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The map label for this gene is cobA [H]

Identifier: 37522149

GI number: 37522149

Start: 2733858

End: 2734598

Strand: Direct

Name: cobA [H]

Synonym: gvip357

Alternate gene names: 37522149

Gene position: 2733858-2734598 (Clockwise)

Preceding gene: 37522148

Following gene: 37522153

Centisome position: 58.68

GC content: 67.34

Gene sequence:

>741_bases
TTGCCATCGCCTAGAGGAAAAGTCTACCTGGTGGGGGCCGGACCGGGCGATCCCGGACTTTTGACTGTGCGCGGCAAGAC
CCTCATCGAGCACTGCGATGCGATTGTCTACGACGCGCTGGTCAGCCCTGAAATTCTGGCGCTCATCCCGGCGCGTACCG
AGCGCCTCTACGCAGGAAAGCGCTCCGGGCGTCACTCGCTGACGCAAGACGAAATTACCGAGCGGCTCATCGCCCTGGTG
GAGCGGCACGCGAGCGTGGTCCGCCTCAAAGGCGGCGATCCGTTCATCTTTGGCCGGGGGGGCGAGGAAGTCGCCGCCCT
GCGCGCCGCCGGTGTCGCAGTAGAAGTGGTCCCCGGCATTACCGCCGGTATCGCCGCTCCGGCCTACGCGGGCATTCCGC
TCACCCACCGCGACATGAGCGCCTCGGTGGCCTTTGTCACCGGCCACGAGGCCGTCGAGACCTACCGTCCGGAGATCCGT
TGGGGAGAGTTGGCCCGGGGGGCTGAGACGCTGGTGATCTATATGGGCGTGCTGCAGATTGAATCGATCGTCGAGCGGTT
GTTGGCGGCGGGGCGCGCCCCCGAGACGCCCGTGGCGCTGATTCGCTGGGGAACGACTCCTCGTCAGGAGGTACTGACCG
GTACCCTCGCTACGATCGCCCCCCGGGTGCGCGCGGCCCACTTCGAGCCTCCGGCGGTGATCGTCGTGGGCGAGGTGGCG
AGACTGGCTCACACCGGCTGA

Upstream 100 bases:

>100_bases
TTTCCCAAATTCGACCTGCAACTGGATACAGTGTTGGGACCGGATAATCACCTGCTGCCGCTGATGCAGGATCTGGTGGA
AAAGACACTGGAGTCCGCTT

Downstream 100 bases:

>100_bases
TTGAAGATGACCTTTGAATAGATGGTCCGCAGGGGCAACTCCATGGCGATGGAATCGAGATGGAGAACTTCAGCCTCTTC
GAACAGTTGCACCTGCCACA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MPSPRGKVYLVGAGPGDPGLLTVRGKTLIEHCDAIVYDALVSPEILALIPARTERLYAGKRSGRHSLTQDEITERLIALV
ERHASVVRLKGGDPFIFGRGGEEVAALRAAGVAVEVVPGITAGIAAPAYAGIPLTHRDMSASVAFVTGHEAVETYRPEIR
WGELARGAETLVIYMGVLQIESIVERLLAAGRAPETPVALIRWGTTPRQEVLTGTLATIAPRVRAAHFEPPAVIVVGEVA
RLAHTG

Sequences:

>Translated_246_residues
MPSPRGKVYLVGAGPGDPGLLTVRGKTLIEHCDAIVYDALVSPEILALIPARTERLYAGKRSGRHSLTQDEITERLIALV
ERHASVVRLKGGDPFIFGRGGEEVAALRAAGVAVEVVPGITAGIAAPAYAGIPLTHRDMSASVAFVTGHEAVETYRPEIR
WGELARGAETLVIYMGVLQIESIVERLLAAGRAPETPVALIRWGTTPRQEVLTGTLATIAPRVRAAHFEPPAVIVVGEVA
RLAHTG
>Mature_245_residues
PSPRGKVYLVGAGPGDPGLLTVRGKTLIEHCDAIVYDALVSPEILALIPARTERLYAGKRSGRHSLTQDEITERLIALVE
RHASVVRLKGGDPFIFGRGGEEVAALRAAGVAVEVVPGITAGIAAPAYAGIPLTHRDMSASVAFVTGHEAVETYRPEIRW
GELARGAETLVIYMGVLQIESIVERLLAAGRAPETPVALIRWGTTPRQEVLTGTLATIAPRVRAAHFEPPAVIVVGEVAR
LAHTG

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=46.218487394958, Blast_Score=206, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=34.3096234309623, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26092; Mature: 25960

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPRGKVYLVGAGPGDPGLLTVRGKTLIEHCDAIVYDALVSPEILALIPARTERLYAGK
CCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
RSGRHSLTQDEITERLIALVERHASVVRLKGGDPFIFGRGGEEVAALRAAGVAVEVVPGI
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHCCEEEEECCCC
TAGIAAPAYAGIPLTHRDMSASVAFVTGHEAVETYRPEIRWGELARGAETLVIYMGVLQI
HHHHCCCCCCCCCEEECCCCCEEEEEECHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
ESIVERLLAAGRAPETPVALIRWGTTPRQEVLTGTLATIAPRVRAAHFEPPAVIVVGEVA
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
RLAHTG
HHHCCC
>Mature Secondary Structure 
PSPRGKVYLVGAGPGDPGLLTVRGKTLIEHCDAIVYDALVSPEILALIPARTERLYAGK
CCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
RSGRHSLTQDEITERLIALVERHASVVRLKGGDPFIFGRGGEEVAALRAAGVAVEVVPGI
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHCCEEEEECCCC
TAGIAAPAYAGIPLTHRDMSASVAFVTGHEAVETYRPEIRWGELARGAETLVIYMGVLQI
HHHHCCCCCCCCCEEECCCCCEEEEEECHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
ESIVERLLAAGRAPETPVALIRWGTTPRQEVLTGTLATIAPRVRAAHFEPPAVIVVGEVA
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
RLAHTG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]