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The map label for this gene is cca [H]

Identifier: 37522493

GI number: 37522493

Start: 3119034

End: 3120467

Strand: Reverse

Name: cca [H]

Synonym: gll2924

Alternate gene names: 37522493

Gene position: 3120467-3119034 (Counterclockwise)

Preceding gene: 37522495

Following gene: 37522489

Centisome position: 66.98

GC content: 69.32

Gene sequence:

>1434_bases
GTGCTTCGAATGGGCAGTGTCCAGAGAAACTTCCAAAAGACGATGCCGCAGTTGACTTCCCTGCTCGATGCCATTCCCTT
CGCCCCGGCCGACTGGCCGGGTCCGCTGGCGCTCGTCGGCGGCAGCGTGCGCGACGCACTGTTGAACCGCACCCGCGTGC
CGCTCGATTTGGATTTCGTCTGCCCCGGCCCGGTCGCCGAGCGCGCCCGCGTTCTGGCCCGGCGGTTGGGGGCCGGTTTT
GCCGTCCTCGACGCCGAGCACGAGATCGTGCGGCTGGTGTTGGATTCGGGGATCACCCTCGATTTCGCCCGCCGGCAGGG
GGCCGACCTCGTGAGCGACCTGGCCCGCCGCGACTACACCGTCAACGCCATCGCCTGGGACGTGCATGCCGGGGAACTAT
TCGATCCCTTTGACGGCCGGGGCGATCTCACCCGCCGCACGCTGCGCGCCATCTGTGAAGCCAATCTCGTCGACGACCCG
CTGCGGTTGTTGCGGGGCTATCGCCTCGCGGCCCAGCTGGATTTTGCGATCGAGCCCGCCACCCGCGCCTGGATCGGCCT
GCACGCCCACCGGCTCGCGGCGGTCTCCGCCGAGCGGGTGCGCGAGGAACTGTGCGCCCTGATCTGCGCCCCCACCGGCG
CCGACGCGCTGCTTGCCACCTACCGCGACGGGTTACTCGCCGACTGGCTGCCGGAAATTGCTCCCATGGAGGCGATTGGA
CCGAGCGGTTACCACCACCTGCCGCTCATCGAGCACACCTTCGAGGTGATCCGCCAGGTGGACGGTGCGATTGCTGATTT
TGCCGAGCAGGTGAGAGCCGACATGGACCGGCAGGTCACAGGGGGCCACAGCGTCCGGACTCTGGTGAAGCTTGGGGCGC
TGCTGCACGACATTGCCAAGCCCCCCACCAGCAAACTCGATCCGGCGAGCGGCCGGATGAGCTTCATCGGCCACGAGAGC
CTGGGGGCGAACATGACCCGGCAGATTCTCCAGCGCCTCAAATTCAGCCGCGACGAGGAGCGCTGGGTGGCGGCCCTGGT
GCAGCACCACTTGCGCCCCGGCCAGCTGGCCGCCCACTGGCCGCCCAGCAACCGGGCGGTCTATCGCCTCTGCCGCGATC
TGGGCCAGATGCTCCCTGCCCTGCTGATGCTGGCCTTGGCCGACCGGCGTTCGACCCTCGGCCCCCAGGTCGGCCAAGAC
GACCTGGCGCGGGCAGTGGAACTGACCGGGCGGTTGCTGGGGCACTACTACACCCCCGGCGACCCGCTCGCCCACCCGCG
CACGCTCATCGACGGCAACGGGCTGATGGCCGAATTGGACCTTAAGCCGGGGCCGCGGGTGGGCCAGTTGCTCGCCGCCA
TCCAGGAGGCCCAGGCCACCGGTGAAGTGACCGGCCGCGAGGAGGCGCTGGCGCTGGCGCGGACGCTGTTGTAG

Upstream 100 bases:

>100_bases
AGGGTGTCCGAGTTACTGTGAGTGCCGTGGGCCTAAGCGGCCCGGCCCAAGACGGTCTCAGGTAACCCCGATGTCCCGGC
GTGATCCAGAGTAGCGCAAA

Downstream 100 bases:

>100_bases
GCACAAAGAAGGGCTCGGCAAACCGGAACCGCCGTAGAGCGACCCGTTCAATCTGTTCTTGTAAACAATTCCAGGGGCAA
ATGGGCGTAAGTCTCGTTGT

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLRMGSVQRNFQKTMPQLTSLLDAIPFAPADWPGPLALVGGSVRDALLNRTRVPLDLDFVCPGPVAERARVLARRLGAGF
AVLDAEHEIVRLVLDSGITLDFARRQGADLVSDLARRDYTVNAIAWDVHAGELFDPFDGRGDLTRRTLRAICEANLVDDP
LRLLRGYRLAAQLDFAIEPATRAWIGLHAHRLAAVSAERVREELCALICAPTGADALLATYRDGLLADWLPEIAPMEAIG
PSGYHHLPLIEHTFEVIRQVDGAIADFAEQVRADMDRQVTGGHSVRTLVKLGALLHDIAKPPTSKLDPASGRMSFIGHES
LGANMTRQILQRLKFSRDEERWVAALVQHHLRPGQLAAHWPPSNRAVYRLCRDLGQMLPALLMLALADRRSTLGPQVGQD
DLARAVELTGRLLGHYYTPGDPLAHPRTLIDGNGLMAELDLKPGPRVGQLLAAIQEAQATGEVTGREEALALARTLL

