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The map label for this gene is topA [H]

Identifier: 37522767

GI number: 37522767

Start: 3402505

End: 3404802

Strand: Direct

Name: topA [H]

Synonym: gvip441

Alternate gene names: 37522767

Gene position: 3402505-3404802 (Clockwise)

Preceding gene: 37522758

Following gene: 37522768

Centisome position: 73.03

GC content: 63.19

Gene sequence:

>2298_bases
ATGAGCAAATCGCTAGTCATCGTCGAATCCCCCGCCAAGGCCAAAACGATCAGCAAGATCCTCGGCAAGAACTTTGTCGT
CAAGGCCTCCGCCGGGCACATCCGCGACCTTCCCCAAAAAGAGATGGGCGTGAATGTCAAAAATGACTTCGAGCCCAAGT
ACGTGATCATTCCCAAAAAAGAAGAGGTGGTCGAGGAACTCAAAGGGGCCGCCCGCAATGCCGACCGAGTCTATCTGGCC
CCCGACCCCGACCGCGAGGGAGAAGCGATCGCCTGGCATCTGGCGCAGATTCTGGATATTCCCGGCGACAGGCTGCAGCG
CATTGAGTTTCACGAGATCACCAAGCAGGCCATCCAGAACGCCGTCGCCCATCCCCGCGACATCGATATCAACCGGGTCG
ATGCCCAGCAGGCGCGGCGCATCCTCGATCGGCTGGTGGGCTACAAGCTCAGCCCGCTGCTGTGGAAAAAAGTGCAGCGC
GGCCTGTCCGCCGGGCGGGTGCAGTCGGTGGCGGTGCGCCTGCTGTGCGACCGCGAAAAAGAAATCCAGGCGTTCGTGAG
CGAAGAGTACTGGACCGTCCACGGCCGCTTCCGCCAGAGTGCTCAGGTTGAAGCCACCCCGTTCAGCGCCGATCTGGTTC
GCTGGTCGGGCAAAAAACCGGATCTAGGCAACGAGACGGCGGCGCGGGCGGTGGTCGAAGTCCTGACGGGAGCTGCCTCG
CGGGTCGATTCGGTCAAGACCCGCGAGCGGCAAAAAGAACCCCAACCGCCCTTCATCACCAGCACCCTGCAGCGCGAAGG
CGCTTCGGCATTGGGCCTCACCGTCAAGCGCACAATGGCCATCGCCCAGCAACTCTACGAAGGCATCGATTTGGGTGAAG
AGGGGCCGGTGGGGCTGATCACCTATATGCGCACCGACAGCACCCGGGTGGCCGAAGAAGCCCAGGAAGCGGCGCGCGAA
TTTATCTTGGCCGCCTACGGCAAATCGTACTACCCGAGCCGCCGCCGCCAGTACGGCGCCAAAAAAGGCGCCCAGGACGC
CCACGAGTGCGTCCGGCCCACCGACATCAACCGCCCCCCCGACGCAATCAAAAAATCGCTCACCCCGGACCAGTTCAAGC
TCTACCGCCTGATCTGGCAGCGCTTTACGGCCTCGCAGATGACCGCGGCCACCCTGGAGACGCGCACGGTCGAAATCGCC
GCCACCCCCACAGCCGGCCGCCCCGACGCCCTGTTTCGCGTCTCGGTCACCCGCACGATCTTCGACGGCTACACCCGCGT
CTACGAAGAAGCGCGCGAGGAAGCAGCCACGGGCGACGAAGAAGCGGCCGGTGCCGCGCCGGTGCTCGAAGAGGGCGAGG
CGCTCACCTTGCTCACGGTCGACCCCAAGCAGCACTTTACCCAGCCGCCCGCGCGCTTCTCCGAGGCCACTCTCGTCAAG
GCCCTCGAAGAGCAGGGGATCGGCAGACCGAGTACCTACGCCCCCACCATCGGCACGATCCAGGAGCGCGGCTACGTCAA
TAAAGATGGCCGCACGCTCATCCCCACCGACCTGGGCATGAAAGTGAACGACCAACTGGTCCAGCACTTTCCCAACATCG
TCGATACCGAATTTACCGCCAACATGGAAGCGCAGCTCGACGAAGTCGAAAAGGGTTCCCAGCGCTGGACCCAGTTGCTT
GCGGATTTTTATGGGCCGTTTGTGGAGACGCTCAAGGCCGCCGACGAGGAGATGAAGCGGGTGGTGATCGTCACCGATCA
CCTGTGCGAGAGCTGCGGTCGGCCGCTGCTCAACCGCTACGGCCGCTTCGGCAACTTCTTGGGCTGCTCGGGCTATCCGG
AGTGCGACTTTACCCACCAGCTCACCCGCGACAACAAACCGGTCCCCAAGGACCGCCCCGCCGAGGGGATTTCCTGCAAC
CAGTGCGGCCACGCGCCGATGCTCATCACCTACGGCCGCTACGGCGAGTACCTCAAGTGCCCCGCCTGCGGCAAGAGCCA
GCCCAAATCGACGGGGATCACCTGCCCCAAGTGCAGCAAAGGCCAGATCGTCGAGCGCCGCTCGAAGATGGGCAAGAATT
TTTACGGCTGCGACCAGTATCCCGATTGCGACTTCGTGCTGTGGAGTCGGCCCATCGACAAACCCGCCTGTCCCGAGTGC
GGCTCGATCTTGCTCTACAAACCCCGCAAGCGCGGCGACGACATGGTGGCTTGTTCAGCCTGCAAATTCACCGCCCCCGC
CCAGGAGATGGTGGGCGAGGAGGAAGTGGAGCGGCAGGCTGAATTGCTTACCGGTTGA

Upstream 100 bases:

>100_bases
AGCGGCTAAGCTGTGGGCAACCCCCCATCCGCCCCGGACGGCCCCGGCTTCCCGAGGCGGACGATGGACGGTCTGTCTTG
CCACCTCGTATGTTGAGCGT

Downstream 100 bases:

>100_bases
GCGGACCGGTGCGCGCGCCAGGGAAGCTGTACGGACTGTGGGCAAAACCGTTGTTGCTGGTGCTGTCGAGTGCGGCGCTT
GTCGCCTGCGCCGGGTCACC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MSKSLVIVESPAKAKTISKILGKNFVVKASAGHIRDLPQKEMGVNVKNDFEPKYVIIPKKEEVVEELKGAARNADRVYLA
PDPDREGEAIAWHLAQILDIPGDRLQRIEFHEITKQAIQNAVAHPRDIDINRVDAQQARRILDRLVGYKLSPLLWKKVQR
GLSAGRVQSVAVRLLCDREKEIQAFVSEEYWTVHGRFRQSAQVEATPFSADLVRWSGKKPDLGNETAARAVVEVLTGAAS
RVDSVKTRERQKEPQPPFITSTLQREGASALGLTVKRTMAIAQQLYEGIDLGEEGPVGLITYMRTDSTRVAEEAQEAARE
FILAAYGKSYYPSRRRQYGAKKGAQDAHECVRPTDINRPPDAIKKSLTPDQFKLYRLIWQRFTASQMTAATLETRTVEIA
ATPTAGRPDALFRVSVTRTIFDGYTRVYEEAREEAATGDEEAAGAAPVLEEGEALTLLTVDPKQHFTQPPARFSEATLVK
ALEEQGIGRPSTYAPTIGTIQERGYVNKDGRTLIPTDLGMKVNDQLVQHFPNIVDTEFTANMEAQLDEVEKGSQRWTQLL
ADFYGPFVETLKAADEEMKRVVIVTDHLCESCGRPLLNRYGRFGNFLGCSGYPECDFTHQLTRDNKPVPKDRPAEGISCN
QCGHAPMLITYGRYGEYLKCPACGKSQPKSTGITCPKCSKGQIVERRSKMGKNFYGCDQYPDCDFVLWSRPIDKPACPEC
GSILLYKPRKRGDDMVACSACKFTAPAQEMVGEEEVERQAELLTG

Sequences:

