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The map label for this gene is nrgA [H]

Identifier: 37522630

GI number: 37522630

Start: 3253283

End: 3254719

Strand: Direct

Name: nrgA [H]

Synonym: glr3061

Alternate gene names: 37522630

Gene position: 3253283-3254719 (Clockwise)

Preceding gene: 37522627

Following gene: 37522635

Centisome position: 69.83

GC content: 62.14

Gene sequence:

>1437_bases
ATGTTAATGAAATGTAAATGGAAATGGGGATTGGGCACGCTGGCAGCGGCTCTCGCCACTGCCGCCCCGGCGTTTGCCCA
GGACGCGCCGAAGATCGACACCGGCGACACCGCCTGGGTGCTGGTTTCGGCCGCGCTGGTGCTGCTGATGACCCCCGGCC
TGGCCTTTTTCTACGGCGGGCTGGTGCGAGGCAAGAACGCACTGAACACTTTGATGATGAGTTTTGTTGCCCTCGGGGTG
ATCGCGCTCGTTTGGACGCTGGTGGGCTACTCGATCGCTTTTGGACCCGGTAACGGTTACTTTGGCAGCTTCGCCTGGCT
CGGCCTCAACGGGGTCGGGCAGGATCCAAACACCGCCCAGGCTGCCACCGTGCCGCACCTGGCGTTCATGATTTTTCAGA
TGATGTTTGCGGTGATTACCCCGGCGCTGATTTCCGGTGCCATCGTCGATCGCATGAAGTTCAAGACCTACGTCGTGTTC
ATCATGATCTGGTCGGTCGTGGTCTACTCGCCGGTGGCCCACTGGGTCTGGTCGCTGGGTAACCCCGACCCGACCGATGC
GGCCAAGATGCTGCCTGGCTGGCTCGGGGCTATCGGGGCGCTCGACTTTGCCGGCGGTACGGTGGTGCACCTGACGGCCG
GTATCTCGGCTTTGGTGGCGGCGATCATTCTTGGTCCGCGCAAAGGCTTTCCCAACCAGCCGATGACTCCCCACAACGTG
CCTTTTGTGCTGTTGGGAGCGGGGCTGTTGTGGTTCGGCTGGTTCGGCTTCAACGCCGGTTCGGCGCTCGCCGCCAATGG
CCTCGCTTCGCTGGCTTTTGTGACGACGAATGTCGCCACGGCGGCGGCTCTATCCACCTGGCTTTTGCTTGACACGTTTA
TCGGCGGCAAACCGACGGCGGTGGGTGCAGCCACCGGTGCGGTCGTCGGTCTGGTGGCAATCACCCCGGCGGCGGGTTTT
GTCAGCCCGCTTTCGTCGATTGCGATCGGCGGTATCGCCGCGATCATCTCCTTCGCCGCCATTCAACTGAAGAAAAAGCT
CAACTACGACGATTCGCTCGACGTGTTTGCCTGCCACGGCATGGGCGGTCTGACCGGCGCCATTCTCACTGGGGTGTTTG
CCGACAAATCGCTCAATTCCCTGGGTGATAACGGCTTGCTCCTGGGCAACGGGGCGCAGCTTATTGAGCAATTGATTGGT
GTCGGTGCCGTTGCCGTCTACGCAGCGGTCTGCACGACGGTCATTTTGCTGGCGCTCAAGTTCACCCTGGGACTGCGCCC
GAGCGAGGAAGCCGAGCAGGAGGGCCTCGACACCGCCGAGCACGGCGAGGAAGCCTACACCGGCAGCATCGGCGGCCTTG
GCGCCATGGAAGAAGCGATGTCCTCCACTTCCCAGGGCCAGGGTTTTACCCGACCGGCGACCCAGCAGCAAGAATAG

Upstream 100 bases:

>100_bases
GTAGTCATTGATACAAATGAGTCGCGACAGATTCGCTTAACTCTCCTTATTCGCCAACCGGCTGAAGCTTTTCAGTGGGC
GTGATCTCGCTCTGGGGAGA

Downstream 100 bases:

>100_bases
TTTTTCTGGCAACTGATTTGCCTGCCCCCTTTGCCAGGGGGCATTTTTTTGGTTTATTAGCGAATTGGGACTTGGCACGA
CAGTCTGTGGAGCGAGAGGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLMKCKWKWGLGTLAAALATAAPAFAQDAPKIDTGDTAWVLVSAALVLLMTPGLAFFYGGLVRGKNALNTLMMSFVALGV
IALVWTLVGYSIAFGPGNGYFGSFAWLGLNGVGQDPNTAQAATVPHLAFMIFQMMFAVITPALISGAIVDRMKFKTYVVF
IMIWSVVVYSPVAHWVWSLGNPDPTDAAKMLPGWLGAIGALDFAGGTVVHLTAGISALVAAIILGPRKGFPNQPMTPHNV
PFVLLGAGLLWFGWFGFNAGSALAANGLASLAFVTTNVATAAALSTWLLLDTFIGGKPTAVGAATGAVVGLVAITPAAGF
VSPLSSIAIGGIAAIISFAAIQLKKKLNYDDSLDVFACHGMGGLTGAILTGVFADKSLNSLGDNGLLLGNGAQLIEQLIG
VGAVAVYAAVCTTVILLALKFTLGLRPSEEAEQEGLDTAEHGEEAYTGSIGGLGAMEEAMSSTSQGQGFTRPATQQQE