Sequences:

>Translated_477_residues
MLRMGSVQRNFQKTMPQLTSLLDAIPFAPADWPGPLALVGGSVRDALLNRTRVPLDLDFVCPGPVAERARVLARRLGAGF
AVLDAEHEIVRLVLDSGITLDFARRQGADLVSDLARRDYTVNAIAWDVHAGELFDPFDGRGDLTRRTLRAICEANLVDDP
LRLLRGYRLAAQLDFAIEPATRAWIGLHAHRLAAVSAERVREELCALICAPTGADALLATYRDGLLADWLPEIAPMEAIG
PSGYHHLPLIEHTFEVIRQVDGAIADFAEQVRADMDRQVTGGHSVRTLVKLGALLHDIAKPPTSKLDPASGRMSFIGHES
LGANMTRQILQRLKFSRDEERWVAALVQHHLRPGQLAAHWPPSNRAVYRLCRDLGQMLPALLMLALADRRSTLGPQVGQD
DLARAVELTGRLLGHYYTPGDPLAHPRTLIDGNGLMAELDLKPGPRVGQLLAAIQEAQATGEVTGREEALALARTLL
>Mature_477_residues
MLRMGSVQRNFQKTMPQLTSLLDAIPFAPADWPGPLALVGGSVRDALLNRTRVPLDLDFVCPGPVAERARVLARRLGAGF
AVLDAEHEIVRLVLDSGITLDFARRQGADLVSDLARRDYTVNAIAWDVHAGELFDPFDGRGDLTRRTLRAICEANLVDDP
LRLLRGYRLAAQLDFAIEPATRAWIGLHAHRLAAVSAERVREELCALICAPTGADALLATYRDGLLADWLPEIAPMEAIG
PSGYHHLPLIEHTFEVIRQVDGAIADFAEQVRADMDRQVTGGHSVRTLVKLGALLHDIAKPPTSKLDPASGRMSFIGHES
LGANMTRQILQRLKFSRDEERWVAALVQHHLRPGQLAAHWPPSNRAVYRLCRDLGQMLPALLMLALADRRSTLGPQVGQD
DLARAVELTGRLLGHYYTPGDPLAHPRTLIDGNGLMAELDLKPGPRVGQLLAAIQEAQATGEVTGREEALALARTLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=331, Percent_Identity=30.5135951661631, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52194; Mature: 52194

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRMGSVQRNFQKTMPQLTSLLDAIPFAPADWPGPLALVGGSVRDALLNRTRVPLDLDFV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCEEEECC
CPGPVAERARVLARRLGAGFAVLDAEHEIVRLVLDSGITLDFARRQGADLVSDLARRDYT
CCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHCCCE
VNAIAWDVHAGELFDPFDGRGDLTRRTLRAICEANLVDDPLRLLRGYRLAAQLDFAIEPA
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCH
TRAWIGLHAHRLAAVSAERVREELCALICAPTGADALLATYRDGLLADWLPEIAPMEAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHCC
PSGYHHLPLIEHTFEVIRQVDGAIADFAEQVRADMDRQVTGGHSVRTLVKLGALLHDIAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PPTSKLDPASGRMSFIGHESLGANMTRQILQRLKFSRDEERWVAALVQHHLRPGQLAAHW
CCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECC
PPSNRAVYRLCRDLGQMLPALLMLALADRRSTLGPQVGQDDLARAVELTGRLLGHYYTPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
DPLAHPRTLIDGNGLMAELDLKPGPRVGQLLAAIQEAQATGEVTGREEALALARTLL
CCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MLRMGSVQRNFQKTMPQLTSLLDAIPFAPADWPGPLALVGGSVRDALLNRTRVPLDLDFV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCEEEECC
CPGPVAERARVLARRLGAGFAVLDAEHEIVRLVLDSGITLDFARRQGADLVSDLARRDYT
CCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHCCCE
VNAIAWDVHAGELFDPFDGRGDLTRRTLRAICEANLVDDPLRLLRGYRLAAQLDFAIEPA
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCH
TRAWIGLHAHRLAAVSAERVREELCALICAPTGADALLATYRDGLLADWLPEIAPMEAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHCC
PSGYHHLPLIEHTFEVIRQVDGAIADFAEQVRADMDRQVTGGHSVRTLVKLGALLHDIAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PPTSKLDPASGRMSFIGHESLGANMTRQILQRLKFSRDEERWVAALVQHHLRPGQLAAHW
CCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEECC
PPSNRAVYRLCRDLGQMLPALLMLALADRRSTLGPQVGQDDLARAVELTGRLLGHYYTPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
DPLAHPRTLIDGNGLMAELDLKPGPRVGQLLAAIQEAQATGEVTGREEALALARTLL
CCCCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA