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The map label for this gene is ltaA [H]

Identifier: 37522047

GI number: 37522047

Start: 2635868

End: 2636902

Strand: Direct

Name: ltaA [H]

Synonym: glr2478

Alternate gene names: 37522047

Gene position: 2635868-2636902 (Clockwise)

Preceding gene: 37522045

Following gene: 37522049

Centisome position: 56.58

GC content: 65.8

Gene sequence:

>1035_bases
ATGCCCGACCCGGTTGTCGATCTGCGCAGCGACACGGTGACCCGACCGACCCGGGCGATGCGCGCCGCCATGTTCGAAGC
CGAAGTCGGGGACGATGTCTACGGCGAAGATCCGACCGTCAACCGCCTCCAGGAGCGTGTCGCCCACCTGCTCGGCAAAG
AAAAGGGGCTATTTTTTCCGAGCGGCACGATGTGCAACCAGGTGGCCATCGCCACCCATACCCGCCCCGGCCAAGAAATC
ATCTGTGATCGCGACTGCCATGTGTTCAATTACGAGCGGGCCGGGGCAAGCCACCTGGGCGGCGTGCAACTCCATCCCCT
CGACGGCCGCCACGGGGTCCTGAGTGCCCAGCAGGTGCGCTCCGCCGTCCGCCCCATCGAGTTGCACGAACCGCCCACGG
GCTTGATTCTGCTCGAGAATACCCACAACCGCGGCGGCGGCACGGTCTATCCCCAGGACGTCATCGAACAAATCGCCGCC
GTCGCCGGCGCCGCGGGCATCCCCATGCACCTCGACGGTGCCCGGCTATTGAACGCCAGCGTCGCCGGTTCCCACAGTCC
GGCGACGCTGGCGGCACCCTTTGATTCGGTCTACTTTTGTCTTTCCAAGGGCCTCGGGTGTCCGGCGGGCTCGGTGCTCG
CCGGCAAAGCCGATTTCATCGCCCAGGCGACACGCCTGCGTAAGGCCTTTGGCGGAGGCATGCGCCAGGTGGGTTTTCTG
GCGGCTGCCGGTCTGTACGCCCTCGATCACCACATCGAGCGCCTCGCGGACGATCATCGCCGGGCACGGCAGTTGGCCGT
CCACCTAAAAGATCTCAACGCCTTCGATGTCGAGCTCGACGCTGTGCACACCAACATCGTGATGGTGGATGTGCACGCGG
ATTGCACGGCGGCGGCGGTGGCGGCGGATCTGACCGCCCACGGCGTGCGGGTGAGTGTCTTTGGTCCCCGGCGTCTGCGG
TTGGTCACCCACCTTGATATCGATGACGCGGGAGTGGAGCGGACCGTCGCCGTCTTCAAGCGGCTCTACGGCTAG

Upstream 100 bases:

>100_bases
ACCTACTTGCCATATTAGCCCCCTTTGCCAGGGGGCAGCATCTTTCTTCTGGTTTAGGTCCCGAGTCGCTAGGATGTATA
AGTACCAAGGAGTACAAGGT

Downstream 100 bases:

>100_bases
AGGGCGCTCAAAATTTCGGCCACCCGCACCTCCACCCGCGCTCCCAGGCTTTCCACGTCGGCATCGGGCTCGAAGACCCG
CGCGGGTACACCCGAGGCGG

Product: L-allo-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPDPVVDLRSDTVTRPTRAMRAAMFEAEVGDDVYGEDPTVNRLQERVAHLLGKEKGLFFPSGTMCNQVAIATHTRPGQEI
ICDRDCHVFNYERAGASHLGGVQLHPLDGRHGVLSAQQVRSAVRPIELHEPPTGLILLENTHNRGGGTVYPQDVIEQIAA
VAGAAGIPMHLDGARLLNASVAGSHSPATLAAPFDSVYFCLSKGLGCPAGSVLAGKADFIAQATRLRKAFGGGMRQVGFL
AAAGLYALDHHIERLADDHRRARQLAVHLKDLNAFDVELDAVHTNIVMVDVHADCTAAAVAADLTAHGVRVSVFGPRRLR
LVTHLDIDDAGVERTVAVFKRLYG

Sequences:

