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The map label for this gene is ndhF [H]

Identifier: 37522168

GI number: 37522168

Start: 2753899

End: 2755716

Strand: Direct

Name: ndhF [H]

Synonym: gvip360

Alternate gene names: 37522168

Gene position: 2753899-2755716 (Clockwise)

Preceding gene: 37522166

Following gene: 37522169

Centisome position: 59.11

GC content: 62.6

Gene sequence:

>1818_bases
ATGGAAAGCCTTGTGCAGTTCGGTTGGCTGATCCCACTTTATTGTTTGGCGGGCGCCCTGTTTTCCCTACCCTGGGCGCT
CACCCAGTCGAAAGGCCAGCGAATTGCAGCTTTCGGCGGACTGTTCTTTACCGGGTTGGCGCTGCTGCACGCGAGCTTGA
TGTTGCCCGAGGTCCTGGCTGGAGCGGGCGGCACCTTTCGCTTTCCATGGATCGATTTTGGAACGCTGAATATCGAGGTG
AGCGGCCTGATCAACCCGCTCACCGCCGGCACACTGGTGCTGGTGGCGGGGGTGAGCCTGCTGGTGCAACTGTACTCGCT
GGGCTACATGGACCACGAGCCGCGGCTGGCCCGCTACTACGGCCTGCTCAGCTTTTTTACCATGGCCATGCTCGGGCTGG
TGCTCTCCGACAACCTGCTCGCCATGTACGGCTTCTGGGAGTTGATGGGCCTGTCGAGCTATCTGCTGGTCGGCTTCTGG
TGGTCAAAGCCCGCCGCCGCCGCCGCCGCCAAAAAAGCCTTTCTCACCACCCGCGTCGGCGACTTTTTGCTGCTGATTGG
CATTCTGCTGCTCTACGTCAACGCCGGAACGCTTGAAATTACGGCCCTGGGCGAATGGGCGGAAACGGCCACGATCGCCG
CTCCCCTCGCCACGCTGATCGTGCTATTGCTGTTCGCGGGTCCGGTGGGCAAGTCGGCCCAGTTCCCGCTGCACGTCTGG
TTGCCGGACGCGATGGAAGGTCCGACCCCGGCTTCGGCCCTCATCCACGCCGCCACGATGGTCGCCGCCGGGGTGTTCAT
GGTGGCGCGCCTCGAACCGGTCTTTGCCCTCTCGAGCACGGCCCTCATGGTCGTCGCCTACACCGGCGCCATCACCGCCC
TGGGTTCGGCCCTGATGGCCACGGCCCAAAACGACATCAAGCGGGTGCTCGCCTACTCGACCATCTCCCAGTTGGGCTAC
ATGTTCATGGCTCTGGGCGTCGGAAACACCGAGGCGGCGATGTTCCACTTGTTCACCCACGCTTTTTTCAAGGCGATGCT
GTTTCTCGGGGCCGGTTCGGTCATCCACGGCGCCCACACCCAGGACATGCGCGAGATGGGCGGTCTGATGGGCAGAATGC
CGGTGACGGCCTTCACCTACGGCATCGGTGTGCTGGCGCTTGCCGGTGTCTTTCCTTTCGCCGGTTTCTGGTCAAAGGAC
GCTATCCTGCACGCCCTCGAAAAGGCCGAACTGTATCCGGTCTTTGCGATGGGGCTGTTGACCGCCGGTCTGACCGCCTT
CTACATGGCCCGCCAGTACATCCTGGTCTTTTTGGGCAAGCCTTCTGAAAAATCGAGCCGCGCCCACGAGTCGACCTGGA
CGATGTCCCTGCCCTTGGTGATCCTGGCGGTTCCTTCGGCGGCGGCGGGCTGGCTGGGTAACGAGTGGTTCCTCAAAGAA
GAACTGGACACGACGCTTCTTGTCCTCTCCTCGGGGGTGGTCACCGTCGGCTTCGCCGTCGCCCTCGCCGTTTACGTCTT
CAATCTGGTGCCCAAGTCCGTGGTGCAGGCGCTCGTACCCGTGCGCGCCGCCCTCGAGAACCGTCTCTACATCGACGACT
TTTACAGCCGAACCTTCGGCAACGGCTTGGCGGGCATCGCCGTGGTTGTCGCCTGGCTCGACCGCAACGTGGTTGACGGC
ATCGTCAACCTGGTGAGTGCCGTGACGCTCTTGAGCGGCGAGAGCCTCAAGTACCTGGAGACCGGTAAGGCGCAGCTCTA
TCTGCTGGTGATTTTCGTCTCGGTGATCGCCCTGGGCGTAATGGTCGGAATGCGCTGA