Sequences:

>Translated_478_residues
MLMKCKWKWGLGTLAAALATAAPAFAQDAPKIDTGDTAWVLVSAALVLLMTPGLAFFYGGLVRGKNALNTLMMSFVALGV
IALVWTLVGYSIAFGPGNGYFGSFAWLGLNGVGQDPNTAQAATVPHLAFMIFQMMFAVITPALISGAIVDRMKFKTYVVF
IMIWSVVVYSPVAHWVWSLGNPDPTDAAKMLPGWLGAIGALDFAGGTVVHLTAGISALVAAIILGPRKGFPNQPMTPHNV
PFVLLGAGLLWFGWFGFNAGSALAANGLASLAFVTTNVATAAALSTWLLLDTFIGGKPTAVGAATGAVVGLVAITPAAGF
VSPLSSIAIGGIAAIISFAAIQLKKKLNYDDSLDVFACHGMGGLTGAILTGVFADKSLNSLGDNGLLLGNGAQLIEQLIG
VGAVAVYAAVCTTVILLALKFTLGLRPSEEAEQEGLDTAEHGEEAYTGSIGGLGAMEEAMSSTSQGQGFTRPATQQQE
>Mature_478_residues
MLMKCKWKWGLGTLAAALATAAPAFAQDAPKIDTGDTAWVLVSAALVLLMTPGLAFFYGGLVRGKNALNTLMMSFVALGV
IALVWTLVGYSIAFGPGNGYFGSFAWLGLNGVGQDPNTAQAATVPHLAFMIFQMMFAVITPALISGAIVDRMKFKTYVVF
IMIWSVVVYSPVAHWVWSLGNPDPTDAAKMLPGWLGAIGALDFAGGTVVHLTAGISALVAAIILGPRKGFPNQPMTPHNV
PFVLLGAGLLWFGWFGFNAGSALAANGLASLAFVTTNVATAAALSTWLLLDTFIGGKPTAVGAATGAVVGLVAITPAAGF
VSPLSSIAIGGIAAIISFAAIQLKKKLNYDDSLDVFACHGMGGLTGAILTGVFADKSLNSLGDNGLLLGNGAQLIEQLIG
VGAVAVYAAVCTTVILLALKFTLGLRPSEEAEQEGLDTAEHGEEAYTGSIGGLGAMEEAMSSTSQGQGFTRPATQQQE

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=445, Percent_Identity=42.0224719101124, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32564042, Length=438, Percent_Identity=29.4520547945205, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=31.447963800905, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=466, Percent_Identity=28.9699570815451, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=433, Percent_Identity=26.7898383371824, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324187, Length=456, Percent_Identity=36.6228070175439, Blast_Score=256, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=35.9154929577465, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=429, Percent_Identity=34.4988344988345, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=28.6052009456265, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49191; Mature: 49191

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMKCKWKWGLGTLAAALATAAPAFAQDAPKIDTGDTAWVLVSAALVLLMTPGLAFFYGG
CCEEEECCCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LVRGKNALNTLMMSFVALGVIALVWTLVGYSIAFGPGNGYFGSFAWLGLNGVGQDPNTAQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCCCCCCCCH
AATVPHLAFMIFQMMFAVITPALISGAIVDRMKFKTYVVFIMIWSVVVYSPVAHWVWSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPDPTDAAKMLPGWLGAIGALDFAGGTVVHLTAGISALVAAIILGPRKGFPNQPMTPHNV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVLLGAGLLWFGWFGFNAGSALAANGLASLAFVTTNVATAAALSTWLLLDTFIGGKPTA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VGAATGAVVGLVAITPAAGFVSPLSSIAIGGIAAIISFAAIQLKKKLNYDDSLDVFACHG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
MGGLTGAILTGVFADKSLNSLGDNGLLLGNGAQLIEQLIGVGAVAVYAAVCTTVILLALK
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLGLRPSEEAEQEGLDTAEHGEEAYTGSIGGLGAMEEAMSSTSQGQGFTRPATQQQE
HHHCCCCCHHHHHCCCCCHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLMKCKWKWGLGTLAAALATAAPAFAQDAPKIDTGDTAWVLVSAALVLLMTPGLAFFYGG
CCEEEECCCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LVRGKNALNTLMMSFVALGVIALVWTLVGYSIAFGPGNGYFGSFAWLGLNGVGQDPNTAQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEECCCCCCCCCCCH
AATVPHLAFMIFQMMFAVITPALISGAIVDRMKFKTYVVFIMIWSVVVYSPVAHWVWSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPDPTDAAKMLPGWLGAIGALDFAGGTVVHLTAGISALVAAIILGPRKGFPNQPMTPHNV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFVLLGAGLLWFGWFGFNAGSALAANGLASLAFVTTNVATAAALSTWLLLDTFIGGKPTA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VGAATGAVVGLVAITPAAGFVSPLSSIAIGGIAAIISFAAIQLKKKLNYDDSLDVFACHG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
MGGLTGAILTGVFADKSLNSLGDNGLLLGNGAQLIEQLIGVGAVAVYAAVCTTVILLALK
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTLGLRPSEEAEQEGLDTAEHGEEAYTGSIGGLGAMEEAMSSTSQGQGFTRPATQQQE
HHHCCCCCHHHHHCCCCCHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]