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The map label for this gene is dfrA [H]

Identifier: 37522045

GI number: 37522045

Start: 2634903

End: 2635412

Strand: Direct

Name: dfrA [H]

Synonym: glr2476

Alternate gene names: 37522045

Gene position: 2634903-2635412 (Clockwise)

Preceding gene: 37522041

Following gene: 37522047

Centisome position: 56.55

GC content: 58.24

Gene sequence:

>510_bases
ATGGAATTGATCGCCGTCGTAGCCATGGACAGTCGCCGCCTAATCGGACGGGGCGATGGTCTACCCTGGCACTGCCCGGA
AGATCTTAGTGCGTTCAAAGCACTGACCTGGGGCCAACCTTTGCTGATGGGCCGCCGAACCTTCGAGAGCATCCTCGCCC
GCCGAGGCAAACCTTTGCCCGAGCGCGAACATTATGTGCTGACGGCCAGACGCGGACCGTCCTTTCCAACGGTCCGCTAT
ATTTGCTCACCGGATCAGGCGCTTGCTCTGCCTGTCGAACGCCTGTTCGTGATTGGCGGCGCTTCGGTGTACGCCCAAAC
CGCCGACCAGCTAGATACCCTTTACCTGAGTTTGATCCCCGGCTTGCACACGGGCGATGTCTTCCTGCCGGACTTGGGAA
ACCGGTGGGCAGTGGAAATTATTGAAAAATTTCAAACCTTTAAACGTTGGCGCCTACTGCGCAGTCCTTCTGTGGGCTTC
GATTGGTCCCAGCTTGTCGAAGGCGGCTAG

Upstream 100 bases:

>100_bases
GCTTTCGGCTTGGCGTTGTTGCAACCCCGCTGCTCTATCGCTGGAGATTCGGCATTGGGCGGCAAGGGGGAAGCCTATCG
GACCAGTGTTGAACTTGCCC

Downstream 100 bases:

>100_bases
CGGTCGCGGCGTTCGTCGTCGGGAGAACGGTTGTACAAAGCTTCGAGGCGGCTGCGGATGTCGGTGTCGCGGGGGGTGAC
ACGCACCACCAGCAGCGAGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MELIAVVAMDSRRLIGRGDGLPWHCPEDLSAFKALTWGQPLLMGRRTFESILARRGKPLPEREHYVLTARRGPSFPTVRY
ICSPDQALALPVERLFVIGGASVYAQTADQLDTLYLSLIPGLHTGDVFLPDLGNRWAVEIIEKFQTFKRWRLLRSPSVGF
DWSQLVEGG

Sequences:

>Translated_169_residues
MELIAVVAMDSRRLIGRGDGLPWHCPEDLSAFKALTWGQPLLMGRRTFESILARRGKPLPEREHYVLTARRGPSFPTVRY
ICSPDQALALPVERLFVIGGASVYAQTADQLDTLYLSLIPGLHTGDVFLPDLGNRWAVEIIEKFQTFKRWRLLRSPSVGF
DWSQLVEGG
>Mature_169_residues
MELIAVVAMDSRRLIGRGDGLPWHCPEDLSAFKALTWGQPLLMGRRTFESILARRGKPLPEREHYVLTARRGPSFPTVRY
ICSPDQALALPVERLFVIGGASVYAQTADQLDTLYLSLIPGLHTGDVFLPDLGNRWAVEIIEKFQTFKRWRLLRSPSVGF
DWSQLVEGG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=133, Percent_Identity=39.0977443609023, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19035; Mature: 19035

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIAVVAMDSRRLIGRGDGLPWHCPEDLSAFKALTWGQPLLMGRRTFESILARRGKPLP
CCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
EREHYVLTARRGPSFPTVRYICSPDQALALPVERLFVIGGASVYAQTADQLDTLYLSLIP
CCCCEEEEECCCCCCCEEEEEECCCCHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHCC
GLHTGDVFLPDLGNRWAVEIIEKFQTFKRWRLLRSPSVGFDWSQLVEGG
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MELIAVVAMDSRRLIGRGDGLPWHCPEDLSAFKALTWGQPLLMGRRTFESILARRGKPLP
CCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
EREHYVLTARRGPSFPTVRYICSPDQALALPVERLFVIGGASVYAQTADQLDTLYLSLIP
CCCCEEEEECCCCCCCEEEEEECCCCHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHCC
GLHTGDVFLPDLGNRWAVEIIEKFQTFKRWRLLRSPSVGFDWSQLVEGG
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2548057; 2365064 [H]