Upstream 100 bases:

>100_bases
GAAAGGTGAAGAATGTGCGGAATGCCTTCAAAGAAGTTGTTTACGGGGCTTCTGGGCACATACAATTTCCCCCTTGGCGG
GAAGGACGGTTGCGCGACGT

Downstream 100 bases:

>100_bases
GCGGGACCGTCCTGCAAATTGGCCGATTAAAGTCTTTTAAAAGTGTGGCTTTGCTGCTGTTTCGCGGTGAGCAGGTAGTA
GTAGCCCTTGCCATTGAGGT

Product: NADH dehydrogenase subunit 5

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MESLVQFGWLIPLYCLAGALFSLPWALTQSKGQRIAAFGGLFFTGLALLHASLMLPEVLAGAGGTFRFPWIDFGTLNIEV
SGLINPLTAGTLVLVAGVSLLVQLYSLGYMDHEPRLARYYGLLSFFTMAMLGLVLSDNLLAMYGFWELMGLSSYLLVGFW
WSKPAAAAAAKKAFLTTRVGDFLLLIGILLLYVNAGTLEITALGEWAETATIAAPLATLIVLLLFAGPVGKSAQFPLHVW
LPDAMEGPTPASALIHAATMVAAGVFMVARLEPVFALSSTALMVVAYTGAITALGSALMATAQNDIKRVLAYSTISQLGY
MFMALGVGNTEAAMFHLFTHAFFKAMLFLGAGSVIHGAHTQDMREMGGLMGRMPVTAFTYGIGVLALAGVFPFAGFWSKD
AILHALEKAELYPVFAMGLLTAGLTAFYMARQYILVFLGKPSEKSSRAHESTWTMSLPLVILAVPSAAAGWLGNEWFLKE
ELDTTLLVLSSGVVTVGFAVALAVYVFNLVPKSVVQALVPVRAALENRLYIDDFYSRTFGNGLAGIAVVVAWLDRNVVDG
IVNLVSAVTLLSGESLKYLETGKAQLYLLVIFVSVIALGVMVGMR

Sequences:

>Translated_605_residues
MESLVQFGWLIPLYCLAGALFSLPWALTQSKGQRIAAFGGLFFTGLALLHASLMLPEVLAGAGGTFRFPWIDFGTLNIEV
SGLINPLTAGTLVLVAGVSLLVQLYSLGYMDHEPRLARYYGLLSFFTMAMLGLVLSDNLLAMYGFWELMGLSSYLLVGFW
WSKPAAAAAAKKAFLTTRVGDFLLLIGILLLYVNAGTLEITALGEWAETATIAAPLATLIVLLLFAGPVGKSAQFPLHVW
LPDAMEGPTPASALIHAATMVAAGVFMVARLEPVFALSSTALMVVAYTGAITALGSALMATAQNDIKRVLAYSTISQLGY
MFMALGVGNTEAAMFHLFTHAFFKAMLFLGAGSVIHGAHTQDMREMGGLMGRMPVTAFTYGIGVLALAGVFPFAGFWSKD
AILHALEKAELYPVFAMGLLTAGLTAFYMARQYILVFLGKPSEKSSRAHESTWTMSLPLVILAVPSAAAGWLGNEWFLKE
ELDTTLLVLSSGVVTVGFAVALAVYVFNLVPKSVVQALVPVRAALENRLYIDDFYSRTFGNGLAGIAVVVAWLDRNVVDG
IVNLVSAVTLLSGESLKYLETGKAQLYLLVIFVSVIALGVMVGMR
>Mature_605_residues
MESLVQFGWLIPLYCLAGALFSLPWALTQSKGQRIAAFGGLFFTGLALLHASLMLPEVLAGAGGTFRFPWIDFGTLNIEV
SGLINPLTAGTLVLVAGVSLLVQLYSLGYMDHEPRLARYYGLLSFFTMAMLGLVLSDNLLAMYGFWELMGLSSYLLVGFW
WSKPAAAAAAKKAFLTTRVGDFLLLIGILLLYVNAGTLEITALGEWAETATIAAPLATLIVLLLFAGPVGKSAQFPLHVW
LPDAMEGPTPASALIHAATMVAAGVFMVARLEPVFALSSTALMVVAYTGAITALGSALMATAQNDIKRVLAYSTISQLGY
MFMALGVGNTEAAMFHLFTHAFFKAMLFLGAGSVIHGAHTQDMREMGGLMGRMPVTAFTYGIGVLALAGVFPFAGFWSKD
AILHALEKAELYPVFAMGLLTAGLTAFYMARQYILVFLGKPSEKSSRAHESTWTMSLPLVILAVPSAAAGWLGNEWFLKE
ELDTTLLVLSSGVVTVGFAVALAVYVFNLVPKSVVQALVPVRAALENRLYIDDFYSRTFGNGLAGIAVVVAWLDRNVVDG
IVNLVSAVTLLSGESLKYLETGKAQLYLLVIFVSVIALGVMVGMR

