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The map label for this gene is hisS

Identifier: 37522126

GI number: 37522126

Start: 2710452

End: 2711855

Strand: Reverse

Name: hisS

Synonym: gvip351

Alternate gene names: 37522126

Gene position: 2711855-2710452 (Counterclockwise)

Preceding gene: 37522130

Following gene: 37522125

Centisome position: 58.21

GC content: 64.74

Gene sequence:

>1404_bases
ATGGGCGAACTGGGGACGATGGGCGGCACGCGCGATTTCTTGCCCGACGAGATGATCCGCCGTGAATATGTCATCGATAC
TCTCAAAACCATCTTTCGCAAGTACGGCTTCGAACCGCTGGAGACGCCCGCGATCGAGCGCTGGGAGACCCTGGCGGGCA
AGTACGGCGAGGAGGGCGAAAAGCTCATCTACCACGTGGTGAGCAGCGGTTCGCTGGGTACCCTTAAGGCCGGCGAGCGC
ACCGAACACGCCCTGCGCTATGACTTGACGGTGCCGCTCGCGCGGGTGGTGGGCATGTACGGCGACCAGATGGTGGCCGA
CCCGGCCGATCCCAAAAAGCAAACCCGCCGCCTGCCCCGTCCCTTCAAGCGCTATCAAATCCAGCCGGTCTGGCGCGGGG
ATCGCCCCGGCGAGGGCCGCTATCGGGAGTTTCACCAGTGCGACGCCGACGTGGTCGGATCGACCAGCCCGCTGGTGGAG
ACGGAGCTGATTGCTCTGACGGTCGAAGCGTTCAAGGCCCTGGGCTTTGCCGATTTCACCGTCAAGATCAACCACCGCCA
ACTGCTGAAGGGGCTCATCGAGCAAGCTGGGATCGCACCGACTAAAGAAGCGACGGTGCTCACCTCTATCGACAAGCTCG
ACAAACTGCCGCCGGAGAAAGTGCGCCTCGAACTGGCCGGCAAGGGCCTGAATGCCGACCAGCTCGACGCCCTCTTCCAG
GTCATTGCCCTGGAAGGTACCTCTGAGCAAGTGCTGGAGGGCGCCCGTTCGCTGCTGGCTGGTTCCGAAGCCGCCCAGCG
GGGGATCGGCGAACTGACAAAGCTGCTGCATTATCTGGAGGCGCTCGGGGTGGATAGTGCCTACTACCGCATCGACCTTG
CCCTGGCGCGCGGTCTTGATTACTACACCGGCACCATCTTCGAGACGGTGAGCGCCGCGAAGGTGGGTTCGGTCGGGGCG
GGAGGCCGCTACGACCGGCTCATCTACGATCTGTCCGGCGGCAAGGCGGATCTGCCTGCCTGCGGTACCTCCTTCGGGCT
CGACCGCATCCTGGCGGCTATGGACCAGCTCGGACTTTTTGCCAGCCTCCGCCGCGCCGGCGAGGTGCTGGTGCTGCACT
TTGGCGATCCGGGCGTCTCGCAGGTGTGCTTCGAACTGGTGCGGGGACTGCGCGAGGCGGGGGTGCGCGCCGAATTGGGC
TACCACGAGGAGCCTTTCACCCCAAACGGCATGCGCCAGCAGCTGGGCTACGCCAACGAAAAAGGCTTCGCCTACGCGGT
GATCGTCGGTCCCGACGAGATGGCCCAGGGCCAGGCCGCCCTTCGGGATTTGACCACCCGCCGCCAGGAGAAAATTCCGC
TTGCGACGGCAGGACAGCTGATCGCCGGGCGTCTGCGCTCCTGA

Upstream 100 bases:

>100_bases
TGACCTTTATTATATCTGCCCTGCCCCGGGCCGGATTCTAAAACTGGCGCCGCCGCCGGGCGGGCTACTATGCTGGTTCA
GGCGGTTTGGGATTTTGGCG

Downstream 100 bases:

>100_bases
GTGGTCGCCTGTACGCTGGTGCGCGGTGGTGTCAGAATATGTGGGCACCTCCATGTGTCGCACTGTGAAGATTCGTCGCC
GTCCCGAACAGCCGCCGGTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MGELGTMGGTRDFLPDEMIRREYVIDTLKTIFRKYGFEPLETPAIERWETLAGKYGEEGEKLIYHVVSSGSLGTLKAGER
TEHALRYDLTVPLARVVGMYGDQMVADPADPKKQTRRLPRPFKRYQIQPVWRGDRPGEGRYREFHQCDADVVGSTSPLVE
TELIALTVEAFKALGFADFTVKINHRQLLKGLIEQAGIAPTKEATVLTSIDKLDKLPPEKVRLELAGKGLNADQLDALFQ
VIALEGTSEQVLEGARSLLAGSEAAQRGIGELTKLLHYLEALGVDSAYYRIDLALARGLDYYTGTIFETVSAAKVGSVGA
GGRYDRLIYDLSGGKADLPACGTSFGLDRILAAMDQLGLFASLRRAGEVLVLHFGDPGVSQVCFELVRGLREAGVRAELG
YHEEPFTPNGMRQQLGYANEKGFAYAVIVGPDEMAQGQAALRDLTTRRQEKIPLATAGQLIAGRLRS

Sequences:

