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The map label for this gene is ndhJ [H]

Identifier: 37521949

GI number: 37521949

Start: 2538835

End: 2539374

Strand: Direct

Name: ndhJ [H]

Synonym: gvip332

Alternate gene names: 37521949

Gene position: 2538835-2539374 (Clockwise)

Preceding gene: 37521948

Following gene: 37521950

Centisome position: 54.49

GC content: 62.78

Gene sequence:

>540_bases
ATGGAGAACACCGAGACGCCCGCGCCTGAGGGGGCGCAAACCGGCACCGAGATTACCCCGATCGAGCCGGGGCCGACCTC
CCGCTGGCTCGCCGAGCACGACTTGCCCCACGCATTTTTGGGCTTTGCCCTGGACGGGGTCGAACTGGTGGGCGTCGAAC
CCGAGCACCTGCCCATGGTCGGCAAAGCCCTGCACGATCAGGGCTACAACTATCTCAGGCTGATGTGCGGCTACGACGAG
GGGCCGGGCGAGCGGCTGGTGAGCGTCTATGGCCTTACCCACTGCTACGACGACGCTGACAAACCGCCGGAGGTGCGCCT
CAAGGTCTTTCTCGACCGTGCTGACCCGCGCGTGTCCTCGGTCTACTGGATCTGGAAGGCGGCGGACTGGCAGGAGCGCG
AGACCTACGACATGTACGGCATCGTCTACGAAGGCCACCCCAACCTCATCCGCATCCTGAACATGGAAGATATGGTGGGC
TGGCCATTGCGCAAAGATTATGTGACGCCGGGATTTTACGAGGTTCAGGACGCATTTTAG

Upstream 100 bases:

>100_bases
GAAAGCCGCCAGGCCGCCCCGCCCGCCCTGGCCGCCGCCATCGAGATGGGCATTCCCCTGGACTTGCAGCGCACGCCCCA
ACTGGAGGAGCAGATCCGCG

Downstream 100 bases:

>100_bases
GGCAAACTTGATCCAGCGGTCAGCAAAAGACACCGAGGCGCAGTCGCCACGCAAAGCGTGCACGCATAGGAAATATCAGC
CAAGCCTACCCACCCAGTTT

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MENTETPAPEGAQTGTEITPIEPGPTSRWLAEHDLPHAFLGFALDGVELVGVEPEHLPMVGKALHDQGYNYLRLMCGYDE
GPGERLVSVYGLTHCYDDADKPPEVRLKVFLDRADPRVSSVYWIWKAADWQERETYDMYGIVYEGHPNLIRILNMEDMVG
WPLRKDYVTPGFYEVQDAF

Sequences:

>Translated_179_residues
MENTETPAPEGAQTGTEITPIEPGPTSRWLAEHDLPHAFLGFALDGVELVGVEPEHLPMVGKALHDQGYNYLRLMCGYDE
GPGERLVSVYGLTHCYDDADKPPEVRLKVFLDRADPRVSSVYWIWKAADWQERETYDMYGIVYEGHPNLIRILNMEDMVG
WPLRKDYVTPGFYEVQDAF
>Mature_179_residues
MENTETPAPEGAQTGTEITPIEPGPTSRWLAEHDLPHAFLGFALDGVELVGVEPEHLPMVGKALHDQGYNYLRLMCGYDE
GPGERLVSVYGLTHCYDDADKPPEVRLKVFLDRADPRVSSVYWIWKAADWQERETYDMYGIVYEGHPNLIRILNMEDMVG
WPLRKDYVTPGFYEVQDAF

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI145693162, Length=130, Percent_Identity=33.8461538461538, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32563621, Length=116, Percent_Identity=36.2068965517241, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71990788, Length=116, Percent_Identity=36.2068965517241, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010218
- InterPro:   IPR001268
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa [H]

EC number: 1.6.5.3

Molecular weight: Translated: 20405; Mature: 20405

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTETPAPEGAQTGTEITPIEPGPTSRWLAEHDLPHAFLGFALDGVELVGVEPEHLPMV
CCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCHHHHHEEECCEEEECCCCHHCCHH
GKALHDQGYNYLRLMCGYDEGPGERLVSVYGLTHCYDDADKPPEVRLKVFLDRADPRVSS
HHHHHHCCCCEEEEEECCCCCCHHHEEHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEE
VYWIWKAADWQERETYDMYGIVYEGHPNLIRILNMEDMVGWPLRKDYVTPGFYEVQDAF
EEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MENTETPAPEGAQTGTEITPIEPGPTSRWLAEHDLPHAFLGFALDGVELVGVEPEHLPMV
CCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCHHHHHEEECCEEEECCCCHHCCHH
GKALHDQGYNYLRLMCGYDEGPGERLVSVYGLTHCYDDADKPPEVRLKVFLDRADPRVSS
HHHHHHCCCCEEEEEECCCCCCHHHEEHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEE
VYWIWKAADWQERETYDMYGIVYEGHPNLIRILNMEDMVGWPLRKDYVTPGFYEVQDAF
EEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]