>Translated_765_residues
MSKSLVIVESPAKAKTISKILGKNFVVKASAGHIRDLPQKEMGVNVKNDFEPKYVIIPKKEEVVEELKGAARNADRVYLA
PDPDREGEAIAWHLAQILDIPGDRLQRIEFHEITKQAIQNAVAHPRDIDINRVDAQQARRILDRLVGYKLSPLLWKKVQR
GLSAGRVQSVAVRLLCDREKEIQAFVSEEYWTVHGRFRQSAQVEATPFSADLVRWSGKKPDLGNETAARAVVEVLTGAAS
RVDSVKTRERQKEPQPPFITSTLQREGASALGLTVKRTMAIAQQLYEGIDLGEEGPVGLITYMRTDSTRVAEEAQEAARE
FILAAYGKSYYPSRRRQYGAKKGAQDAHECVRPTDINRPPDAIKKSLTPDQFKLYRLIWQRFTASQMTAATLETRTVEIA
ATPTAGRPDALFRVSVTRTIFDGYTRVYEEAREEAATGDEEAAGAAPVLEEGEALTLLTVDPKQHFTQPPARFSEATLVK
ALEEQGIGRPSTYAPTIGTIQERGYVNKDGRTLIPTDLGMKVNDQLVQHFPNIVDTEFTANMEAQLDEVEKGSQRWTQLL
ADFYGPFVETLKAADEEMKRVVIVTDHLCESCGRPLLNRYGRFGNFLGCSGYPECDFTHQLTRDNKPVPKDRPAEGISCN
QCGHAPMLITYGRYGEYLKCPACGKSQPKSTGITCPKCSKGQIVERRSKMGKNFYGCDQYPDCDFVLWSRPIDKPACPEC
GSILLYKPRKRGDDMVACSACKFTAPAQEMVGEEEVERQAELLTG
>Mature_764_residues
SKSLVIVESPAKAKTISKILGKNFVVKASAGHIRDLPQKEMGVNVKNDFEPKYVIIPKKEEVVEELKGAARNADRVYLAP
DPDREGEAIAWHLAQILDIPGDRLQRIEFHEITKQAIQNAVAHPRDIDINRVDAQQARRILDRLVGYKLSPLLWKKVQRG
LSAGRVQSVAVRLLCDREKEIQAFVSEEYWTVHGRFRQSAQVEATPFSADLVRWSGKKPDLGNETAARAVVEVLTGAASR
VDSVKTRERQKEPQPPFITSTLQREGASALGLTVKRTMAIAQQLYEGIDLGEEGPVGLITYMRTDSTRVAEEAQEAAREF
ILAAYGKSYYPSRRRQYGAKKGAQDAHECVRPTDINRPPDAIKKSLTPDQFKLYRLIWQRFTASQMTAATLETRTVEIAA
TPTAGRPDALFRVSVTRTIFDGYTRVYEEAREEAATGDEEAAGAAPVLEEGEALTLLTVDPKQHFTQPPARFSEATLVKA
LEEQGIGRPSTYAPTIGTIQERGYVNKDGRTLIPTDLGMKVNDQLVQHFPNIVDTEFTANMEAQLDEVEKGSQRWTQLLA
DFYGPFVETLKAADEEMKRVVIVTDHLCESCGRPLLNRYGRFGNFLGCSGYPECDFTHQLTRDNKPVPKDRPAEGISCNQ
CGHAPMLITYGRYGEYLKCPACGKSQPKSTGITCPKCSKGQIVERRSKMGKNFYGCDQYPDCDFVLWSRPIDKPACPECG
SILLYKPRKRGDDMVACSACKFTAPAQEMVGEEEVERQAELLTG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=829, Percent_Identity=22.6779252110977, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI10835218, Length=632, Percent_Identity=23.5759493670886, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1787529, Length=790, Percent_Identity=38.9873417721519, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1788061, Length=667, Percent_Identity=24.1379310344828, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI48994931, Length=146, Percent_Identity=41.0958904109589, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=569, Percent_Identity=24.9560632688928, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=544, Percent_Identity=25.1838235294118, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=574, Percent_Identity=24.7386759581882, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 85270; Mature: 85139

