASYGRAIRNPNFFELYGGPQSVGVTLGNPGLTTERSETYDIG
IRVRQGRLRADIGYFLSNFDNKIVAQALPEFGGLVLLPANIRRVQGYGYEAEASWEIVDGLTVFGSYGTVTAYDQDTGTR
IGSAQGYQLVPASASIGLRFQERSGFYAQAILRNVPSYFATAPAGQGNGFTVLDFDLGVPLSGATRLGIGVTNLTDALYR
EPFTTFNAPGAQIFASLATEF

Sequences:

>Translated_741_residues
MHGLFRPHFAGRWNAAAFAWCLVCVPVAPAAEVPHLAELERPATEAGLLTQQPLPEPPSKAPAPGSTPAEPVSNFVLEDV
NVTANRRTQSDLSTPLAITVIPKEEIDRRRARATTIADLLNTVPNFSQSFTGGRFISQPRLRGGLGGRGSQSGLLILVDG
IRRNNTNGTEDYLLDFPVDQIERIEVIRGAASVLYGTEAVDGVINLITRRGVTEQTARLSANFDFGSSETFRTGIGLSGK
AGNLRYLTRFSSQNVGDYLDATGKTQVRNRFDSQNYLARLTYEFDNKSELEFEFLASRTQNLLLPIVSDSGAVFDFSFPY
RNRDQYAFTYRNANLLGSEFQARLYYTGFERNFRNNIFLEAGPGALIPTSLQDQRPILTSLGGIAQFVSTLGAAKLTWGL
DAYNESYSNNDTELTGGVADSTPQRPSGSQRSIAPFAQAAVPIAAGLNLTAGLRWDNYRQAAQATALNPVCPLPFLPPGV
PCPFPLPPDSFPGNVRETSELIPAAGLTWEFAPGYALRASYGRAIRNPNFFELYGGPQSVGVTLGNPGLTTERSETYDIG
IRVRQGRLRADIGYFLSNFDNKIVAQALPEFGGLVLLPANIRRVQGYGYEAEASWEIVDGLTVFGSYGTVTAYDQDTGTR
IGSAQGYQLVPASASIGLRFQERSGFYAQAILRNVPSYFATAPAGQGNGFTVLDFDLGVPLSGATRLGIGVTNLTDALYR
EPFTTFNAPGAQIFASLATEF
>Mature_741_residues
MHGLFRPHFAGRWNAAAFAWCLVCVPVAPAAEVPHLAELERPATEAGLLTQQPLPEPPSKAPAPGSTPAEPVSNFVLEDV
NVTANRRTQSDLSTPLAITVIPKEEIDRRRARATTIADLLNTVPNFSQSFTGGRFISQPRLRGGLGGRGSQSGLLILVDG
IRRNNTNGTEDYLLDFPVDQIERIEVIRGAASVLYGTEAVDGVINLITRRGVTEQTARLSANFDFGSSETFRTGIGLSGK
AGNLRYLTRFSSQNVGDYLDATGKTQVRNRFDSQNYLARLTYEFDNKSELEFEFLASRTQNLLLPIVSDSGAVFDFSFPY
RNRDQYAFTYRNANLLGSEFQARLYYTGFERNFRNNIFLEAGPGALIPTSLQDQRPILTSLGGIAQFVSTLGAAKLTWGL
DAYNESYSNNDTELTGGVADSTPQRPSGSQRSIAPFAQAAVPIAAGLNLTAGLRWDNYRQAAQATALNPVCPLPFLPPGV
PCPFPLPPDSFPGNVRETSELIPAAGLTWEFAPGYALRASYGRAIRNPNFFELYGGPQSVGVTLGNPGLTTERSETYDIG
IRVRQGRLRADIGYFLSNFDNKIVAQALPEFGGLVLLPANIRRVQGYGYEAEASWEIVDGLTVFGSYGTVTAYDQDTGTR
IGSAQGYQLVPASASIGLRFQERSGFYAQAILRNVPSYFATAPAGQGNGFTVLDFDLGVPLSGATRLGIGVTNLTDALYR
EPFTTFNAPGAQIFASLATEF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=138, Percent_Identity=38.4057971014493, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790405, Length=132, Percent_Identity=35.6060606060606, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 80319; Mature: 80319

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.1 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.1 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGLFRPHFAGRWNAAAFAWCLVCVPVAPAAEVPHLAELERPATEAGLLTQQPLPEPPSK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCCCC
APAPGSTPAEPVSNFVLEDVNVTANRRTQSDLSTPLAITVIPKEEIDRRRARATTIADLL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHH
NTVPNFSQSFTGGRFISQPRLRGGLGGRGSQSGLLILVDGIRRNNTNGTEDYLLDFPVDQ
HHCCCCHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHH
IERIEVIRGAASVLYGTEAVDGVINLITRRGVTEQTARLSANFDFGSSETFRTGIGLSGK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCCCEEECCCCCCC
AGNLRYLTRFSSQNVGDYLDATGKTQVRNRFDSQNYLARLTYEFDNKSELEFEFLASRTQ
CCCEEEEEECCCCCCCHHHCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCC
NLLLPIVSDSGAVFDFSFPYRNRDQYAFTYRNANLLGSEFQARLYYTGFERNFRNNIFLE
CEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEE
AGPGALIPTSLQDQRPILTSLGGIAQFVSTLGAAKLTWGLDAYNESYSNNDTELTGGVAD
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCHHHCCCCCCCCCEEECCCCC
STPQRPSGSQRSIAPFAQAAVPIAAGLNLTAGLRWDNYRQAAQATALNPVCPLPFLPPGV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCC
PCPFPLPPDSFPGNVRETSELIPAAGLTWEFAPGYALRASYGRAIRNPNFFELYGGPQSV
CCCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCCEEEHHHHHHHHCCCCEEEEECCCCEE
GVTLGNPGLTTERSETYDIGIRVRQGRLRADIGYFLSNFDNKIVAQALPEFGGLVLLPAN
EEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECC
IRRVQGYGYEAEASWEIVDGLTVFGSYGTVTAYDQDTGTRIGSAQGYQLVPASASIGLRF
CEEEECCCCCCCCCEEEECCEEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEE
QERSGFYAQAILRNVPSYFATAPAGQGNGFTVLDFDLGVPLSGATRLGIGVTNLTDALYR
ECCCCHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHHH
EPFTTFNAPGAQIFASLATEF
CCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHGLFRPHFAGRWNAAAFAWCLVCVPVAPAAEVPHLAELERPATEAGLLTQQPLPEPPSK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHCCCCCCCCCCCCCC
APAPGSTPAEPVSNFVLEDVNVTANRRTQSDLSTPLAITVIPKEEIDRRRARATTIADLL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHH
NTVPNFSQSFTGGRFISQPRLRGGLGGRGSQSGLLILVDGIRRNNTNGTEDYLLDFPVDQ
HHCCCCHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHH
IERIEVIRGAASVLYGTEAVDGVINLITRRGVTEQTARLSANFDFGSSETFRTGIGLSGK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCCCEEECCCCCCC
AGNLRYLTRFSSQNVGDYLDATGKTQVRNRFDSQNYLARLTYEFDNKSELEFEFLASRTQ
CCCEEEEEECCCCCCCHHHCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCC
NLLLPIVSDSGAVFDFSFPYRNRDQYAFTYRNANLLGSEFQARLYYTGFERNFRNNIFLE
CEEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEE
AGPGALIPTSLQDQRPILTSLGGIAQFVSTLGAAKLTWGLDAYNESYSNNDTELTGGVAD
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCHHHCCCCCCCCCEEECCCCC
STPQRPSGSQRSIAPFAQAAVPIAAGLNLTAGLRWDNYRQAAQATALNPVCPLPFLPPGV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCC
PCPFPLPPDSFPGNVRETSELIPAAGLTWEFAPGYALRASYGRAIRNPNFFELYGGPQSV
CCCCCCCCCCCCCCHHHHHHHCCCCCCEEECCCCEEEHHHHHHHHCCCCEEEEECCCCEE
GVTLGNPGLTTERSETYDIGIRVRQGRLRADIGYFLSNFDNKIVAQALPEFGGLVLLPAN
EEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECC
IRRVQGYGYEAEASWEIVDGLTVFGSYGTVTAYDQDTGTRIGSAQGYQLVPASASIGLRF
CEEEECCCCCCCCCEEEECCEEEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEEE
QERSGFYAQAILRNVPSYFATAPAGQGNGFTVLDFDLGVPLSGATRLGIGVTNLTDALYR
ECCCCHHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHHH
EPFTTFNAPGAQIFASLATEF
CCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]