>Translated_344_residues
MPDPVVDLRSDTVTRPTRAMRAAMFEAEVGDDVYGEDPTVNRLQERVAHLLGKEKGLFFPSGTMCNQVAIATHTRPGQEI
ICDRDCHVFNYERAGASHLGGVQLHPLDGRHGVLSAQQVRSAVRPIELHEPPTGLILLENTHNRGGGTVYPQDVIEQIAA
VAGAAGIPMHLDGARLLNASVAGSHSPATLAAPFDSVYFCLSKGLGCPAGSVLAGKADFIAQATRLRKAFGGGMRQVGFL
AAAGLYALDHHIERLADDHRRARQLAVHLKDLNAFDVELDAVHTNIVMVDVHADCTAAAVAADLTAHGVRVSVFGPRRLR
LVTHLDIDDAGVERTVAVFKRLYG
>Mature_343_residues
PDPVVDLRSDTVTRPTRAMRAAMFEAEVGDDVYGEDPTVNRLQERVAHLLGKEKGLFFPSGTMCNQVAIATHTRPGQEII
CDRDCHVFNYERAGASHLGGVQLHPLDGRHGVLSAQQVRSAVRPIELHEPPTGLILLENTHNRGGGTVYPQDVIEQIAAV
AGAAGIPMHLDGARLLNASVAGSHSPATLAAPFDSVYFCLSKGLGCPAGSVLAGKADFIAQATRLRKAFGGGMRQVGFLA
AAGLYALDHHIERLADDHRRARQLAVHLKDLNAFDVELDAVHTNIVMVDVHADCTAAAVAADLTAHGVRVSVFGPRRLRL
VTHLDIDDAGVERTVAVFKRLYG

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=324, Percent_Identity=43.2098765432099, Blast_Score=244, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI193206522, Length=345, Percent_Identity=40.5797101449275, Blast_Score=237, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI193206520, Length=345, Percent_Identity=40.5797101449275, Blast_Score=237, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI193206524, Length=345, Percent_Identity=40.5797101449275, Blast_Score=237, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6320789, Length=293, Percent_Identity=39.2491467576792, Blast_Score=182, Evalue=5e-47,
Organism=Drosophila melanogaster, GI21355439, Length=298, Percent_Identity=49.3288590604027, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36879; Mature: 36748

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDPVVDLRSDTVTRPTRAMRAAMFEAEVGDDVYGEDPTVNRLQERVAHLLGKEKGLFFP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
SGTMCNQVAIATHTRPGQEIICDRDCHVFNYERAGASHLGGVQLHPLDGRHGVLSAQQVR
CCCCCCEEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHH
SAVRPIELHEPPTGLILLENTHNRGGGTVYPQDVIEQIAAVAGAAGIPMHLDGARLLNAS
HHCCCEEECCCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHCCCCCCEEECCHHHEEHH
VAGSHSPATLAAPFDSVYFCLSKGLGCPAGSVLAGKADFIAQATRLRKAFGGGMRQVGFL
CCCCCCCCEEECCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHH
AAAGLYALDHHIERLADDHRRARQLAVHLKDLNAFDVELDAVHTNIVMVDVHADCTAAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCCCHHHHH
AADLTAHGVRVSVFGPRRLRLVTHLDIDDAGVERTVAVFKRLYG
HHHHHHCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PDPVVDLRSDTVTRPTRAMRAAMFEAEVGDDVYGEDPTVNRLQERVAHLLGKEKGLFFP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
SGTMCNQVAIATHTRPGQEIICDRDCHVFNYERAGASHLGGVQLHPLDGRHGVLSAQQVR
CCCCCCEEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHH
SAVRPIELHEPPTGLILLENTHNRGGGTVYPQDVIEQIAAVAGAAGIPMHLDGARLLNAS
HHCCCEEECCCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHCCCCCCEEECCHHHEEHH
VAGSHSPATLAAPFDSVYFCLSKGLGCPAGSVLAGKADFIAQATRLRKAFGGGMRQVGFL
CCCCCCCCEEECCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHH
AAAGLYALDHHIERLADDHRRARQLAVHLKDLNAFDVELDAVHTNIVMVDVHADCTAAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCCCHHHHH
AADLTAHGVRVSVFGPRRLRLVTHLDIDDAGVERTVAVFKRLYG
HHHHHHCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]