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=376, Percent_Identity=39.6276595744681, Blast_Score=236, Evalue=5e-62,
Organism=Escherichia coli, GI1788614, Length=580, Percent_Identity=38.2758620689655, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788829, Length=419, Percent_Identity=32.4582338902148, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788827, Length=441, Percent_Identity=32.6530612244898, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=31.7258883248731, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI2367154, Length=417, Percent_Identity=28.2973621103118, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788613, Length=352, Percent_Identity=27.5568181818182, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI145693160, Length=363, Percent_Identity=26.9972451790634, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 64715; Mature: 64715

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLVQFGWLIPLYCLAGALFSLPWALTQSKGQRIAAFGGLFFTGLALLHASLMLPEVLA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
GAGGTFRFPWIDFGTLNIEVSGLINPLTAGTLVLVAGVSLLVQLYSLGYMDHEPRLARYY
CCCCCEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GLLSFFTMAMLGLVLSDNLLAMYGFWELMGLSSYLLVGFWWSKPAAAAAAKKAFLTTRVG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHHHHH
DFLLLIGILLLYVNAGTLEITALGEWAETATIAAPLATLIVLLLFAGPVGKSAQFPLHVW
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LPDAMEGPTPASALIHAATMVAAGVFMVARLEPVFALSSTALMVVAYTGAITALGSALMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHEEHHHHHHHHHHHHHHHH
TAQNDIKRVLAYSTISQLGYMFMALGVGNTEAAMFHLFTHAFFKAMLFLGAGSVIHGAHT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCH
QDMREMGGLMGRMPVTAFTYGIGVLALAGVFPFAGFWSKDAILHALEKAELYPVFAMGLL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAGLTAFYMARQYILVFLGKPSEKSSRAHESTWTMSLPLVILAVPSAAAGWLGNEWFLKE
HHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCEEEEECCCHHHCCCCCCCEEHH
ELDTTLLVLSSGVVTVGFAVALAVYVFNLVPKSVVQALVPVRAALENRLYIDDFYSRTFG
HHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NGLAGIAVVVAWLDRNVVDGIVNLVSAVTLLSGESLKYLETGKAQLYLLVIFVSVIALGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
MVGMR
HHCCC
>Mature Secondary Structure
MESLVQFGWLIPLYCLAGALFSLPWALTQSKGQRIAAFGGLFFTGLALLHASLMLPEVLA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
GAGGTFRFPWIDFGTLNIEVSGLINPLTAGTLVLVAGVSLLVQLYSLGYMDHEPRLARYY
CCCCCEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GLLSFFTMAMLGLVLSDNLLAMYGFWELMGLSSYLLVGFWWSKPAAAAAAKKAFLTTRVG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHHHHH
DFLLLIGILLLYVNAGTLEITALGEWAETATIAAPLATLIVLLLFAGPVGKSAQFPLHVW
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LPDAMEGPTPASALIHAATMVAAGVFMVARLEPVFALSSTALMVVAYTGAITALGSALMA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHEEHHHHHHHHHHHHHHHH
TAQNDIKRVLAYSTISQLGYMFMALGVGNTEAAMFHLFTHAFFKAMLFLGAGSVIHGAHT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCH
QDMREMGGLMGRMPVTAFTYGIGVLALAGVFPFAGFWSKDAILHALEKAELYPVFAMGLL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAGLTAFYMARQYILVFLGKPSEKSSRAHESTWTMSLPLVILAVPSAAAGWLGNEWFLKE
HHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCEEEEECCCHHHCCCCCCCEEHH
ELDTTLLVLSSGVVTVGFAVALAVYVFNLVPKSVVQALVPVRAALENRLYIDDFYSRTFG
HHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NGLAGIAVVVAWLDRNVVDGIVNLVSAVTLLSGESLKYLETGKAQLYLLVIFVSVIALGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
MVGMR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]