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The map label for this gene is nusA [H]

Identifier: 37523058

GI number: 37523058

Start: 3699537

End: 3700688

Strand: Direct

Name: nusA [H]

Synonym: glr3489

Alternate gene names: 37523058

Gene position: 3699537-3700688 (Clockwise)

Preceding gene: 37523057

Following gene: 37523066

Centisome position: 79.41

GC content: 62.93

Gene sequence:

>1152_bases
GTGTCGATGATTCATTTGCCCGGCGTCAATCAACAGATTGAGACGATCAGCCGGCAGCGGAATTTACCCAAGCACGCCGT
CAAAGAGGCGCTGATCGAGGCGCTCAAAAAAGGTTACGAGCGCTTTCGCAAGACCTACCGCGACCCGCGCGAAAATAGCG
AAGAGATGGCCTTCGACAACTTCATCGTCGAACTCGACACCGACCGCGAGGGGTTTCGGGTGCTCGCCACCAAGACCATC
GTCGAAACGGTGAGCGACACCGACCAGGAAATCGCCCTGATTGAGGTGCGCGAAGTGGCCCCGGACGCCGAACCCGGCGG
CACGGTCGTCGTCGATGTCACCCCCGAAAAAGGTGACTTCGGCCGCATGGCCGCCATCCAGACCAAGCAGGTGCTCTCCC
AAAAACTGCGCGACCAGCAGCGCAAGCTCATTCAAGAAGAGTTCCGCGAACTGGAAGGCACGGTATTGCAGGCCCGGGTG
ATGCGCTTCGAGGGTCGCTCGGTGATCTGTGCGGTCTCCAGCGGCTTCGGCCAGAACGAAGTGGAAGCGGAATTGCCCAA
GAACGAGCAGCTCCCCAACGAGCCCTACCGGATCGGCTCGGCGTTTAAAGTCTTCCTGCGCAAAGTCTGGGAAGGTTCGC
GCCGCGGCCCGCAGCTACTCGTCTCCCGCGCCGACGCCGGGCTGGTGGTGTACCTGTTCGCCAACGAAGTGCCCGAAATT
GAAGATGAGATCGTCCGCATCGTGGCGGTCGCTCGCGAGGCGAACCCACCCTCGCGCACCGTCGGCCCGCGCACCAAGAT
CGCTGTCGATACGCTTGAGCGCGATGTGGATCCGGTGGGAGCCTGCATCGGGGCGCGCGGCTCGCGCATCCAGGCGGTCG
TCAACGAACTGCGCGGCGAAAAAATCGACGTCATCCGCTGGTCCCCCGACCCGGCCACCTACATCTCCAACGCCCTCTCA
CCGGCGCGGGTGATCCAGGTGCGCCTGGTGGACCCGGACGCGCGCCAGGCCCACGTCATCGTCCCCGACGATCAGCTGTC
GCTCGCCATCGGCAAAGAAGGCCAGAACGTCCGCCTCGCCGCCCGCCTCACCGGCTGGAAGATTGACATCAAAAAATCAA
GCGAGTACAGCGCGGAAGCGGCATATTCCTAG

Upstream 100 bases:

>100_bases
CGGCCGGCGCGTCGCGCTGCCCCGCCCCCAGGTGGCGCGGGTGCAGCTGGTGCAGTCGCTCCCTTAGACCGACAACTACA
TAGCTTCAGGAGAGTCCAAC

Downstream 100 bases:

>100_bases
CCTGTACCGTTGCTGCAGGCAGCGCTTCAGCTTTACTGCAGCAACGGCAACAACCCTTCCAACACAATTTGGGTAATTGT
CGGCAGACCCGACAGATCAC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSMIHLPGVNQQIETISRQRNLPKHAVKEALIEALKKGYERFRKTYRDPRENSEEMAFDNFIVELDTDREGFRVLATKTI
VETVSDTDQEIALIEVREVAPDAEPGGTVVVDVTPEKGDFGRMAAIQTKQVLSQKLRDQQRKLIQEEFRELEGTVLQARV
MRFEGRSVICAVSSGFGQNEVEAELPKNEQLPNEPYRIGSAFKVFLRKVWEGSRRGPQLLVSRADAGLVVYLFANEVPEI
EDEIVRIVAVAREANPPSRTVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYISNALS
PARVIQVRLVDPDARQAHVIVPDDQLSLAIGKEGQNVRLAARLTGWKIDIKKSSEYSAEAAYS

Sequences:

>Translated_383_residues
MSMIHLPGVNQQIETISRQRNLPKHAVKEALIEALKKGYERFRKTYRDPRENSEEMAFDNFIVELDTDREGFRVLATKTI
VETVSDTDQEIALIEVREVAPDAEPGGTVVVDVTPEKGDFGRMAAIQTKQVLSQKLRDQQRKLIQEEFRELEGTVLQARV
MRFEGRSVICAVSSGFGQNEVEAELPKNEQLPNEPYRIGSAFKVFLRKVWEGSRRGPQLLVSRADAGLVVYLFANEVPEI
EDEIVRIVAVAREANPPSRTVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYISNALS
PARVIQVRLVDPDARQAHVIVPDDQLSLAIGKEGQNVRLAARLTGWKIDIKKSSEYSAEAAYS
>Mature_382_residues
SMIHLPGVNQQIETISRQRNLPKHAVKEALIEALKKGYERFRKTYRDPRENSEEMAFDNFIVELDTDREGFRVLATKTIV
ETVSDTDQEIALIEVREVAPDAEPGGTVVVDVTPEKGDFGRMAAIQTKQVLSQKLRDQQRKLIQEEFRELEGTVLQARVM
RFEGRSVICAVSSGFGQNEVEAELPKNEQLPNEPYRIGSAFKVFLRKVWEGSRRGPQLLVSRADAGLVVYLFANEVPEIE
DEIVRIVAVAREANPPSRTVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYISNALSP
ARVIQVRLVDPDARQAHVIVPDDQLSLAIGKEGQNVRLAARLTGWKIDIKKSSEYSAEAAYS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=366, Percent_Identity=33.0601092896175, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42778; Mature: 42647

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIHLPGVNQQIETISRQRNLPKHAVKEALIEALKKGYERFRKTYRDPRENSEEMAFDN
CCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
FIVELDTDREGFRVLATKTIVETVSDTDQEIALIEVREVAPDAEPGGTVVVDVTPEKGDF
EEEEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCEEEEEECCCCCCC
GRMAAIQTKQVLSQKLRDQQRKLIQEEFRELEGTVLQARVMRFEGRSVICAVSSGFGQNE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
VEAELPKNEQLPNEPYRIGSAFKVFLRKVWEGSRRGPQLLVSRADAGLVVYLFANEVPEI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCEEEEEECCCCCCH
EDEIVRIVAVAREANPPSRTVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGE
HHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCC
KIDVIRWSPDPATYISNALSPARVIQVRLVDPDARQAHVIVPDDQLSLAIGKEGQNVRLA
EEEEEEECCCHHHHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEE
ARLTGWKIDIKKSSEYSAEAAYS
EEEECEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SMIHLPGVNQQIETISRQRNLPKHAVKEALIEALKKGYERFRKTYRDPRENSEEMAFDN
CEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
FIVELDTDREGFRVLATKTIVETVSDTDQEIALIEVREVAPDAEPGGTVVVDVTPEKGDF
EEEEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCCEEEEEECCCCCCC
GRMAAIQTKQVLSQKLRDQQRKLIQEEFRELEGTVLQARVMRFEGRSVICAVSSGFGQNE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
VEAELPKNEQLPNEPYRIGSAFKVFLRKVWEGSRRGPQLLVSRADAGLVVYLFANEVPEI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCEEEEEECCCCCCH
EDEIVRIVAVAREANPPSRTVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGE
HHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCC
KIDVIRWSPDPATYISNALSPARVIQVRLVDPDARQAHVIVPDDQLSLAIGKEGQNVRLA
EEEEEEECCCHHHHHHHCCCCEEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEE
ARLTGWKIDIKKSSEYSAEAAYS
EEEECEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]