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLVIVESPAKAKTISKILGKNFVVKASAGHIRDLPQKEMGVNVKNDFEPKYVIIPKK
CCCCEEEEECCHHHHHHHHHHCCCEEEEECCCCHHCCCHHHHCCCCCCCCCCCEEEECCH
EEVVEELKGAARNADRVYLAPDPDREGEAIAWHLAQILDIPGDRLQRIEFHEITKQAIQN
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AVAHPRDIDINRVDAQQARRILDRLVGYKLSPLLWKKVQRGLSAGRVQSVAVRLLCDREK
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIQAFVSEEYWTVHGRFRQSAQVEATPFSADLVRWSGKKPDLGNETAARAVVEVLTGAAS
HHHHHHHCCCEEEEHHHCCCCCEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RVDSVKTRERQKEPQPPFITSTLQREGASALGLTVKRTMAIAQQLYEGIDLGEEGPVGLI
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TYMRTDSTRVAEEAQEAAREFILAAYGKSYYPSRRRQYGAKKGAQDAHECVRPTDINRPP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCH
DAIKKSLTPDQFKLYRLIWQRFTASQMTAATLETRTVEIAATPTAGRPDALFRVSVTRTI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEEHHHHH
FDGYTRVYEEAREEAATGDEEAAGAAPVLEEGEALTLLTVDPKQHFTQPPARFSEATLVK
HHHHHHHHHHHHHHHCCCCCHHCCCCCHHCCCCEEEEEEECCHHHCCCCCHHHHHHHHHH
ALEEQGIGRPSTYAPTIGTIQERGYVNKDGRTLIPTDLGMKVNDQLVQHFPNIVDTEFTA
HHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCCHHCCCCCC
NMEAQLDEVEKGSQRWTQLLADFYGPFVETLKAADEEMKRVVIVTDHLCESCGRPLLNRY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHCHHHHHHH
GRFGNFLGCSGYPECDFTHQLTRDNKPVPKDRPAEGISCNQCGHAPMLITYGRYGEYLKC
HCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEC
PACGKSQPKSTGITCPKCSKGQIVERRSKMGKNFYGCDQYPDCDFVLWSRPIDKPACPEC
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
GSILLYKPRKRGDDMVACSACKFTAPAQEMVGEEEVERQAELLTG
CCEEEECCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSLVIVESPAKAKTISKILGKNFVVKASAGHIRDLPQKEMGVNVKNDFEPKYVIIPKK
CCCEEEEECCHHHHHHHHHHCCCEEEEECCCCHHCCCHHHHCCCCCCCCCCCEEEECCH
EEVVEELKGAARNADRVYLAPDPDREGEAIAWHLAQILDIPGDRLQRIEFHEITKQAIQN
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AVAHPRDIDINRVDAQQARRILDRLVGYKLSPLLWKKVQRGLSAGRVQSVAVRLLCDREK
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIQAFVSEEYWTVHGRFRQSAQVEATPFSADLVRWSGKKPDLGNETAARAVVEVLTGAAS
HHHHHHHCCCEEEEHHHCCCCCEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RVDSVKTRERQKEPQPPFITSTLQREGASALGLTVKRTMAIAQQLYEGIDLGEEGPVGLI
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
TYMRTDSTRVAEEAQEAAREFILAAYGKSYYPSRRRQYGAKKGAQDAHECVRPTDINRPP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCH
DAIKKSLTPDQFKLYRLIWQRFTASQMTAATLETRTVEIAATPTAGRPDALFRVSVTRTI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCEEEEEHHHHH
FDGYTRVYEEAREEAATGDEEAAGAAPVLEEGEALTLLTVDPKQHFTQPPARFSEATLVK
HHHHHHHHHHHHHHHCCCCCHHCCCCCHHCCCCEEEEEEECCHHHCCCCCHHHHHHHHHH
ALEEQGIGRPSTYAPTIGTIQERGYVNKDGRTLIPTDLGMKVNDQLVQHFPNIVDTEFTA
HHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHCCCHHCCCCCC
NMEAQLDEVEKGSQRWTQLLADFYGPFVETLKAADEEMKRVVIVTDHLCESCGRPLLNRY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHCHHHHHHH
GRFGNFLGCSGYPECDFTHQLTRDNKPVPKDRPAEGISCNQCGHAPMLITYGRYGEYLKC
HCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEC
PACGKSQPKSTGITCPKCSKGQIVERRSKMGKNFYGCDQYPDCDFVLWSRPIDKPACPEC
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
GSILLYKPRKRGDDMVACSACKFTAPAQEMVGEEEVERQAELLTG
CCEEEECCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]