>Translated_467_residues
MGELGTMGGTRDFLPDEMIRREYVIDTLKTIFRKYGFEPLETPAIERWETLAGKYGEEGEKLIYHVVSSGSLGTLKAGER
TEHALRYDLTVPLARVVGMYGDQMVADPADPKKQTRRLPRPFKRYQIQPVWRGDRPGEGRYREFHQCDADVVGSTSPLVE
TELIALTVEAFKALGFADFTVKINHRQLLKGLIEQAGIAPTKEATVLTSIDKLDKLPPEKVRLELAGKGLNADQLDALFQ
VIALEGTSEQVLEGARSLLAGSEAAQRGIGELTKLLHYLEALGVDSAYYRIDLALARGLDYYTGTIFETVSAAKVGSVGA
GGRYDRLIYDLSGGKADLPACGTSFGLDRILAAMDQLGLFASLRRAGEVLVLHFGDPGVSQVCFELVRGLREAGVRAELG
YHEEPFTPNGMRQQLGYANEKGFAYAVIVGPDEMAQGQAALRDLTTRRQEKIPLATAGQLIAGRLRS
>Mature_466_residues
GELGTMGGTRDFLPDEMIRREYVIDTLKTIFRKYGFEPLETPAIERWETLAGKYGEEGEKLIYHVVSSGSLGTLKAGERT
EHALRYDLTVPLARVVGMYGDQMVADPADPKKQTRRLPRPFKRYQIQPVWRGDRPGEGRYREFHQCDADVVGSTSPLVET
ELIALTVEAFKALGFADFTVKINHRQLLKGLIEQAGIAPTKEATVLTSIDKLDKLPPEKVRLELAGKGLNADQLDALFQV
IALEGTSEQVLEGARSLLAGSEAAQRGIGELTKLLHYLEALGVDSAYYRIDLALARGLDYYTGTIFETVSAAKVGSVGAG
GRYDRLIYDLSGGKADLPACGTSFGLDRILAAMDQLGLFASLRRAGEVLVLHFGDPGVSQVCFELVRGLREAGVRAELGY
HEEPFTPNGMRQQLGYANEKGFAYAVIVGPDEMAQGQAALRDLTTRRQEKIPLATAGQLIAGRLRS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=467, Percent_Identity=36.6167023554604, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI15029520, Length=471, Percent_Identity=33.1210191082803, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI65287717, Length=401, Percent_Identity=24.4389027431421, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788861, Length=492, Percent_Identity=27.2357723577236, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71993693, Length=480, Percent_Identity=34.375, Blast_Score=231, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI71993686, Length=480, Percent_Identity=34.375, Blast_Score=231, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6325290, Length=481, Percent_Identity=34.0956340956341, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24643061, Length=483, Percent_Identity=34.5755693581781, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24643059, Length=502, Percent_Identity=33.4661354581673, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_GLOVI (Q7NHH9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925503.1
- ProteinModelPortal:   Q7NHH9
- SMR:   Q7NHH9
- GeneID:   2600958
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2557
- HOGENOM:   HBG616575
- OMA:   LERISHT
- ProtClustDB:   PRK00037
- BioCyc:   GVIO251221:GLL2557-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 51138; Mature: 51007

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELGTMGGTRDFLPDEMIRREYVIDTLKTIFRKYGFEPLETPAIERWETLAGKYGEEGE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHH
KLIYHVVSSGSLGTLKAGERTEHALRYDLTVPLARVVGMYGDQMVADPADPKKQTRRLPR
HHHHHHHCCCCCCCCCCCCHHHHHHEEECHHHHHHHHHHCCCCEECCCCCHHHHHHCCCC
PFKRYQIQPVWRGDRPGEGRYREFHQCDADVVGSTSPLVETELIALTVEAFKALGFADFT
HHHHCEECEEECCCCCCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
VKINHRQLLKGLIEQAGIAPTKEATVLTSIDKLDKLPPEKVRLELAGKGLNADQLDALFQ
EEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHH
VIALEGTSEQVLEGARSLLAGSEAAQRGIGELTKLLHYLEALGVDSAYYRIDLALARGLD
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHCCC
YYTGTIFETVSAAKVGSVGAGGRYDRLIYDLSGGKADLPACGTSFGLDRILAAMDQLGLF
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ASLRRAGEVLVLHFGDPGVSQVCFELVRGLREAGVRAELGYHEEPFTPNGMRQQLGYANE
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCC
KGFAYAVIVGPDEMAQGQAALRDLTTRRQEKIPLATAGQLIAGRLRS
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GELGTMGGTRDFLPDEMIRREYVIDTLKTIFRKYGFEPLETPAIERWETLAGKYGEEGE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHH
KLIYHVVSSGSLGTLKAGERTEHALRYDLTVPLARVVGMYGDQMVADPADPKKQTRRLPR
HHHHHHHCCCCCCCCCCCCHHHHHHEEECHHHHHHHHHHCCCCEECCCCCHHHHHHCCCC
PFKRYQIQPVWRGDRPGEGRYREFHQCDADVVGSTSPLVETELIALTVEAFKALGFADFT
HHHHCEECEEECCCCCCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
VKINHRQLLKGLIEQAGIAPTKEATVLTSIDKLDKLPPEKVRLELAGKGLNADQLDALFQ
EEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHH
VIALEGTSEQVLEGARSLLAGSEAAQRGIGELTKLLHYLEALGVDSAYYRIDLALARGLD
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHHHCCC
YYTGTIFETVSAAKVGSVGAGGRYDRLIYDLSGGKADLPACGTSFGLDRILAAMDQLGLF
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ASLRRAGEVLVLHFGDPGVSQVCFELVRGLREAGVRAELGYHEEPFTPNGMRQQLGYANE
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCC
KGFAYAVIVGPDEMAQGQAALRDLTTRRQEKIPLATAGQLIAGRLRS
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292