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The map label for this gene is ndhK

Identifier: 37521948

GI number: 37521948

Start: 2538114

End: 2538863

Strand: Direct

Name: ndhK

Synonym: gvip331

Alternate gene names: 37521948

Gene position: 2538114-2538863 (Clockwise)

Preceding gene: 37521946

Following gene: 37521949

Centisome position: 54.48

GC content: 64.0

Gene sequence:

>750_bases
ATGTTCAACGACCGACCGCAGACCGACCGGATGTTCTTTCCGCCCGAACGCCCGGAAGTAACCAGCGAACTGAGCAACAA
CGTGGTGCTCACGACGCTCAACGATCTGTACAACTGGGCGCGGCTTTCGAGCGTCTGGCCTCTGATGTACGGCACGGCCT
GCTGCTTTATCGAATTTGCCGGACTGATCGGCTCGCGCTTCGACTTCGACCGCTTCGGGCTGGTGCCCCGCGCCTCACCG
CGCCAGGCGGACCTGATCATCACCGCAGGCACGATCACGATGAAATTTGCCCCGGCCCTGGTGACGCTCTACCAGCAGAT
GCCCGAGCCCAAGTACGTGATCGCCATGGGCGCGTGCACGATCACCGGCGGCATGTTCTCCACCGACTCGCCCACCACCG
TGCGCGGCGTCGATAAGCTCATACCGGTGGACGTGTACATTCCGGGTTGCCCGCCCAGGCCCGAGGCGATCTTCGATGCG
ATCATCAAATTGCGCAAGAAGATGGCCACCGAGGATTTTCGCGAGCGCTACGACACAATTGCCCAGACCCACCGCTACTA
CACCGCCGCGCACCGGATGAAGGCCGTGGCCGACCCGCTCACCAGTGAGTACATTCGCCTGGAAAGCCGCCAGGCCGCCC
CGCCCGCCCTGGCCGCCGCCATCGAGATGGGCATTCCCCTGGACTTGCAGCGCACGCCCCAACTGGAGGAGCAGATCCGC
GATGGAGAACACCGAGACGCCCGCGCCTGA

Upstream 100 bases:

>100_bases
GGGCCCGGGGAGCGGGTAGGCACCTCTGCCGAAAGCGGGAGGATACTGGAGGGGGCGGTTCTTAGACTAAGCTCAGGCCA
TCGTCCGCCCGGGGGACCCC

Downstream 100 bases:

>100_bases
GGGGGCGCAAACCGGCACCGAGATTACCCCGATCGAGCCGGGGCCGACCTCCCGCTGGCTCGCCGAGCACGACTTGCCCC
ACGCATTTTTGGGCTTTGCC

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K 2; NDH-1 subunit K 2; NDH-K 2

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MFNDRPQTDRMFFPPERPEVTSELSNNVVLTTLNDLYNWARLSSVWPLMYGTACCFIEFAGLIGSRFDFDRFGLVPRASP
RQADLIITAGTITMKFAPALVTLYQQMPEPKYVIAMGACTITGGMFSTDSPTTVRGVDKLIPVDVYIPGCPPRPEAIFDA
IIKLRKKMATEDFRERYDTIAQTHRYYTAAHRMKAVADPLTSEYIRLESRQAAPPALAAAIEMGIPLDLQRTPQLEEQIR
DGEHRDARA

Sequences:

>Translated_249_residues
MFNDRPQTDRMFFPPERPEVTSELSNNVVLTTLNDLYNWARLSSVWPLMYGTACCFIEFAGLIGSRFDFDRFGLVPRASP
RQADLIITAGTITMKFAPALVTLYQQMPEPKYVIAMGACTITGGMFSTDSPTTVRGVDKLIPVDVYIPGCPPRPEAIFDA
IIKLRKKMATEDFRERYDTIAQTHRYYTAAHRMKAVADPLTSEYIRLESRQAAPPALAAAIEMGIPLDLQRTPQLEEQIR
DGEHRDARA
>Mature_249_residues
MFNDRPQTDRMFFPPERPEVTSELSNNVVLTTLNDLYNWARLSSVWPLMYGTACCFIEFAGLIGSRFDFDRFGLVPRASP
RQADLIITAGTITMKFAPALVTLYQQMPEPKYVIAMGACTITGGMFSTDSPTTVRGVDKLIPVDVYIPGCPPRPEAIFDA
IIKLRKKMATEDFRERYDTIAQTHRYYTAAHRMKAVADPLTSEYIRLESRQAAPPALAAAIEMGIPLDLQRTPQLEEQIR
DGEHRDARA

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=172, Percent_Identity=47.093023255814, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1788624, Length=172, Percent_Identity=46.5116279069767, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=40.7407407407407, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788834, Length=129, Percent_Identity=34.8837209302326, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=139, Percent_Identity=50.3597122302158, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=53.2374100719424, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=53.2374100719424, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=52.2058823529412, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK2_GLOVI (Q7NI05)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_925325.1
- ProteinModelPortal:   Q7NI05
- SMR:   Q7NI05
- GeneID:   2600780
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.2379
- HOGENOM:   HBG553221
- OMA:   SENIVLT
- ProtClustDB:   PRK06411
- BioCyc:   GVIO251221:GLR2379-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 28040; Mature: 28040

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNDRPQTDRMFFPPERPEVTSELSNNVVLTTLNDLYNWARLSSVWPLMYGTACCFIEFA
CCCCCCCCCCEECCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIGSRFDFDRFGLVPRASPRQADLIITAGTITMKFAPALVTLYQQMPEPKYVIAMGACT
HHHCCCCCHHHHCCCCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHCCCCCEEEEECCEE
ITGGMFSTDSPTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIIKLRKKMATEDFRERYDTI
EECCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQTHRYYTAAHRMKAVADPLTSEYIRLESRQAAPPALAAAIEMGIPLDLQRTPQLEEQIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHC
DGEHRDARA
CCCCCCCCC
>Mature Secondary Structure
MFNDRPQTDRMFFPPERPEVTSELSNNVVLTTLNDLYNWARLSSVWPLMYGTACCFIEFA
CCCCCCCCCCEECCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIGSRFDFDRFGLVPRASPRQADLIITAGTITMKFAPALVTLYQQMPEPKYVIAMGACT
HHHCCCCCHHHHCCCCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHCCCCCEEEEECCEE
ITGGMFSTDSPTTVRGVDKLIPVDVYIPGCPPRPEAIFDAIIKLRKKMATEDFRERYDTI
EECCCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQTHRYYTAAHRMKAVADPLTSEYIRLESRQAAPPALAAAIEMGIPLDLQRTPQLEEQIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHC
DGEHRDARA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292